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The map label for this gene is dapF

Identifier: 73539904

GI number: 73539904

Start: 222866

End: 223732

Strand: Reverse

Name: dapF

Synonym: Reut_A0198

Alternate gene names: 73539904

Gene position: 223732-222866 (Counterclockwise)

Preceding gene: 73539905

Following gene: 73539903

Centisome position: 5.88

GC content: 66.21

Gene sequence:

>867_bases
ATGAAACTCCAGTTCACCAAGATGCACGGTGCCGGCAACGACTTCGTCGTGCTCGACGGCATCCACCAGAAGCTCGACCT
GACCACCGAACAGTGGCGTGCGCTTGCCAGCCGGCATTTCGGCGTCGGCGCCGACCAGATGCTGATCGTGGAAAAGCCCA
CGCGCGAGGACGTGGATTTCCGCTACCGCATCTTCAATGCCGATGGCAGCGAGGTCGAGCACTGCGGCAATGGCGCGCGC
TGCTTCGTGCGCTTCGTCACCGACAAGGGCATGACCGACAAGCGCTCGGTGCGCGTGGAGGTCATGAACGGCGTGATCAC
GCTGACACTGCAGGACGACGGCCAGGTCACCGTGGACATGGGCGCGCCCGAACTGGAACCGGCTCGCGTGCCGTTCCGCC
CGGATGGCCTGCCAGTCCGCACGCAAGGTGAAGACATCGCCTACGGGTTGGAAATCAACGGCCGCACGGCGTGGATTTCC
CCGGTGTCGATGGGCAATCCGCACGCAGTGCAGGTGGTGGACGATGTCGAGAACTTCCCGGTACTGCAGGACGGTCCGTT
GATCGAACATCACGCGACGTTCCCGAACCGCGTGAACGCCGGCTTCCTGCAAGTGGTGGACCGCCATACCGCGCGCCTGC
GCGTGTTCGAACGCGGCGCCGGCGAGACGCTGGCCTGCGGCACGGGCGCTTGCGCGGCCGTCGTAGCCGGCATTCGCCGC
GGCCTGCTGGATTCCCCGGTCAAGGTCCACACGCACGGTGGCGACCTGAACATTGCCTGGGACGGCGGCGCCGAACCCGT
GCGCATGACCGGTCCGGCTACCACCGTGTTCGAAGGCACCATTGACCTCGCCGCCCTGCCGGCCTGA

Upstream 100 bases:

>100_bases
TACTGGGTGCACAAGCGCTTCAAGAACCGCCCGCCCGGCGAACCGTCCATCTACTGACTACCGCCGGCAGGTCCGGCCCT
GCGCGGCTACAATTCGCACC

Downstream 100 bases:

>100_bases
CTATAGGGGCCTGTGCGCCCCGCTTCGCATCCTCGTGTCCCGACTGCGGCGGGGTTCCCCGAACGTTCCGCGCGGTGCCC
TGCCGCCGGACGCACCGATG

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKLQFTKMHGAGNDFVVLDGIHQKLDLTTEQWRALASRHFGVGADQMLIVEKPTREDVDFRYRIFNADGSEVEHCGNGAR
CFVRFVTDKGMTDKRSVRVEVMNGVITLTLQDDGQVTVDMGAPELEPARVPFRPDGLPVRTQGEDIAYGLEINGRTAWIS
PVSMGNPHAVQVVDDVENFPVLQDGPLIEHHATFPNRVNAGFLQVVDRHTARLRVFERGAGETLACGTGACAAVVAGIRR
GLLDSPVKVHTHGGDLNIAWDGGAEPVRMTGPATTVFEGTIDLAALPA

Sequences:

>Translated_288_residues
MKLQFTKMHGAGNDFVVLDGIHQKLDLTTEQWRALASRHFGVGADQMLIVEKPTREDVDFRYRIFNADGSEVEHCGNGAR
CFVRFVTDKGMTDKRSVRVEVMNGVITLTLQDDGQVTVDMGAPELEPARVPFRPDGLPVRTQGEDIAYGLEINGRTAWIS
PVSMGNPHAVQVVDDVENFPVLQDGPLIEHHATFPNRVNAGFLQVVDRHTARLRVFERGAGETLACGTGACAAVVAGIRR
GLLDSPVKVHTHGGDLNIAWDGGAEPVRMTGPATTVFEGTIDLAALPA
>Mature_288_residues
MKLQFTKMHGAGNDFVVLDGIHQKLDLTTEQWRALASRHFGVGADQMLIVEKPTREDVDFRYRIFNADGSEVEHCGNGAR
CFVRFVTDKGMTDKRSVRVEVMNGVITLTLQDDGQVTVDMGAPELEPARVPFRPDGLPVRTQGEDIAYGLEINGRTAWIS
PVSMGNPHAVQVVDDVENFPVLQDGPLIEHHATFPNRVNAGFLQVVDRHTARLRVFERGAGETLACGTGACAAVVAGIRR
GLLDSPVKVHTHGGDLNIAWDGGAEPVRMTGPATTVFEGTIDLAALPA

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=281, Percent_Identity=53.0249110320285, Blast_Score=293, Evalue=9e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_CUPPJ (Q476V3)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_294424.1
- HSSP:   P44859
- ProteinModelPortal:   Q476V3
- SMR:   Q476V3
- GeneID:   3610530
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A0198
- NMPDR:   fig|264198.3.peg.710
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- BioCyc:   REUT264198:REUT_A0198-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 31259; Mature: 31259

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 75-75 ACT_SITE 226-226

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQFTKMHGAGNDFVVLDGIHQKLDLTTEQWRALASRHFGVGADQMLIVEKPTREDVDF
CCEEEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCE
RYRIFNADGSEVEHCGNGARCFVRFVTDKGMTDKRSVRVEVMNGVITLTLQDDGQVTVDM
EEEEECCCCCHHHHCCCCCEEEEEEEECCCCCCCCEEEEEEECCEEEEEECCCCCEEEEC
GAPELEPARVPFRPDGLPVRTQGEDIAYGLEINGRTAWISPVSMGNPHAVQVVDDVENFP
CCCCCCCCCCCCCCCCCEEEECCCCEEEEEEECCCEEEEEECCCCCCEEEEEEECCCCCC
VLQDGPLIEHHATFPNRVNAGFLQVVDRHTARLRVFERGAGETLACGTGACAAVVAGIRR
EECCCCEEEECCCCCCCCCCHHHHHHHHCCEEEEEEECCCCCEEEECCCHHHHHHHHHHH
GLLDSPVKVHTHGGDLNIAWDGGAEPVRMTGPATTVFEGTIDLAALPA
HHCCCCEEEEECCCEEEEEECCCCCCEEEECCCEEEEECCEEEEECCC
>Mature Secondary Structure
MKLQFTKMHGAGNDFVVLDGIHQKLDLTTEQWRALASRHFGVGADQMLIVEKPTREDVDF
CCEEEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCE
RYRIFNADGSEVEHCGNGARCFVRFVTDKGMTDKRSVRVEVMNGVITLTLQDDGQVTVDM
EEEEECCCCCHHHHCCCCCEEEEEEEECCCCCCCCEEEEEEECCEEEEEECCCCCEEEEC
GAPELEPARVPFRPDGLPVRTQGEDIAYGLEINGRTAWISPVSMGNPHAVQVVDDVENFP
CCCCCCCCCCCCCCCCCEEEECCCCEEEEEEECCCEEEEEECCCCCCEEEEEEECCCCCC
VLQDGPLIEHHATFPNRVNAGFLQVVDRHTARLRVFERGAGETLACGTGACAAVVAGIRR
EECCCCEEEECCCCCCCCCCHHHHHHHHCCEEEEEEECCCCCEEEECCCHHHHHHHHHHH
GLLDSPVKVHTHGGDLNIAWDGGAEPVRMTGPATTVFEGTIDLAALPA
HHCCCCEEEEECCCEEEEEECCCCCCEEEECCCEEEEECCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA