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The map label for this gene is htrB [H]

Identifier: 73539905

GI number: 73539905

Start: 223776

End: 224669

Strand: Reverse

Name: htrB [H]

Synonym: Reut_A0199

Alternate gene names: 73539905

Gene position: 224669-223776 (Counterclockwise)

Preceding gene: 73539906

Following gene: 73539904

Centisome position: 5.9

GC content: 65.1

Gene sequence:

>894_bases
ATGAGCCGTATCTTTACCTGGCTCGGCATCGGTCTGCTGACCGTGCTGGGCAAGCTGCCCTACCCGTTCGTCGCCCGTTT
CGGCGAGGGGCTGGGCAGCCTGCTATATCTCTTTCCGAGCAGCCGGCGCGGGATCGTGCAGGCCAACCTGCGCGCCAGCT
TTCCCGACAAGACCGAAGCCGAGATCGACACGCTGTCGCGTGATTCCTTCCGCACCGTGTTCCGCAGCTTTGCCGAGCGC
GGCATCTTCTGGACCGGAAGCGAGGCACAGATGCGCCGCTGGGTACAGATCGACGACCAGGCGGATCTGGTCGCGCTCGA
CGGTACACCCCACATCCTGGTCACGCTCCACCTGTCCGGCGTGGAAGCCGGTGCGATCCGCCTGACCATCCACCTGCGGG
ACCACGTCGGTCGTTCGGGTGCATCGCTGTACACCGCGCAGAAGAACACGCTGTTCGACGGGTTCCTCAAGCACGCACGG
GGCCGCTTTGGCGCGAAGATGATCTCGCGCAACGACAGCGCGCGCGACATCCTGCGCTGCCTGAAGAAAGGCGAAGCACT
GCAGCTCATCGCCGACATGGACTTCGGCGAGCGCGATTCGGAGTATGTGCCGTTCTTCGGCGTGCAGGCGCTGACGCTGA
CCTCGGTGTCGCGCCTGGCGCGGATGACGGGCGCCAAGGTCGTGCCGGTCTACACGGAGATGCTGCCCGACTACCAGGGC
TACGTGCTTCGCATCTTGCCGGCCTGGGACAACTACCCCGGCGACAGCGTGACGGAGGACACGCGCCGCATGAACGCGTT
CTTTGAAGACTGCATCCGCCCGCGCGTGGCCGAGTACTACTGGGTGCACAAGCGCTTCAAGAACCGCCCGCCCGGCGAAC
CGTCCATCTACTGA

Upstream 100 bases:

>100_bases
CGCCCACGCAGTACCTCTGGGGCTATCACCGCTACAAGACTCCGTCCGGCGTGCAGGACCCCGATCGGGGCACGCTGGCG
GCTACCGAATCCTCCCGCTC

Downstream 100 bases:

>100_bases
CTACCGCCGGCAGGTCCGGCCCTGCGCGGCTACAATTCGCACCATGAAACTCCAGTTCACCAAGATGCACGGTGCCGGCA
ACGACTTCGTCGTGCTCGAC

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSRIFTWLGIGLLTVLGKLPYPFVARFGEGLGSLLYLFPSSRRGIVQANLRASFPDKTEAEIDTLSRDSFRTVFRSFAER
GIFWTGSEAQMRRWVQIDDQADLVALDGTPHILVTLHLSGVEAGAIRLTIHLRDHVGRSGASLYTAQKNTLFDGFLKHAR
GRFGAKMISRNDSARDILRCLKKGEALQLIADMDFGERDSEYVPFFGVQALTLTSVSRLARMTGAKVVPVYTEMLPDYQG
YVLRILPAWDNYPGDSVTEDTRRMNAFFEDCIRPRVAEYYWVHKRFKNRPPGEPSIY

Sequences:

>Translated_297_residues
MSRIFTWLGIGLLTVLGKLPYPFVARFGEGLGSLLYLFPSSRRGIVQANLRASFPDKTEAEIDTLSRDSFRTVFRSFAER
GIFWTGSEAQMRRWVQIDDQADLVALDGTPHILVTLHLSGVEAGAIRLTIHLRDHVGRSGASLYTAQKNTLFDGFLKHAR
GRFGAKMISRNDSARDILRCLKKGEALQLIADMDFGERDSEYVPFFGVQALTLTSVSRLARMTGAKVVPVYTEMLPDYQG
YVLRILPAWDNYPGDSVTEDTRRMNAFFEDCIRPRVAEYYWVHKRFKNRPPGEPSIY
>Mature_296_residues
SRIFTWLGIGLLTVLGKLPYPFVARFGEGLGSLLYLFPSSRRGIVQANLRASFPDKTEAEIDTLSRDSFRTVFRSFAERG
IFWTGSEAQMRRWVQIDDQADLVALDGTPHILVTLHLSGVEAGAIRLTIHLRDHVGRSGASLYTAQKNTLFDGFLKHARG
RFGAKMISRNDSARDILRCLKKGEALQLIADMDFGERDSEYVPFFGVQALTLTSVSRLARMTGAKVVPVYTEMLPDYQGY
VLRILPAWDNYPGDSVTEDTRRMNAFFEDCIRPRVAEYYWVHKRFKNRPPGEPSIY

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=296, Percent_Identity=34.4594594594595, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI87082094, Length=296, Percent_Identity=29.7297297297297, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1788161, Length=308, Percent_Identity=26.9480519480519, Blast_Score=101, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 33682; Mature: 33551

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIFTWLGIGLLTVLGKLPYPFVARFGEGLGSLLYLFPSSRRGIVQANLRASFPDKTEA
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEECCCCCCCEEEECCCCCCCCCCHH
EIDTLSRDSFRTVFRSFAERGIFWTGSEAQMRRWVQIDDQADLVALDGTPHILVTLHLSG
HHHHHCHHHHHHHHHHHHHCCCEEECCHHHHHHHEEECCCCCEEEECCCCEEEEEEEECC
VEAGAIRLTIHLRDHVGRSGASLYTAQKNTLFDGFLKHARGRFGAKMISRNDSARDILRC
CCCCEEEEEEEEHHHCCCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHH
LKKGEALQLIADMDFGERDSEYVPFFGVQALTLTSVSRLARMTGAKVVPVYTEMLPDYQG
HHCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEHHHHHCCCCCC
YVLRILPAWDNYPGDSVTEDTRRMNAFFEDCIRPRVAEYYWVHKRFKNRPPGEPSIY
EEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SRIFTWLGIGLLTVLGKLPYPFVARFGEGLGSLLYLFPSSRRGIVQANLRASFPDKTEA
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEECCCCCCCEEEECCCCCCCCCCHH
EIDTLSRDSFRTVFRSFAERGIFWTGSEAQMRRWVQIDDQADLVALDGTPHILVTLHLSG
HHHHHCHHHHHHHHHHHHHCCCEEECCHHHHHHHEEECCCCCEEEECCCCEEEEEEEECC
VEAGAIRLTIHLRDHVGRSGASLYTAQKNTLFDGFLKHARGRFGAKMISRNDSARDILRC
CCCCEEEEEEEEHHHCCCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHH
LKKGEALQLIADMDFGERDSEYVPFFGVQALTLTSVSRLARMTGAKVVPVYTEMLPDYQG
HHCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEHHHHHCCCCCC
YVLRILPAWDNYPGDSVTEDTRRMNAFFEDCIRPRVAEYYWVHKRFKNRPPGEPSIY
EEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]