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The map label for this gene is bioB [H]

Identifier: 73539856

GI number: 73539856

Start: 176687

End: 177715

Strand: Direct

Name: bioB [H]

Synonym: Reut_A0150

Alternate gene names: 73539856

Gene position: 176687-177715 (Clockwise)

Preceding gene: 73539855

Following gene: 73539857

Centisome position: 4.64

GC content: 66.08

Gene sequence:

>1029_bases
ATGAACCAAGCCGCTCAAACCGTCGCCACCATCTCCGCCGAAGCGCTGCGCCAGACCGCGCGCAACACCCATGCATTGCC
GGAAGACGCCCGCTGGCGCGTCGACGACGTGGCCGCGCTGTTCGCGCTGCCGTTCAACGACCTGCTGTTCCGCGCGCAGC
AGGTGCATCGCGAGAACTTCGACGCCAACACCGTGCAGCTTTCCACGCTGCTGTCGATCAAGACCGGCGGCTGCGAAGAG
GATTGCGGCTACTGCCCGCAAAGCGCGCACCACGACGCCGGCGTCAAGGCCGAAAAGCTGATGGAACTCGATGAAGTGCT
CGAAGCCGCCCGCGCTGCCAAGGCCAACGGCGCTACGCGCTTTTGCATGGGAGCCGCCTGGCGCAGCCCGAAGGACCGCC
ACCTGGAGCCGGTCATGGACATGGTGCGCGAGGTCAAGGCCATGGGCCTGGAAACCTGCGTGACGCTCGGCATGCTGAAG
GCCGAGCAGGCGCAGCAGCTCAAGGACGCGGGCCTGGATTATTACAACCACAACCTCGATACCTCGCCCGAGTTCTACGG
CAAGATCATCACCACGCGCACGTACCAGGATCGTCTCGATACGATCGGCCATGTGCGCGACGCCGGCATCAACGTGTGCT
GCGGCGGCATCGTCGGCATGGGGGAATCACGCGAGGCGCGTGCCGGGCTGATCGCGCAGCTCGCCAATATGGATCCGTAT
CCGGAATCGGTGCCCATCAACAACCTGGTGCAGGTCGAAGGCACGCCGCTGGCCGGCACCGAGGCGCTGGACCCGTTCGA
GTTTGTCCGTACGATCGCCGTGGCCCGCATCACCATGCCGGGCGCGATGGTGCGCCTCTCCGCCGGCCGCGAGGCGATGG
ACGAGGCGCTGCAGGCGTTGTGCTTCATGGCGGGCGCCAACTCCATCTTCTACGGCGAAAAGCTGCTGACCACGGGCAAT
CCGCAGGCTGACCGCGACCGCGCGCTGCTGGCGCGACTCGACATCCGTGCGGAAGGCTACGCAGGCTAA

Upstream 100 bases:

>100_bases
CGATGCCGCTGCCCGTCTCGACCTCGCGCCGCTGTTTGCCCATTCCCTCCAATACCGAGCCGAGGCCACAAGCCTCGCCG
CATAAAACCAGAACCTCACC

Downstream 100 bases:

>100_bases
TAGTTAAGAAATCTTCACGTGAAGGGCTCCCCCGCCGTAAGATCGCCATAACGGGGTTAAAAGGAGGGAGCTCATGTTCA
GCAAGATCCTGATCGCCAAC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MNQAAQTVATISAEALRQTARNTHALPEDARWRVDDVAALFALPFNDLLFRAQQVHRENFDANTVQLSTLLSIKTGGCEE
DCGYCPQSAHHDAGVKAEKLMELDEVLEAARAAKANGATRFCMGAAWRSPKDRHLEPVMDMVREVKAMGLETCVTLGMLK
AEQAQQLKDAGLDYYNHNLDTSPEFYGKIITTRTYQDRLDTIGHVRDAGINVCCGGIVGMGESREARAGLIAQLANMDPY
PESVPINNLVQVEGTPLAGTEALDPFEFVRTIAVARITMPGAMVRLSAGREAMDEALQALCFMAGANSIFYGEKLLTTGN
PQADRDRALLARLDIRAEGYAG

Sequences:

>Translated_342_residues
MNQAAQTVATISAEALRQTARNTHALPEDARWRVDDVAALFALPFNDLLFRAQQVHRENFDANTVQLSTLLSIKTGGCEE
DCGYCPQSAHHDAGVKAEKLMELDEVLEAARAAKANGATRFCMGAAWRSPKDRHLEPVMDMVREVKAMGLETCVTLGMLK
AEQAQQLKDAGLDYYNHNLDTSPEFYGKIITTRTYQDRLDTIGHVRDAGINVCCGGIVGMGESREARAGLIAQLANMDPY
PESVPINNLVQVEGTPLAGTEALDPFEFVRTIAVARITMPGAMVRLSAGREAMDEALQALCFMAGANSIFYGEKLLTTGN
PQADRDRALLARLDIRAEGYAG
>Mature_342_residues
MNQAAQTVATISAEALRQTARNTHALPEDARWRVDDVAALFALPFNDLLFRAQQVHRENFDANTVQLSTLLSIKTGGCEE
DCGYCPQSAHHDAGVKAEKLMELDEVLEAARAAKANGATRFCMGAAWRSPKDRHLEPVMDMVREVKAMGLETCVTLGMLK
AEQAQQLKDAGLDYYNHNLDTSPEFYGKIITTRTYQDRLDTIGHVRDAGINVCCGGIVGMGESREARAGLIAQLANMDPY
PESVPINNLVQVEGTPLAGTEALDPFEFVRTIAVARITMPGAMVRLSAGREAMDEALQALCFMAGANSIFYGEKLLTTGN
PQADRDRALLARLDIRAEGYAG

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=311, Percent_Identity=63.9871382636656, Blast_Score=420, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6321725, Length=314, Percent_Identity=52.2292993630573, Blast_Score=337, Evalue=2e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 37250; Mature: 37250

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQAAQTVATISAEALRQTARNTHALPEDARWRVDDVAALFALPFNDLLFRAQQVHRENF
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCC
DANTVQLSTLLSIKTGGCEEDCGYCPQSAHHDAGVKAEKLMELDEVLEAARAAKANGATR
CCCCEEEEHHEEECCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHH
FCMGAAWRSPKDRHLEPVMDMVREVKAMGLETCVTLGMLKAEQAQQLKDAGLDYYNHNLD
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCC
TSPEFYGKIITTRTYQDRLDTIGHVRDAGINVCCGGIVGMGESREARAGLIAQLANMDPY
CCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCC
PESVPINNLVQVEGTPLAGTEALDPFEFVRTIAVARITMPGAMVRLSAGREAMDEALQAL
CCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
CFMAGANSIFYGEKLLTTGNPQADRDRALLARLDIRAEGYAG
HHHHCCCCEEECCEEEECCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNQAAQTVATISAEALRQTARNTHALPEDARWRVDDVAALFALPFNDLLFRAQQVHRENF
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCC
DANTVQLSTLLSIKTGGCEEDCGYCPQSAHHDAGVKAEKLMELDEVLEAARAAKANGATR
CCCCEEEEHHEEECCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHH
FCMGAAWRSPKDRHLEPVMDMVREVKAMGLETCVTLGMLKAEQAQQLKDAGLDYYNHNLD
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCC
TSPEFYGKIITTRTYQDRLDTIGHVRDAGINVCCGGIVGMGESREARAGLIAQLANMDPY
CCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCC
PESVPINNLVQVEGTPLAGTEALDPFEFVRTIAVARITMPGAMVRLSAGREAMDEALQAL
CCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
CFMAGANSIFYGEKLLTTGNPQADRDRALLARLDIRAEGYAG
HHHHCCCCEEECCEEEECCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA