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The map label for this gene is bioF

Identifier: 73539854

GI number: 73539854

Start: 174702

End: 175928

Strand: Direct

Name: bioF

Synonym: Reut_A0148

Alternate gene names: 73539854

Gene position: 174702-175928 (Clockwise)

Preceding gene: 73539853

Following gene: 73539855

Centisome position: 4.59

GC content: 69.68

Gene sequence:

>1227_bases
ATGCTGCTTGAACAATTGCGCCGCGCCGCGGACGAACGCCAGGCCCGCGCGCTCACGCGCCGGCGCCGCATTGCGCATAC
CGCATGCGCGCCGCACCAGGCCGTGGGCCCGGTGGATGCACCGGCGCCGCAGCCGTTGCTGACGTTCTGCAGCAACGACT
ACATGGGACTCGCAAGCCATCCCGATGTCGTTGCCGCGCTAGCCGACGGAGCACGTCTCTACGGCGCCGGCAGCGGCGCT
TCGCATCTTGTCAGCGGGCATTCGCTTGCGCATGCGCGGCTCGAAGAGGAACTCGCGCGCTGGTTCGCGCCGCACATCCC
GCAAGCGCGCACGCTGTACTTCTGCACCGGCTACATGGCCAACATGGCCGTGCTGACGGCGCTGGGCACCGCGGGCGCGA
CGCTGTTCTGCGAAACGCTGAACCATGCCTCGCTGATCGACGGCGCACGCCTTGCGCGCGCCGATGTGCAGCGCTACCCG
CACTGCGACGCCGCCGCACTGGACGCGCTGCTCGCGGCCAGCACCAGCCCGCTCAAGCTGATCGTCACCGACAGCGTGTT
CAGCATGGACGGCGACGTGGCGCCGCTGGCGGAATTGCTTGCGCTGGCCGAACGCCATGACGCGTGGATCATCGTCGACG
ATGCGCATGGCTTCGGCGTGCTCGGCGACGATGGCCATGGCGTACTGCAGGCATTGGGGCTGAACTCGGAGCGCTTCATC
TACATCGGCACGCTCGGCAAGGCCGCCGGCGTGGCGGGCGCGTTCGTCGCCGCGCACGAGACCATCATCGAACATCTGGT
CAACACCGCGCGGCCCTACATCTACACCACGGCTGCCCCGCCGGCGGTTGCGCATGCGCTGATGGCAAGCCTTGCCATCA
TTGAGGGCGAGGAGGGCCGGCAGCGCCGCGCCAACCTGAACCAGCTGATCGCCGACCTCCGCGCAGGCCTCGCGCCGCTG
GCCGCCGCAGCCGGCTGGGAACTCGGCGACAGTGCCACCGCTGTGCAGCCTCTGATTGTCGGGGACAACGCTGTCTCGCT
GGCATTGTCCGCGCGCCTCGAAGCAGAAGGCATCCGCGTCGGCGCGATCCGGCCGCCAACCGTGCCCGAGGGAACCGCGC
GGCTGCGCATCACGTTGTCGGCCACGCATACGGCGGCGGACGTGCAGCGCCTCGTTGGCGTGCTGGCCGACGCGGTGCCC
CCCGAAGCGAAGCGGGAGGCCGCATGA

Upstream 100 bases:

>100_bases
TACGTATTCGATGCCGAGCAGGCGCGCTGGCTCGCCACGCAACTGCAGGCCACGCTGGACGATGTGCTCGGCAACGCCGA
GGCTGACAAGGAGGTACGCC

Downstream 100 bases:

>100_bases
ACCGCATAAACCAGCCCGCCAGGTTCCAGTGCTTCGTCACCGGCACCGACACCGGCGTCGGCAAGACCCACGCGAGCGCC
ACGCTGCTGCATGCGCTGCA

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MLLEQLRRAADERQARALTRRRRIAHTACAPHQAVGPVDAPAPQPLLTFCSNDYMGLASHPDVVAALADGARLYGAGSGA
SHLVSGHSLAHARLEEELARWFAPHIPQARTLYFCTGYMANMAVLTALGTAGATLFCETLNHASLIDGARLARADVQRYP
HCDAAALDALLAASTSPLKLIVTDSVFSMDGDVAPLAELLALAERHDAWIIVDDAHGFGVLGDDGHGVLQALGLNSERFI
YIGTLGKAAGVAGAFVAAHETIIEHLVNTARPYIYTTAAPPAVAHALMASLAIIEGEEGRQRRANLNQLIADLRAGLAPL
AAAAGWELGDSATAVQPLIVGDNAVSLALSARLEAEGIRVGAIRPPTVPEGTARLRITLSATHTAADVQRLVGVLADAVP
PEAKREAA

Sequences:

>Translated_408_residues
MLLEQLRRAADERQARALTRRRRIAHTACAPHQAVGPVDAPAPQPLLTFCSNDYMGLASHPDVVAALADGARLYGAGSGA
SHLVSGHSLAHARLEEELARWFAPHIPQARTLYFCTGYMANMAVLTALGTAGATLFCETLNHASLIDGARLARADVQRYP
HCDAAALDALLAASTSPLKLIVTDSVFSMDGDVAPLAELLALAERHDAWIIVDDAHGFGVLGDDGHGVLQALGLNSERFI
YIGTLGKAAGVAGAFVAAHETIIEHLVNTARPYIYTTAAPPAVAHALMASLAIIEGEEGRQRRANLNQLIADLRAGLAPL
AAAAGWELGDSATAVQPLIVGDNAVSLALSARLEAEGIRVGAIRPPTVPEGTARLRITLSATHTAADVQRLVGVLADAVP
PEAKREAA
>Mature_408_residues
MLLEQLRRAADERQARALTRRRRIAHTACAPHQAVGPVDAPAPQPLLTFCSNDYMGLASHPDVVAALADGARLYGAGSGA
SHLVSGHSLAHARLEEELARWFAPHIPQARTLYFCTGYMANMAVLTALGTAGATLFCETLNHASLIDGARLARADVQRYP
HCDAAALDALLAASTSPLKLIVTDSVFSMDGDVAPLAELLALAERHDAWIIVDDAHGFGVLGDDGHGVLQALGLNSERFI
YIGTLGKAAGVAGAFVAAHETIIEHLVNTARPYIYTTAAPPAVAHALMASLAIIEGEEGRQRRANLNQLIADLRAGLAPL
AAAAGWELGDSATAVQPLIVGDNAVSLALSARLEAEGIRVGAIRPPTVPEGTARLRITLSATHTAADVQRLVGVLADAVP
PEAKREAA

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily

Homologues:

Organism=Homo sapiens, GI7657118, Length=392, Percent_Identity=33.4183673469388, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI284448556, Length=408, Percent_Identity=32.3529411764706, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI83977442, Length=351, Percent_Identity=31.6239316239316, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI83977440, Length=358, Percent_Identity=31.2849162011173, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI83977444, Length=358, Percent_Identity=31.2849162011173, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI4502025, Length=340, Percent_Identity=32.9411764705882, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI40316939, Length=340, Percent_Identity=32.9411764705882, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI4758668, Length=353, Percent_Identity=27.7620396600567, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI119220554, Length=354, Percent_Identity=26.271186440678, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI5454084, Length=265, Percent_Identity=25.2830188679245, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1786993, Length=349, Percent_Identity=45.2722063037249, Blast_Score=271, Evalue=4e-74,
Organism=Escherichia coli, GI1790046, Length=397, Percent_Identity=32.7455919395466, Blast_Score=198, Evalue=6e-52,
Organism=Caenorhabditis elegans, GI71994529, Length=362, Percent_Identity=30.3867403314917, Blast_Score=191, Evalue=7e-49,
Organism=Caenorhabditis elegans, GI32566772, Length=354, Percent_Identity=28.2485875706215, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17560914, Length=319, Percent_Identity=24.1379310344828, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17560912, Length=319, Percent_Identity=24.1379310344828, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71982625, Length=328, Percent_Identity=21.3414634146341, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71982617, Length=329, Percent_Identity=21.2765957446809, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320438, Length=381, Percent_Identity=30.4461942257218, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320267, Length=386, Percent_Identity=26.6839378238342, Blast_Score=119, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6323954, Length=266, Percent_Identity=24.812030075188, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24662918, Length=358, Percent_Identity=32.6815642458101, Blast_Score=202, Evalue=5e-52,
Organism=Drosophila melanogaster, GI17137420, Length=393, Percent_Identity=31.0432569974555, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI17136286, Length=355, Percent_Identity=27.6056338028169, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24653280, Length=264, Percent_Identity=23.4848484848485, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24653276, Length=264, Percent_Identity=23.4848484848485, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24653278, Length=264, Percent_Identity=23.4848484848485, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOF_CUPPJ (Q477A3)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_294374.1
- HSSP:   P12998
- ProteinModelPortal:   Q477A3
- GeneID:   3610714
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A0148
- NMPDR:   fig|264198.3.peg.660
- HOGENOM:   HBG649839
- OMA:   ATVMAMS
- ProtClustDB:   PRK05958
- BioCyc:   REUT264198:REUT_A0148-MONOMER
- HAMAP:   MF_01693
- InterPro:   IPR022834
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR00858

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.47

Molecular weight: Translated: 42691; Mature: 42691

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 20-20 BINDING 142-142 BINDING 188-188 BINDING 367-367

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLEQLRRAADERQARALTRRRRIAHTACAPHQAVGPVDAPAPQPLLTFCSNDYMGLASH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCC
PDVVAALADGARLYGAGSGASHLVSGHSLAHARLEEELARWFAPHIPQARTLYFCTGYMA
CHHHHHHHCCCEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHH
NMAVLTALGTAGATLFCETLNHASLIDGARLARADVQRYPHCDAAALDALLAASTSPLKL
HHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEE
IVTDSVFSMDGDVAPLAELLALAERHDAWIIVDDAHGFGVLGDDGHGVLQALGLNSERFI
EEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCHHHHHHCCCCCCEE
YIGTLGKAAGVAGAFVAAHETIIEHLVNTARPYIYTTAAPPAVAHALMASLAIIEGEEGR
EEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHEECCCCH
QRRANLNQLIADLRAGLAPLAAAAGWELGDSATAVQPLIVGDNAVSLALSARLEAEGIRV
HHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCEEEECCCCEEEHEEHEECCCCEEE
GAIRPPTVPEGTARLRITLSATHTAADVQRLVGVLADAVPPEAKREAA
CCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCCHHCCCC
>Mature Secondary Structure
MLLEQLRRAADERQARALTRRRRIAHTACAPHQAVGPVDAPAPQPLLTFCSNDYMGLASH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCC
PDVVAALADGARLYGAGSGASHLVSGHSLAHARLEEELARWFAPHIPQARTLYFCTGYMA
CHHHHHHHCCCEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHH
NMAVLTALGTAGATLFCETLNHASLIDGARLARADVQRYPHCDAAALDALLAASTSPLKL
HHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEE
IVTDSVFSMDGDVAPLAELLALAERHDAWIIVDDAHGFGVLGDDGHGVLQALGLNSERFI
EEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCHHHHHHCCCCCCEE
YIGTLGKAAGVAGAFVAAHETIIEHLVNTARPYIYTTAAPPAVAHALMASLAIIEGEEGR
EEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHEECCCCH
QRRANLNQLIADLRAGLAPLAAAAGWELGDSATAVQPLIVGDNAVSLALSARLEAEGIRV
HHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCEEEECCCCEEEHEEHEECCCCEEE
GAIRPPTVPEGTARLRITLSATHTAADVQRLVGVLADAVPPEAKREAA
CCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA