Click here to switch to the map view.

The map label for this gene is ghrA [H]

Identifier: 73539858

GI number: 73539858

Start: 179914

End: 180855

Strand: Direct

Name: ghrA [H]

Synonym: Reut_A0152

Alternate gene names: 73539858

Gene position: 179914-180855 (Clockwise)

Preceding gene: 73539857

Following gene: 73539859

Centisome position: 4.73

GC content: 68.47

Gene sequence:

>942_bases
ATGAAGTTGCAGTTGTACGTGCCCGATGGCCGCTATGAACCCTGGATCGAAGGCTTCGCGCAGGCGCTGCCCGAAGCGCA
GTGCGTCACCTGGGAAGATAGCCGCGGCGAACCGGCCGACTACGCCGTGGTCTGGCGTCCTCCCGTGGAAATGCTGCGCG
GCCGTACCGACCTGAAGGCGGTGTTCAACCTCGGCGCCGGCGTGGACGGCATCTTGCGCCTGCGCGACCAGGATCCGGAT
GCGCTGCCCGCCGGCGTGCCGATCGTGCGCCTGGACGACGCCGGCATGGCCGCCCAGATGGTCGAATACGTCACCCATGC
GGTGCTGCGCTATTTCCGCCGGTTGGACGACTATGCGGCGCAGCAGCAGGCCGGCAACTGGAAGTTCCTGAAGCCGCACC
GCCGTGCGGACTTCACCATCGGCGTGATGGGCGTTGGCGCGCTCGGCACGCATATCGCGCGCACGCTGGCCGGGTTCGGG
TTTCCGGTGCGCGGCTGGAGCCGCACCGCGCGCGCGATCGAGGGCGTGGTGGGCTTCCATGGCGATGCCGGCCGCGTGCC
GTTCCTCGACGGCTTGCGCGTGCTGGTCAATGTGCTGCCGCTGACGCCTGAGACCGAAAACATCCTGAACGGCGAACTGT
TCGGCAAGCTGGCGAAGGGCGCCTACGTGATCAATGTCGCGCGTGGCCAGCATCTCGCGGAGGACGATCTGCTCGCCGCC
GTGCAGGCAGGACAGATCGCCGGCGCGACACTCGACGTATTCCGCACCGAGCCGCTGCCGGCGGACCACCCGTTCTGGCA
GGAACCGCGCATCACCATCACGCCGCATATCTCGGCGCTGACGCTGCGCGAGGACAGCATCGCGCAGATTGCCGGCAAGA
TCCGCGCGCTGCGTGCGGGCCAGCCGATCGCCGGCGTCGTCGACCTGCAGCGCGGCTACTGA

Upstream 100 bases:

>100_bases
GCCGCCGGCGGGAGCGGGGCGATTGCCGGCCACCGGCCGGCACCGTCGCGCGACCTCTGCGAAAATGCAGGCATCCCGCC
ACAGAAGACCCGGAGTCCCC

Downstream 100 bases:

>100_bases
GCCGCAAGCCATCCGGTACACGAACAGACATTGGAGACAGCGATGAACGTGCCCAACTACGTGAAGATCGTCGAAGTCGG
CCCGCGCGACGGCTTGCAGA

Product: 6-phosphogluconate dehydrogenase, NAD-binding:D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKLQLYVPDGRYEPWIEGFAQALPEAQCVTWEDSRGEPADYAVVWRPPVEMLRGRTDLKAVFNLGAGVDGILRLRDQDPD
ALPAGVPIVRLDDAGMAAQMVEYVTHAVLRYFRRLDDYAAQQQAGNWKFLKPHRRADFTIGVMGVGALGTHIARTLAGFG
FPVRGWSRTARAIEGVVGFHGDAGRVPFLDGLRVLVNVLPLTPETENILNGELFGKLAKGAYVINVARGQHLAEDDLLAA
VQAGQIAGATLDVFRTEPLPADHPFWQEPRITITPHISALTLREDSIAQIAGKIRALRAGQPIAGVVDLQRGY

Sequences:

>Translated_313_residues
MKLQLYVPDGRYEPWIEGFAQALPEAQCVTWEDSRGEPADYAVVWRPPVEMLRGRTDLKAVFNLGAGVDGILRLRDQDPD
ALPAGVPIVRLDDAGMAAQMVEYVTHAVLRYFRRLDDYAAQQQAGNWKFLKPHRRADFTIGVMGVGALGTHIARTLAGFG
FPVRGWSRTARAIEGVVGFHGDAGRVPFLDGLRVLVNVLPLTPETENILNGELFGKLAKGAYVINVARGQHLAEDDLLAA
VQAGQIAGATLDVFRTEPLPADHPFWQEPRITITPHISALTLREDSIAQIAGKIRALRAGQPIAGVVDLQRGY
>Mature_313_residues
MKLQLYVPDGRYEPWIEGFAQALPEAQCVTWEDSRGEPADYAVVWRPPVEMLRGRTDLKAVFNLGAGVDGILRLRDQDPD
ALPAGVPIVRLDDAGMAAQMVEYVTHAVLRYFRRLDDYAAQQQAGNWKFLKPHRRADFTIGVMGVGALGTHIARTLAGFG
FPVRGWSRTARAIEGVVGFHGDAGRVPFLDGLRVLVNVLPLTPETENILNGELFGKLAKGAYVINVARGQHLAEDDLLAA
VQAGQIAGATLDVFRTEPLPADHPFWQEPRITITPHISALTLREDSIAQIAGKIRALRAGQPIAGVVDLQRGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=216, Percent_Identity=30.5555555555556, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI23308577, Length=270, Percent_Identity=28.5185185185185, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI87081824, Length=313, Percent_Identity=48.2428115015974, Blast_Score=305, Evalue=3e-84,
Organism=Escherichia coli, GI87082289, Length=244, Percent_Identity=29.5081967213115, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17532191, Length=251, Percent_Identity=29.8804780876494, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320925, Length=201, Percent_Identity=31.3432835820896, Blast_Score=70, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6322116, Length=202, Percent_Identity=30.1980198019802, Blast_Score=69, Evalue=6e-13,
Organism=Drosophila melanogaster, GI28574286, Length=257, Percent_Identity=27.2373540856031, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI45552429, Length=254, Percent_Identity=27.5590551181102, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24585514, Length=183, Percent_Identity=30.6010928961749, Blast_Score=78, Evalue=8e-15,
Organism=Drosophila melanogaster, GI28574282, Length=183, Percent_Identity=30.6010928961749, Blast_Score=78, Evalue=8e-15,
Organism=Drosophila melanogaster, GI28574284, Length=254, Percent_Identity=27.5590551181102, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI45551003, Length=254, Percent_Identity=27.5590551181102, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28571528, Length=207, Percent_Identity=30.4347826086957, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24585516, Length=162, Percent_Identity=32.7160493827161, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19921140, Length=235, Percent_Identity=27.2340425531915, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 34257; Mature: 34257

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQLYVPDGRYEPWIEGFAQALPEAQCVTWEDSRGEPADYAVVWRPPVEMLRGRTDLKA
CEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCCHHHHCCCHHHHH
VFNLGAGVDGILRLRDQDPDALPAGVPIVRLDDAGMAAQMVEYVTHAVLRYFRRLDDYAA
HHHCCCCCCEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QQQAGNWKFLKPHRRADFTIGVMGVGALGTHIARTLAGFGFPVRGWSRTARAIEGVVGFH
HHHCCCCCEECCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
GDAGRVPFLDGLRVLVNVLPLTPETENILNGELFGKLAKGAYVINVARGQHLAEDDLLAA
CCCCCCCHHHHHHHHHHHHCCCCCHHHHHCHHHHHHHHCCEEEEEECCCCCCCHHHHHHH
VQAGQIAGATLDVFRTEPLPADHPFWQEPRITITPHISALTLREDSIAQIAGKIRALRAG
HHCCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCCEEEEECCHHHHHHHHHHHHHHCC
QPIAGVVDLQRGY
CCCEEEEECCCCC
>Mature Secondary Structure
MKLQLYVPDGRYEPWIEGFAQALPEAQCVTWEDSRGEPADYAVVWRPPVEMLRGRTDLKA
CEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCCHHHHCCCHHHHH
VFNLGAGVDGILRLRDQDPDALPAGVPIVRLDDAGMAAQMVEYVTHAVLRYFRRLDDYAA
HHHCCCCCCEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QQQAGNWKFLKPHRRADFTIGVMGVGALGTHIARTLAGFGFPVRGWSRTARAIEGVVGFH
HHHCCCCCEECCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
GDAGRVPFLDGLRVLVNVLPLTPETENILNGELFGKLAKGAYVINVARGQHLAEDDLLAA
CCCCCCCHHHHHHHHHHHHCCCCCHHHHHCHHHHHHHHCCEEEEEECCCCCCCHHHHHHH
VQAGQIAGATLDVFRTEPLPADHPFWQEPRITITPHISALTLREDSIAQIAGKIRALRAG
HHCCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCCEEEEECCHHHHHHHHHHHHHHCC
QPIAGVVDLQRGY
CCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA