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The map label for this gene is exoA [H]

Identifier: 73539900

GI number: 73539900

Start: 219036

End: 219812

Strand: Reverse

Name: exoA [H]

Synonym: Reut_A0194

Alternate gene names: 73539900

Gene position: 219812-219036 (Counterclockwise)

Preceding gene: 73539903

Following gene: 73539899

Centisome position: 5.77

GC content: 59.59

Gene sequence:

>777_bases
ATGTTACGGATTATCAGCGCCAACCTCAACGGCATCCGCTCCGCTTCCAAGAAGGGCTTTTTCGAGTGGATGGGCAAGCA
GGACGCGGACATGGTCTGCGTGCAGGAACTCAAGGCCCAGGCCGCCGACATGACCGAAGCGTTCTTGGCGCCACATGGCT
ACCACGGCTACTTCCACTACGCCGAGAAGAAGGGATACAGCGGTGTCGGCCTCTACACACGCCACAAGCCGGAGCGCGTG
ATCACGGGCTTTGGCAACCCTGAGTTCGACAGTGAAGGCCGTTACGTGGAGGTGCAGTATCCGCACCTCGCGGTGATCTC
GGTGTATGTTCCGTCGGGCTCAAGCTCGGAGGAGCGCCAGCAGGCAAAGTTCCGCTTCATGGAGGCGTTCCTGCCTCACC
TGTTGCAACTGAAGGCCAGTGGGCGTGAGATCGTGCTGTGCGGCGACGTGAACATCGCGCACAAGGAAATCGATATCAAG
AACTGGAAGGGCAACCTGAAGAACTCGGGCTTCCTGCCGGAAGAACGGGCCTGGATCGGTCAGTTGTTCGACATGCACGG
CTACGTGGACGTGTTCCGCCAGCTGGATCCCCGCCCCGAGCAATACACATGGTGGAGCAACCGCGGCCAGGCCTATGCCA
AGAACGTCGGGTGGCGCATCGATTACCACCTGGCCACGCCGAAGATTGCCGGAACGGCGCATGTCTGCTCGATCTACAAG
GATGAGAAGTTCAGCGACCACGCGCCGCTGTCGATCGATTACAACTATCCTCTGTGA

Upstream 100 bases:

>100_bases
ATAAAGGTCTGGCTGAGATCCGGCTGGCCCGCCTGCTGCGTGGTCGAAGTCGTCGTCTGCTGCGTCATGCTCTTCTCTCT
GAATTCAAAAATTTTCCCGC

Downstream 100 bases:

>100_bases
CGGGTCAGGGCGGTCATGCCGCCCTGACTTCCTCTAGCAGTTCCGGGGGGCGGTCCAGCAAGCCACTAACCCTGCCAAAT
CACAAGAATCCAATCGGTAC

Product: AP endonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLRIISANLNGIRSASKKGFFEWMGKQDADMVCVQELKAQAADMTEAFLAPHGYHGYFHYAEKKGYSGVGLYTRHKPERV
ITGFGNPEFDSEGRYVEVQYPHLAVISVYVPSGSSSEERQQAKFRFMEAFLPHLLQLKASGREIVLCGDVNIAHKEIDIK
NWKGNLKNSGFLPEERAWIGQLFDMHGYVDVFRQLDPRPEQYTWWSNRGQAYAKNVGWRIDYHLATPKIAGTAHVCSIYK
DEKFSDHAPLSIDYNYPL

Sequences:

>Translated_258_residues
MLRIISANLNGIRSASKKGFFEWMGKQDADMVCVQELKAQAADMTEAFLAPHGYHGYFHYAEKKGYSGVGLYTRHKPERV
ITGFGNPEFDSEGRYVEVQYPHLAVISVYVPSGSSSEERQQAKFRFMEAFLPHLLQLKASGREIVLCGDVNIAHKEIDIK
NWKGNLKNSGFLPEERAWIGQLFDMHGYVDVFRQLDPRPEQYTWWSNRGQAYAKNVGWRIDYHLATPKIAGTAHVCSIYK
DEKFSDHAPLSIDYNYPL
>Mature_258_residues
MLRIISANLNGIRSASKKGFFEWMGKQDADMVCVQELKAQAADMTEAFLAPHGYHGYFHYAEKKGYSGVGLYTRHKPERV
ITGFGNPEFDSEGRYVEVQYPHLAVISVYVPSGSSSEERQQAKFRFMEAFLPHLLQLKASGREIVLCGDVNIAHKEIDIK
NWKGNLKNSGFLPEERAWIGQLFDMHGYVDVFRQLDPRPEQYTWWSNRGQAYAKNVGWRIDYHLATPKIAGTAHVCSIYK
DEKFSDHAPLSIDYNYPL

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=254, Percent_Identity=37.7952755905512, Blast_Score=170, Evalue=1e-42,
Organism=Homo sapiens, GI18375503, Length=254, Percent_Identity=37.7952755905512, Blast_Score=170, Evalue=1e-42,
Organism=Homo sapiens, GI18375501, Length=254, Percent_Identity=37.7952755905512, Blast_Score=170, Evalue=1e-42,
Organism=Homo sapiens, GI18375507, Length=310, Percent_Identity=27.4193548387097, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=30.3370786516854, Blast_Score=101, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71989536, Length=259, Percent_Identity=29.7297297297297, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI221330655, Length=255, Percent_Identity=35.2941176470588, Blast_Score=151, Evalue=5e-37,
Organism=Drosophila melanogaster, GI17136678, Length=255, Percent_Identity=35.2941176470588, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29517; Mature: 29517

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRIISANLNGIRSASKKGFFEWMGKQDADMVCVQELKAQAADMTEAFLAPHGYHGYFHY
CEEEEECCCCHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
AEKKGYSGVGLYTRHKPERVITGFGNPEFDSEGRYVEVQYPHLAVISVYVPSGSSSEERQ
HHHCCCCCCCEEECCCCCEEEECCCCCCCCCCCCEEEEECCCEEEEEEEECCCCCHHHHH
QAKFRFMEAFLPHLLQLKASGREIVLCGDVNIAHKEIDIKNWKGNLKNSGFLPEERAWIG
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHH
QLFDMHGYVDVFRQLDPRPEQYTWWSNRGQAYAKNVGWRIDYHLATPKIAGTAHVCSIYK
HHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEECCCCEEEEEEECCCCCCCCHHEEEEEC
DEKFSDHAPLSIDYNYPL
CCCCCCCCCEEEECCCCC
>Mature Secondary Structure
MLRIISANLNGIRSASKKGFFEWMGKQDADMVCVQELKAQAADMTEAFLAPHGYHGYFHY
CEEEEECCCCHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
AEKKGYSGVGLYTRHKPERVITGFGNPEFDSEGRYVEVQYPHLAVISVYVPSGSSSEERQ
HHHCCCCCCCEEECCCCCEEEECCCCCCCCCCCCEEEEECCCEEEEEEEECCCCCHHHHH
QAKFRFMEAFLPHLLQLKASGREIVLCGDVNIAHKEIDIKNWKGNLKNSGFLPEERAWIG
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHH
QLFDMHGYVDVFRQLDPRPEQYTWWSNRGQAYAKNVGWRIDYHLATPKIAGTAHVCSIYK
HHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEECCCCEEEEEEECCCCCCCCHHEEEEEC
DEKFSDHAPLSIDYNYPL
CCCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]