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The map label for this gene is aceK

Identifier: 73539842

GI number: 73539842

Start: 162047

End: 163873

Strand: Direct

Name: aceK

Synonym: Reut_A0136

Alternate gene names: 73539842

Gene position: 162047-163873 (Clockwise)

Preceding gene: 73539841

Following gene: 73539843

Centisome position: 4.26

GC content: 60.65

Gene sequence:

>1827_bases
ATGTCCCACTTCCCCAAACTGCTCTCTTCGCAGATTGCCTTCGACGTGGCGCGGACCATGCTGGACGGGTTCGACAAGCA
TTACCGGCTATTCCGCGAGGTCAGCCGCCAGGCCAAGGTGAAATTCGAGGCCGGCGACTGGCATAGCCTGCAGCAGATGC
AACGCGACCGCATCGCGTTCTACAACGAGCGCGTGCGCGAAACCAGCGTGATCCTCGAGGACGAGTACGACGCCGAGAAT
ATCGAGGACGAGATCTGGCAGCAGATCAAGCTGCACTACATCGGCCTGTTGACCAATCACCACCAGCCGGAGCTGGCCGA
GACGTTCTTCAATTCGGTCTGCACGCGCCTGCTGCACCGCTCTTACTTCAACAACGACTTCATCTTCGTGCGCCCGGCGA
TCTCGACCGAGTACATCGAGAACGAGGAGTCGCCGACCAGGCCCACGTTCCGCGCCTACTACCCCGGCAGCCGGCAGGGC
ATGGCCGAGTGCTTCGAGCGGATCGTGCACAACTTCCAGCTGGAAACGCCGTTCGAAGACCTGCGCCGCGACGTCGGCCA
TGTCGTGCGGGGCGTGGAGGAGCACTTCGGCGACTTCCGCATCGCGCCGAATTTCCAGATCCACGTGCTGTCGTCGCTGT
TCTTCCGCAACAAGACGGCCTTCATCATCGGCCGCATCGTGAACGCCGATCGCACCTATCCGCTGGCGATTCCGATCGTG
CACGGTCCGTCGGGCAAGCTGACACTGGACGCCGTGCTGCTCAAGAAGGTGCAGGTGCTGATCCTGTTCTCGTTCACGCA
CTCGTACTTCATGGTCGACATGGAAATCCCATCGGCCTACGTGACCTTCCTGCGCGACATCATGCCGCGCAAGCCGCGCG
CGGAGATCTACACCTCGCTCGGTTTGCAGAAGCAGGGCAAAAACCTGTTCTACCGCGATTTCCTCCATCACCTGCAGCAT
TCGTCGGACAAGTTCATCAGCGCGCCGGGCATCAAGGGGCTGGTAATGCTGGTGTTCACGCTGCCGTCGTATCCGTATGT
GTTCAAGGTCATCAAGGATTTCTACCCGGCGCCGAAGGAGACCACGCGCGAGCTGATCAAGTCCAAGTACCAGCTCGTCA
AGCAGCACGACCGCGTCGGCCGCATGGCCGACACGCTGGAATACTCCAACGTGGCCTTTCCGCTGTCGCGTTTCGACGAG
GACCTCGTGCGCGAGCTGGAACACCACGCGCCGTCGATGATCGAATACCAGCGCGGCAAGGACGGCGGCGAGGAGATCGT
GGTACGCCACGTCTACATCGAGCGCCGCATGACGCCGCTGAACATCTGGCTGCAGGAAGGCACCGACGAGCAACTCGAGC
ACGGCATCATCGAATACGGCAATGCCATCAAGGAGCTGATCGCCGCTAACATCTTCCCGGGCGACATGCTGTACAAGAAT
TTCGGCGTAACGCGCCACGGCCGCGTGGTGTTCTACGACTACGACGAGATCGAGTACTTCACCGACTGCAATGTGCGCCG
CGTGCCCCAGCCGCGCAACGAAGAGGAAGAAATGTCGGGCGATATCTGGTACACCGTGCGGCCGCACGACATCTTTCCGG
AGACGTTCCGTACTTTCCTGCTCGGGAACCCGCGCGTGCGCGAGGCGTTCCTGCGCCATCACGAAGATTTATTCGACCCT
GCCATGTGGCAGAGCCATAAAGACCGGCTGCTGGCCGGACATGTTCATGACTTCTTTGCCTATCCCATTTCTGAACGCTT
CATCAACCGCTACGGCACGGCTGCGCACGGCACGCCCGTCGCCAGTTTCGCAAGGAGAGTCGCATGA

Upstream 100 bases:

>100_bases
GCGGCTATTCGGAAGGTATTCGCCACTGAGCACCCCAAATCGAGTCCCGAAACCCATTTACAATCCCACTAGCCCCCGCC
AGAACGCGTCCACTCGTCTC

Downstream 100 bases:

>100_bases
GTGACGCTATCGCCCAGCTGTTCCGCAACAACCGCGAATGGGTCGACCGCGTCAACGCGGAAGATCCGACCTTCTTCATG
CGCCTGGCCAACCAGCAGGC

Product: bifunctional isocitrate dehydrogenase kinase/phosphatase protein

Products: NA

Alternate protein names: IDH kinase/phosphatase; IDHK/P

Number of amino acids: Translated: 608; Mature: 607

Protein sequence:

>608_residues
MSHFPKLLSSQIAFDVARTMLDGFDKHYRLFREVSRQAKVKFEAGDWHSLQQMQRDRIAFYNERVRETSVILEDEYDAEN
IEDEIWQQIKLHYIGLLTNHHQPELAETFFNSVCTRLLHRSYFNNDFIFVRPAISTEYIENEESPTRPTFRAYYPGSRQG
MAECFERIVHNFQLETPFEDLRRDVGHVVRGVEEHFGDFRIAPNFQIHVLSSLFFRNKTAFIIGRIVNADRTYPLAIPIV
HGPSGKLTLDAVLLKKVQVLILFSFTHSYFMVDMEIPSAYVTFLRDIMPRKPRAEIYTSLGLQKQGKNLFYRDFLHHLQH
SSDKFISAPGIKGLVMLVFTLPSYPYVFKVIKDFYPAPKETTRELIKSKYQLVKQHDRVGRMADTLEYSNVAFPLSRFDE
DLVRELEHHAPSMIEYQRGKDGGEEIVVRHVYIERRMTPLNIWLQEGTDEQLEHGIIEYGNAIKELIAANIFPGDMLYKN
FGVTRHGRVVFYDYDEIEYFTDCNVRRVPQPRNEEEEMSGDIWYTVRPHDIFPETFRTFLLGNPRVREAFLRHHEDLFDP
AMWQSHKDRLLAGHVHDFFAYPISERFINRYGTAAHGTPVASFARRVA

Sequences:

>Translated_608_residues
MSHFPKLLSSQIAFDVARTMLDGFDKHYRLFREVSRQAKVKFEAGDWHSLQQMQRDRIAFYNERVRETSVILEDEYDAEN
IEDEIWQQIKLHYIGLLTNHHQPELAETFFNSVCTRLLHRSYFNNDFIFVRPAISTEYIENEESPTRPTFRAYYPGSRQG
MAECFERIVHNFQLETPFEDLRRDVGHVVRGVEEHFGDFRIAPNFQIHVLSSLFFRNKTAFIIGRIVNADRTYPLAIPIV
HGPSGKLTLDAVLLKKVQVLILFSFTHSYFMVDMEIPSAYVTFLRDIMPRKPRAEIYTSLGLQKQGKNLFYRDFLHHLQH
SSDKFISAPGIKGLVMLVFTLPSYPYVFKVIKDFYPAPKETTRELIKSKYQLVKQHDRVGRMADTLEYSNVAFPLSRFDE
DLVRELEHHAPSMIEYQRGKDGGEEIVVRHVYIERRMTPLNIWLQEGTDEQLEHGIIEYGNAIKELIAANIFPGDMLYKN
FGVTRHGRVVFYDYDEIEYFTDCNVRRVPQPRNEEEEMSGDIWYTVRPHDIFPETFRTFLLGNPRVREAFLRHHEDLFDP
AMWQSHKDRLLAGHVHDFFAYPISERFINRYGTAAHGTPVASFARRVA
>Mature_607_residues
SHFPKLLSSQIAFDVARTMLDGFDKHYRLFREVSRQAKVKFEAGDWHSLQQMQRDRIAFYNERVRETSVILEDEYDAENI
EDEIWQQIKLHYIGLLTNHHQPELAETFFNSVCTRLLHRSYFNNDFIFVRPAISTEYIENEESPTRPTFRAYYPGSRQGM
AECFERIVHNFQLETPFEDLRRDVGHVVRGVEEHFGDFRIAPNFQIHVLSSLFFRNKTAFIIGRIVNADRTYPLAIPIVH
GPSGKLTLDAVLLKKVQVLILFSFTHSYFMVDMEIPSAYVTFLRDIMPRKPRAEIYTSLGLQKQGKNLFYRDFLHHLQHS
SDKFISAPGIKGLVMLVFTLPSYPYVFKVIKDFYPAPKETTRELIKSKYQLVKQHDRVGRMADTLEYSNVAFPLSRFDED
LVRELEHHAPSMIEYQRGKDGGEEIVVRHVYIERRMTPLNIWLQEGTDEQLEHGIIEYGNAIKELIAANIFPGDMLYKNF
GVTRHGRVVFYDYDEIEYFTDCNVRRVPQPRNEEEEMSGDIWYTVRPHDIFPETFRTFLLGNPRVREAFLRHHEDLFDPA
MWQSHKDRLLAGHVHDFFAYPISERFINRYGTAAHGTPVASFARRVA

Specific function: Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source

COG id: COG4579

COG function: function code T; Isocitrate dehydrogenase kinase/phosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AceK family

Homologues:

Organism=Escherichia coli, GI1790446, Length=583, Percent_Identity=41.1663807890223, Blast_Score=459, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ACEK_CUPPJ (Q477B5)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_294362.1
- GeneID:   3610949
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A0136
- NMPDR:   fig|264198.3.peg.648
- HOGENOM:   HBG298593
- OMA:   RMTPLNI
- ProtClustDB:   PRK02946
- BioCyc:   REUT264198:REUT_A0136-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00747
- InterPro:   IPR010452
- PIRSF:   PIRSF000719
- ProDom:   PD043552

Pfam domain/function: PF06315 AceK

EC number: =2.7.11.5

Molecular weight: Translated: 71456; Mature: 71325

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: ACT_SITE 384-384 BINDING 349-349

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHFPKLLSSQIAFDVARTMLDGFDKHYRLFREVSRQAKVKFEAGDWHSLQQMQRDRIAF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHH
YNERVRETSVILEDEYDAENIEDEIWQQIKLHYIGLLTNHHQPELAETFFNSVCTRLLHR
HHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
SYFNNDFIFVRPAISTEYIENEESPTRPTFRAYYPGSRQGMAECFERIVHNFQLETPFED
HHCCCCEEEEECCCCHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCHHH
LRRDVGHVVRGVEEHFGDFRIAPNFQIHVLSSLFFRNKTAFIIGRIVNADRTYPLAIPIV
HHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEEEEE
HGPSGKLTLDAVLLKKVQVLILFSFTHSYFMVDMEIPSAYVTFLRDIMPRKPRAEIYTSL
ECCCCCEEHHHHHHHHHHHHHHEEECCCEEEEEEECCHHHHHHHHHHCCCCCHHHHHHHH
GLQKQGKNLFYRDFLHHLQHSSDKFISAPGIKGLVMLVFTLPSYPYVFKVIKDFYPAPKE
CCHHCCCCHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHH
TTRELIKSKYQLVKQHDRVGRMADTLEYSNVAFPLSRFDEDLVRELEHHAPSMIEYQRGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHCCCCHHHHHCCC
DGGEEIVVRHVYIERRMTPLNIWLQEGTDEQLEHGIIEYGNAIKELIAANIFPGDMLYKN
CCCHHHEEHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
FGVTRHGRVVFYDYDEIEYFTDCNVRRVPQPRNEEEEMSGDIWYTVRPHDIFPETFRTFL
CCCCCCCEEEEEECCCCCEECCCCCEECCCCCCCHHHCCCCEEEEECCCCCCHHHHHHHH
LGNPRVREAFLRHHEDLFDPAMWQSHKDRLLAGHVHDFFAYPISERFINRYGTAAHGTPV
CCCHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCHH
ASFARRVA
HHHHHHCC
>Mature Secondary Structure 
SHFPKLLSSQIAFDVARTMLDGFDKHYRLFREVSRQAKVKFEAGDWHSLQQMQRDRIAF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHH
YNERVRETSVILEDEYDAENIEDEIWQQIKLHYIGLLTNHHQPELAETFFNSVCTRLLHR
HHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
SYFNNDFIFVRPAISTEYIENEESPTRPTFRAYYPGSRQGMAECFERIVHNFQLETPFED
HHCCCCEEEEECCCCHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCHHH
LRRDVGHVVRGVEEHFGDFRIAPNFQIHVLSSLFFRNKTAFIIGRIVNADRTYPLAIPIV
HHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEEEEE
HGPSGKLTLDAVLLKKVQVLILFSFTHSYFMVDMEIPSAYVTFLRDIMPRKPRAEIYTSL
ECCCCCEEHHHHHHHHHHHHHHEEECCCEEEEEEECCHHHHHHHHHHCCCCCHHHHHHHH
GLQKQGKNLFYRDFLHHLQHSSDKFISAPGIKGLVMLVFTLPSYPYVFKVIKDFYPAPKE
CCHHCCCCHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHH
TTRELIKSKYQLVKQHDRVGRMADTLEYSNVAFPLSRFDEDLVRELEHHAPSMIEYQRGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHCCCCHHHHHCCC
DGGEEIVVRHVYIERRMTPLNIWLQEGTDEQLEHGIIEYGNAIKELIAANIFPGDMLYKN
CCCHHHEEHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
FGVTRHGRVVFYDYDEIEYFTDCNVRRVPQPRNEEEEMSGDIWYTVRPHDIFPETFRTFL
CCCCCCCEEEEEECCCCCEECCCCCEECCCCCCCHHHCCCCEEEEECCCCCCHHHHHHHH
LGNPRVREAFLRHHEDLFDPAMWQSHKDRLLAGHVHDFFAYPISERFINRYGTAAHGTPV
CCCHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCHH
ASFARRVA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA