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The map label for this gene is thiG [H]

Identifier: 73539914

GI number: 73539914

Start: 235438

End: 236274

Strand: Direct

Name: thiG [H]

Synonym: Reut_A0208

Alternate gene names: 73539914

Gene position: 235438-236274 (Clockwise)

Preceding gene: 73539913

Following gene: 73539915

Centisome position: 6.19

GC content: 68.7

Gene sequence:

>837_bases
ATGACATTCGAACCGACTGAAACCCTGCGCGACCCGTTCGTGCTCTATGGCGAGTCCTTCGACTCGCGCCTGCTGCTGGG
CACGGCCCGCTATCCGTCGCCGGCCACGCTGCAGGCAGCCGTGGAAATTTCGCGCCCGGCCATGGTGACCGTGGCACTGC
GCCGCCAGGGCGCGGTGGGCGAAGGCGAGGGTGGCCAGGCCTTCTGGCAGATGCTCAAGGCGCTCGGCGTGCCCGTGCTG
CCGAACACCGCCGGCTGTTTCACGCCGCAGGAAGTGGTCAACACCGCCATGATGGCGCGCGAGGTGTTCGAGACCGACTG
GATCAAGCTCGAGCTGATCGGCGACGACTACACGCTGCAGCCCGACACGCTGAACCTGCCCGACGTGGCCGAAACGCTGA
TCAAGGAAGGCTTCAAGGTGCTGCCGTACTGCACTGAGGACCTCGTCCTGTGCCGCCGTCTGCTCGACGTCGGCTGCCAG
GCACTGATGCCGTGGGCCGCGCCGATCGGCACCGGCCGTGGCGCCATCAACCCGCACGCGATGCGCGTACTGCGCGAACG
CCTGCCGGACACCCCGCTGATCGTCGACGCAGGCCTGGGTCTGCCGTCGCATGCGGCGCAGGTGCTGGAGTGGGGCTATG
ACGGCGTGCTGCTGAACACCGCTGTGGCGCAGGCCGCGTATCCGGTCAACATGGCACGCGCGTTCGCGCAGGCCGTCGAA
GCCGGACGCACCGCCTATCTGGCCGGCCCGATGCCCGAGCGCGAAGTCGCGCAGGCGAGCACCCCGGTGGTCGGCATGCC
GTTCTGGCACGCCGAAAACACGGAGCACCGCGCATGA

Upstream 100 bases:

>100_bases
TCAACGGCCAGTTCGTGCCCCGCGCCGCGCACGCGGCCACGCCCCTCGCGGCCGGCGACCGCATTGACCTCGTCCAACCC
GTGACGGGAGGCTGACCCCC

Downstream 100 bases:

>100_bases
CCCGCCACGCGGTGCACGCCCCGAGCGACGGCCCGCTGCGCCAGGCCGTGCTTGAACACTACGGCGAGACCTTCGGCGTG
TCGGATCATCCGTGGCAGGC

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MTFEPTETLRDPFVLYGESFDSRLLLGTARYPSPATLQAAVEISRPAMVTVALRRQGAVGEGEGGQAFWQMLKALGVPVL
PNTAGCFTPQEVVNTAMMAREVFETDWIKLELIGDDYTLQPDTLNLPDVAETLIKEGFKVLPYCTEDLVLCRRLLDVGCQ
ALMPWAAPIGTGRGAINPHAMRVLRERLPDTPLIVDAGLGLPSHAAQVLEWGYDGVLLNTAVAQAAYPVNMARAFAQAVE
AGRTAYLAGPMPEREVAQASTPVVGMPFWHAENTEHRA

Sequences:

>Translated_278_residues
MTFEPTETLRDPFVLYGESFDSRLLLGTARYPSPATLQAAVEISRPAMVTVALRRQGAVGEGEGGQAFWQMLKALGVPVL
PNTAGCFTPQEVVNTAMMAREVFETDWIKLELIGDDYTLQPDTLNLPDVAETLIKEGFKVLPYCTEDLVLCRRLLDVGCQ
ALMPWAAPIGTGRGAINPHAMRVLRERLPDTPLIVDAGLGLPSHAAQVLEWGYDGVLLNTAVAQAAYPVNMARAFAQAVE
AGRTAYLAGPMPEREVAQASTPVVGMPFWHAENTEHRA
>Mature_277_residues
TFEPTETLRDPFVLYGESFDSRLLLGTARYPSPATLQAAVEISRPAMVTVALRRQGAVGEGEGGQAFWQMLKALGVPVLP
NTAGCFTPQEVVNTAMMAREVFETDWIKLELIGDDYTLQPDTLNLPDVAETLIKEGFKVLPYCTEDLVLCRRLLDVGCQA
LMPWAAPIGTGRGAINPHAMRVLRERLPDTPLIVDAGLGLPSHAAQVLEWGYDGVLLNTAVAQAAYPVNMARAFAQAVEA
GRTAYLAGPMPEREVAQASTPVVGMPFWHAENTEHRA

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=249, Percent_Identity=44.578313253012, Blast_Score=199, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 30203; Mature: 30072

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFEPTETLRDPFVLYGESFDSRLLLGTARYPSPATLQAAVEISRPAMVTVALRRQGAVG
CCCCCCHHHCCCEEEECCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEEEEHHCCCCC
EGEGGQAFWQMLKALGVPVLPNTAGCFTPQEVVNTAMMAREVFETDWIKLELIGDDYTLQ
CCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEC
PDTLNLPDVAETLIKEGFKVLPYCTEDLVLCRRLLDVGCQALMPWAAPIGTGRGAINPHA
CCCCCCHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
MRVLRERLPDTPLIVDAGLGLPSHAAQVLEWGYDGVLLNTAVAQAAYPVNMARAFAQAVE
HHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCEEHHHHHHHHHCCHHHHHHHHHHHH
AGRTAYLAGPMPEREVAQASTPVVGMPFWHAENTEHRA
HCCEEEEECCCCHHHHHHCCCCEEECCEECCCCCCCCC
>Mature Secondary Structure 
TFEPTETLRDPFVLYGESFDSRLLLGTARYPSPATLQAAVEISRPAMVTVALRRQGAVG
CCCCCHHHCCCEEEECCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEEEEHHCCCCC
EGEGGQAFWQMLKALGVPVLPNTAGCFTPQEVVNTAMMAREVFETDWIKLELIGDDYTLQ
CCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEC
PDTLNLPDVAETLIKEGFKVLPYCTEDLVLCRRLLDVGCQALMPWAAPIGTGRGAINPHA
CCCCCCHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
MRVLRERLPDTPLIVDAGLGLPSHAAQVLEWGYDGVLLNTAVAQAAYPVNMARAFAQAVE
HHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCEEHHHHHHHHHCCHHHHHHHHHHHH
AGRTAYLAGPMPEREVAQASTPVVGMPFWHAENTEHRA
HCCEEEEECCCCHHHHHHCCCCEEECCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA