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The map label for this gene is bioD [H]

Identifier: 73539855

GI number: 73539855

Start: 175925

End: 176671

Strand: Direct

Name: bioD [H]

Synonym: Reut_A0149

Alternate gene names: 73539855

Gene position: 175925-176671 (Clockwise)

Preceding gene: 73539854

Following gene: 73539856

Centisome position: 4.62

GC content: 69.08

Gene sequence:

>747_bases
ATGAACCGCATAAACCAGCCCGCCAGGTTCCAGTGCTTCGTCACCGGCACCGACACCGGCGTCGGCAAGACCCACGCGAG
CGCCACGCTGCTGCATGCGCTGCACGCGGCCGGCTATGCCGCCGTCGGCATGAAGCCCGTGGCCAGCGGCAGCGAATGGC
GCGTCGATCACTGGCACAACGATGACGTGGCCGCGCTGCGTGCCGCCAGCTCCGTCGCGGTGCCGCTGGGACAGACCTGC
CCGTTCTTGCTGCGCACGCCATGCTCGCCGCATCTGGCTGCGGCGCAGGAAGGCGTGCGCATCACGCGTGCGCCGATCCG
CCATGCATTCGACGCGCTGCGCGAGCAGGCCGATGCCGTCGTCGTTGAAGGCGTCGGCGGCTTCCATGTGCCGCTCGATA
CCGGCGCGGTGCGCTGGAGCACGGCGGACCTTGCCGAGATGCTGGCGCTGCCAGTCGTCATGGTGGTGGGCATCCGCCTC
GGTTGCCTGAATCACGCCGTGCTGACCGCCGAAGCCATCCGCGCACGCGGCCTGACACTGGCTGGCTGGATCGCCAACCG
TGTCGACCCGGACATGTTGCTGGCTGACGAGAACATCGCCACGCTGCATGACGCGCTCGACGCACCTTGCCTGGGCGAGC
TGCCGTGGCAACTGGCGCCGGCCGATGCCGCTGCCCGTCTCGACCTCGCGCCGCTGTTTGCCCATTCCCTCCAATACCGA
GCCGAGGCCACAAGCCTCGCCGCATAA

Upstream 100 bases:

>100_bases
TGCGCATCACGTTGTCGGCCACGCATACGGCGGCGGACGTGCAGCGCCTCGTTGGCGTGCTGGCCGACGCGGTGCCCCCC
GAAGCGAAGCGGGAGGCCGC

Downstream 100 bases:

>100_bases
AACCAGAACCTCACCATGAACCAAGCCGCTCAAACCGTCGCCACCATCTCCGCCGAAGCGCTGCGCCAGACCGCGCGCAA
CACCCATGCATTGCCGGAAG

Product: dethiobiotin synthase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MNRINQPARFQCFVTGTDTGVGKTHASATLLHALHAAGYAAVGMKPVASGSEWRVDHWHNDDVAALRAASSVAVPLGQTC
PFLLRTPCSPHLAAAQEGVRITRAPIRHAFDALREQADAVVVEGVGGFHVPLDTGAVRWSTADLAEMLALPVVMVVGIRL
GCLNHAVLTAEAIRARGLTLAGWIANRVDPDMLLADENIATLHDALDAPCLGELPWQLAPADAAARLDLAPLFAHSLQYR
AEATSLAA

Sequences:

>Translated_248_residues
MNRINQPARFQCFVTGTDTGVGKTHASATLLHALHAAGYAAVGMKPVASGSEWRVDHWHNDDVAALRAASSVAVPLGQTC
PFLLRTPCSPHLAAAQEGVRITRAPIRHAFDALREQADAVVVEGVGGFHVPLDTGAVRWSTADLAEMLALPVVMVVGIRL
GCLNHAVLTAEAIRARGLTLAGWIANRVDPDMLLADENIATLHDALDAPCLGELPWQLAPADAAARLDLAPLFAHSLQYR
AEATSLAA
>Mature_248_residues
MNRINQPARFQCFVTGTDTGVGKTHASATLLHALHAAGYAAVGMKPVASGSEWRVDHWHNDDVAALRAASSVAVPLGQTC
PFLLRTPCSPHLAAAQEGVRITRAPIRHAFDALREQADAVVVEGVGGFHVPLDTGAVRWSTADLAEMLALPVVMVVGIRL
GCLNHAVLTAEAIRARGLTLAGWIANRVDPDMLLADENIATLHDALDAPCLGELPWQLAPADAAARLDLAPLFAHSLQYR
AEATSLAA

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1786995, Length=205, Percent_Identity=48.2926829268293, Blast_Score=174, Evalue=6e-45,
Organism=Escherichia coli, GI87081944, Length=210, Percent_Identity=39.5238095238095, Blast_Score=132, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 26202; Mature: 26202

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRINQPARFQCFVTGTDTGVGKTHASATLLHALHAAGYAAVGMKPVASGSEWRVDHWHN
CCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCCCEEECCCCC
DDVAALRAASSVAVPLGQTCPFLLRTPCSPHLAAAQEGVRITRAPIRHAFDALREQADAV
CCHHHHHHHCCCCCCCCCCCCHHHCCCCCCCHHHHHCCCEEEHHHHHHHHHHHHHHCCEE
VVEGVGGFHVPLDTGAVRWSTADLAEMLALPVVMVVGIRLGCLNHAVLTAEAIRARGLTL
EEECCCCEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
AGWIANRVDPDMLLADENIATLHDALDAPCLGELPWQLAPADAAARLDLAPLFAHSLQYR
EHHHHHCCCCCEEEECCCHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHH
AEATSLAA
HHHHHCCC
>Mature Secondary Structure
MNRINQPARFQCFVTGTDTGVGKTHASATLLHALHAAGYAAVGMKPVASGSEWRVDHWHN
CCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCCCEEECCCCC
DDVAALRAASSVAVPLGQTCPFLLRTPCSPHLAAAQEGVRITRAPIRHAFDALREQADAV
CCHHHHHHHCCCCCCCCCCCCHHHCCCCCCCHHHHHCCCEEEHHHHHHHHHHHHHHCCEE
VVEGVGGFHVPLDTGAVRWSTADLAEMLALPVVMVVGIRLGCLNHAVLTAEAIRARGLTL
EEECCCCEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
AGWIANRVDPDMLLADENIATLHDALDAPCLGELPWQLAPADAAARLDLAPLFAHSLQYR
EHHHHHCCCCCEEEECCCHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHH
AEATSLAA
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA