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The map label for this gene is mvaB [H]

Identifier: 73539859

GI number: 73539859

Start: 180898

End: 181833

Strand: Direct

Name: mvaB [H]

Synonym: Reut_A0153

Alternate gene names: 73539859

Gene position: 180898-181833 (Clockwise)

Preceding gene: 73539858

Following gene: 73539860

Centisome position: 4.75

GC content: 65.49

Gene sequence:

>936_bases
ATGAACGTGCCCAACTACGTGAAGATCGTCGAAGTCGGCCCGCGCGACGGCTTGCAGAACGAGAAGGCGATGGTGCCGAC
CGACGTCAAGGTCGCGCTGATCAACCAGCTGACCGATGCCGGCTTCGTCAATATCGAGGCCGCTTCTTTCGTCTCGCCGA
AGTGGGTGCCGCAAATGGCCGACGGCGCCGACGTGATGGCACGCATCCAGCGCCGTCCCGGCACGCTGTATTCGGTGCTC
ACGCCGAATATGAAGGGCTTCGAGGGCGCGGTGGCAGCCGGTGCCGATGAGGTTGTGATTTTCGGCGCCGCGAGCGAGGC
GTTCTCGCAACGGAACATCAACTGCTCGATCGCCGAATCGATCGCACGGTTCGAGCCGGTCGCAGCGGCCGCGAAGGAAA
AAAATATCCGCCTGCGCGGCAGCATCTCGTGCGCGCTGGGTTGCCCGTATCAGGGCGAGGTGCCGGTGAGCGCCGTGGTG
GATGTCGTGCGCCGCATGCGCGAACTGGGCTGCGACGAGATCGATATCGCCGACACCATCGGCGTGGGCACGCCCGGCAA
GGTGCAGGACGTGATGCGCGCGGTGTCGGCCGAGTTCGCGATGGACCGCCTGTCGGGGCATTTCCATGACACGTATGGCC
AGGCGCTGTCGAACATCCTCGCCAGCCTGGAAGTCGGAATCTCGATCTTCCATGCATCGGTGGCGGGCCTCGGCGGCTGC
CCGTACGCCAAGGGCGCGACCGGGAACGTGGCGACCGAAGACGTGCTGTACATGCTGCACGGGCTCGGCATCCATACCGG
CATCGATCTCGAAGCCGTGGTGCGTGCGGGGGACTATATCTCGCAGGCCATCGGCCGCGCCAACAGCTCACGCGTAGGCC
GCGCACTGCTGACCAAATGGGCGGCGACAAGCGAAGCCGCGCCGGCGTGCGCATGA

Upstream 100 bases:

>100_bases
CGCGCTGCGTGCGGGCCAGCCGATCGCCGGCGTCGTCGACCTGCAGCGCGGCTACTGAGCCGCAAGCCATCCGGTACACG
AACAGACATTGGAGACAGCG

Downstream 100 bases:

>100_bases
ATCCACGACACCAAAGAATTCAGGAGCAAGCATGAGCCAGTCCGCTGACCAGGGCAGCGCCAACCAGACCGAAGGATTGC
CGGAATCCGCGCACCGCGTT

Product: hydroxymethylglutaryl-CoA lyase

Products: NA

Alternate protein names: HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MNVPNYVKIVEVGPRDGLQNEKAMVPTDVKVALINQLTDAGFVNIEAASFVSPKWVPQMADGADVMARIQRRPGTLYSVL
TPNMKGFEGAVAAGADEVVIFGAASEAFSQRNINCSIAESIARFEPVAAAAKEKNIRLRGSISCALGCPYQGEVPVSAVV
DVVRRMRELGCDEIDIADTIGVGTPGKVQDVMRAVSAEFAMDRLSGHFHDTYGQALSNILASLEVGISIFHASVAGLGGC
PYAKGATGNVATEDVLYMLHGLGIHTGIDLEAVVRAGDYISQAIGRANSSRVGRALLTKWAATSEAAPACA

Sequences:

>Translated_311_residues
MNVPNYVKIVEVGPRDGLQNEKAMVPTDVKVALINQLTDAGFVNIEAASFVSPKWVPQMADGADVMARIQRRPGTLYSVL
TPNMKGFEGAVAAGADEVVIFGAASEAFSQRNINCSIAESIARFEPVAAAAKEKNIRLRGSISCALGCPYQGEVPVSAVV
DVVRRMRELGCDEIDIADTIGVGTPGKVQDVMRAVSAEFAMDRLSGHFHDTYGQALSNILASLEVGISIFHASVAGLGGC
PYAKGATGNVATEDVLYMLHGLGIHTGIDLEAVVRAGDYISQAIGRANSSRVGRALLTKWAATSEAAPACA
>Mature_311_residues
MNVPNYVKIVEVGPRDGLQNEKAMVPTDVKVALINQLTDAGFVNIEAASFVSPKWVPQMADGADVMARIQRRPGTLYSVL
TPNMKGFEGAVAAGADEVVIFGAASEAFSQRNINCSIAESIARFEPVAAAAKEKNIRLRGSISCALGCPYQGEVPVSAVV
DVVRRMRELGCDEIDIADTIGVGTPGKVQDVMRAVSAEFAMDRLSGHFHDTYGQALSNILASLEVGISIFHASVAGLGGC
PYAKGATGNVATEDVLYMLHGLGIHTGIDLEAVVRAGDYISQAIGRANSSRVGRALLTKWAATSEAAPACA

Specific function: Involved in the catabolism of branched amino acids such as leucine (Probable) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA lyase family [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=294, Percent_Identity=58.1632653061224, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI109150427, Length=293, Percent_Identity=55.6313993174061, Blast_Score=358, Evalue=3e-99,
Organism=Homo sapiens, GI109150422, Length=293, Percent_Identity=55.6313993174061, Blast_Score=358, Evalue=3e-99,
Organism=Homo sapiens, GI260654708, Length=134, Percent_Identity=54.4776119402985, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17510563, Length=296, Percent_Identity=48.6486486486487, Blast_Score=299, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24582381, Length=291, Percent_Identity=55.6701030927835, Blast_Score=347, Evalue=5e-96,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000138
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =4.1.3.4 [H]

Molecular weight: Translated: 32651; Mature: 32651

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS01062 HMG_COA_LYASE ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVPNYVKIVEVGPRDGLQNEKAMVPTDVKVALINQLTDAGFVNIEAASFVSPKWVPQMA
CCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEHHHHCCCCCCCCCC
DGADVMARIQRRPGTLYSVLTPNMKGFEGAVAAGADEVVIFGAASEAFSQRNINCSIAES
CCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHCCCCCEEEEECCHHHHHHCCCCHHHHHH
IARFEPVAAAAKEKNIRLRGSISCALGCPYQGEVPVSAVVDVVRRMRELGCDEIDIADTI
HHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHC
GVGTPGKVQDVMRAVSAEFAMDRLSGHFHDTYGQALSNILASLEVGISIFHASVAGLGGC
CCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PYAKGATGNVATEDVLYMLHGLGIHTGIDLEAVVRAGDYISQAIGRANSSRVGRALLTKW
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AATSEAAPACA
HCCCCCCCCCC
>Mature Secondary Structure
MNVPNYVKIVEVGPRDGLQNEKAMVPTDVKVALINQLTDAGFVNIEAASFVSPKWVPQMA
CCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEHHHHCCCCCCCCCC
DGADVMARIQRRPGTLYSVLTPNMKGFEGAVAAGADEVVIFGAASEAFSQRNINCSIAES
CCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHCCCCCEEEEECCHHHHHHCCCCHHHHHH
IARFEPVAAAAKEKNIRLRGSISCALGCPYQGEVPVSAVVDVVRRMRELGCDEIDIADTI
HHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHC
GVGTPGKVQDVMRAVSAEFAMDRLSGHFHDTYGQALSNILASLEVGISIFHASVAGLGGC
CCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PYAKGATGNVATEDVLYMLHGLGIHTGIDLEAVVRAGDYISQAIGRANSSRVGRALLTKW
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AATSEAAPACA
HCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2687236; 2656635; 1637819 [H]