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The map label for this gene is pyrE [H]

Identifier: 73539901

GI number: 73539901

Start: 219845

End: 220546

Strand: Direct

Name: pyrE [H]

Synonym: Reut_A0195

Alternate gene names: 73539901

Gene position: 219845-220546 (Clockwise)

Preceding gene: 73539898

Following gene: 73539902

Centisome position: 5.78

GC content: 65.81

Gene sequence:

>702_bases
ATGACGCAGCAGACGACGACTTCGACCACGCAGCAGGCGGGCCAGCCGGATCTCAGCCAGACCTTTATCCGCTTCGCGCT
GGACGCCGGCGTGCTGTCCTTTGGCGAATTCGTCACCAAGGCGGGCCGCAAGTCGCCGTACTTCTTCAATGCCGGCCTGT
TCAACCAGGGCGCCATGCTCGGCCAGGTGGCGCAATTCTATGCGAAAACCCTGCTGGCCTCGGGCGTGCAGTTCGACGTG
CTGTTCGGGCCGGCCTACAAGGGCATCACGCTCGCGTCTGCCACCGCAGTGGCGCTGGCGGGCATGGGACGTGACGTCGG
CTTCGCCTATAACCGCAAGGAAGCCAAGGACCATGGCGAGGGCGGTACGCTGGTCGGGGCAAAGCTCCAGGGCAAGGTCG
TCATCATCGACGATGTGATCTCCGCCGGCACTTCGGTGCGCGAATCGATGCAGCTGATCCGGGCGGCCGGCGCCGAACCG
GCCGCCGTGCTGATCGCGCTGGACCGGATGGAAAAGAGCGGTACCGCCGACCAGATCGGCACCCATTCGGCCGTGCAGGA
CGTGCAGCGCGAATACGGCGTGCCCGTGATCGCCATTGCCAGCCTGAAGGATCTGCTGGCCTATCTGGACGCGTCCAACA
ATCCCTCGCTGTCGGCCTCGCGCGAGGCTGTGGCCGCCTATCGCCAGCGCTACGGCGTCTGA

Upstream 100 bases:

>100_bases
CACTCGAAAAAGCCCTTCTTGGAAGCGGAGCGGATGCCGTTGAGGTTGGCGCTGATAATCCGTAACATGCGGGAAAATTT
TTGAATTCAGAGAGAAGAGC

Downstream 100 bases:

>100_bases
CCGGCACTGTGGCGCGCGGCTGACCGGAGGTAGCTGCCGGCGTGCCGCGCCCAAGGCAGGATTCACCATCTGTGAAGGAG
CAAGGCGTGGCGGGCAAAGA

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MTQQTTTSTTQQAGQPDLSQTFIRFALDAGVLSFGEFVTKAGRKSPYFFNAGLFNQGAMLGQVAQFYAKTLLASGVQFDV
LFGPAYKGITLASATAVALAGMGRDVGFAYNRKEAKDHGEGGTLVGAKLQGKVVIIDDVISAGTSVRESMQLIRAAGAEP
AAVLIALDRMEKSGTADQIGTHSAVQDVQREYGVPVIAIASLKDLLAYLDASNNPSLSASREAVAAYRQRYGV

Sequences:

>Translated_233_residues
MTQQTTTSTTQQAGQPDLSQTFIRFALDAGVLSFGEFVTKAGRKSPYFFNAGLFNQGAMLGQVAQFYAKTLLASGVQFDV
LFGPAYKGITLASATAVALAGMGRDVGFAYNRKEAKDHGEGGTLVGAKLQGKVVIIDDVISAGTSVRESMQLIRAAGAEP
AAVLIALDRMEKSGTADQIGTHSAVQDVQREYGVPVIAIASLKDLLAYLDASNNPSLSASREAVAAYRQRYGV
>Mature_232_residues
TQQTTTSTTQQAGQPDLSQTFIRFALDAGVLSFGEFVTKAGRKSPYFFNAGLFNQGAMLGQVAQFYAKTLLASGVQFDVL
FGPAYKGITLASATAVALAGMGRDVGFAYNRKEAKDHGEGGTLVGAKLQGKVVIIDDVISAGTSVRESMQLIRAAGAEPA
AVLIALDRMEKSGTADQIGTHSAVQDVQREYGVPVIAIASLKDLLAYLDASNNPSLSASREAVAAYRQRYGV

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=185, Percent_Identity=26.4864864864865, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1790073, Length=215, Percent_Identity=55.8139534883721, Blast_Score=239, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6323530, Length=221, Percent_Identity=43.8914027149321, Blast_Score=191, Evalue=9e-50,
Organism=Saccharomyces cerevisiae, GI6323927, Length=222, Percent_Identity=41.8918918918919, Blast_Score=181, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 24535; Mature: 24404

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQTTTSTTQQAGQPDLSQTFIRFALDAGVLSFGEFVTKAGRKSPYFFNAGLFNQGAML
CCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHH
GQVAQFYAKTLLASGVQFDVLFGPAYKGITLASATAVALAGMGRDVGFAYNRKEAKDHGE
HHHHHHHHHHHHHCCCEEEEEECCCCCCEEHHHHHHHHHHHCCCCCCCCCCCHHHHCCCC
GGTLVGAKLQGKVVIIDDVISAGTSVRESMQLIRAAGAEPAAVLIALDRMEKSGTADQIG
CCEEEEEEECCCEEEEECHHHCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHCCCCHHHHC
THSAVQDVQREYGVPVIAIASLKDLLAYLDASNNPSLSASREAVAAYRQRYGV
CHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TQQTTTSTTQQAGQPDLSQTFIRFALDAGVLSFGEFVTKAGRKSPYFFNAGLFNQGAML
CCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHH
GQVAQFYAKTLLASGVQFDVLFGPAYKGITLASATAVALAGMGRDVGFAYNRKEAKDHGE
HHHHHHHHHHHHHCCCEEEEEECCCCCCEEHHHHHHHHHHHCCCCCCCCCCCHHHHCCCC
GGTLVGAKLQGKVVIIDDVISAGTSVRESMQLIRAAGAEPAAVLIALDRMEKSGTADQIG
CCEEEEEEECCCEEEEECHHHCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHCCCCHHHHC
THSAVQDVQREYGVPVIAIASLKDLLAYLDASNNPSLSASREAVAAYRQRYGV
CHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA