Click here to switch to the map view.

The map label for this gene is metH [H]

Identifier: 73539824

GI number: 73539824

Start: 144141

End: 145196

Strand: Direct

Name: metH [H]

Synonym: Reut_A0118

Alternate gene names: 73539824

Gene position: 144141-145196 (Clockwise)

Preceding gene: 73539822

Following gene: 73539825

Centisome position: 3.79

GC content: 64.49

Gene sequence:

>1056_bases
ATGAGTGCCCCTGAACAACCCTCCGTGCGTCCCTATACACGGGCTGCCAATCTGCCCCGGCTGCTGCGCGAGCGCATCCT
GATCCTGGACGGCGCCATGGGCACCATGATCCAGCGCTACAAACTGACCGAAGCCGACTATCGCGGCGAGCGCTTCGCGG
AACACAAGGTCGACGTGAAGGGCAACAACGAATTGCTGCTGCTCACGCGTCCGCAGGTCATCAGCGAAATTCACGAGCAG
TACCTCGCCGCCGGCGCGGACATCATCGAGACCAATACCTTCGGCGCCACAAGTGTGGCGCAGGAGGACTACAAGATGGC
CGAGCTCGCGTACGAGATGAATGTCGAAGCCGCGAAGCTCGCGCGCGCGGCCTGCGACAAGTACAGCACGCCTGACAAGC
CGCGCTTCGTGGCCGGCGCGTTCGGCCCCACGCCGAAGACAGCCAGCATCTCGCCCGACGTGAATGATCCGGGCGCGCGC
AATATCACGTTCGAGGAACTGCGCGAGTCCTACTACGAGCAGGGCAAGGGCTTGCTCGATGGCGGCGCCGATGTGTTCCT
CGTGGAAACGATCTTCGACACGCTCAACGCCAAGGCGTGCCTGTTCGCGATCGATCAGCTCTTCGAAGACACCGGCGAGC
GCCTGCCCGTGATGATCTCCGGCACCGTGACCGATGCGTCGGGCCGCATTCTCTCGGGCCAGACCGTCGAAGCGTTCTGG
AACAGCCTGCGCCATGTGAAGCCCGTCACGTTCGGCCTGAACTGCGCGCTGGGCGCGACGCTGATGCGTCCATACATCGC
CGAACTGGCGAAGGTCTGCGACGTGGCGATTTCTTGCTACCCGAACGCGGGCCTCCCGAATCCGATGAGCGAAACCGGCT
TCGACGAGACGCCGGAAGTCACTTCGTCGCTGGTCGACGAATTCGCCGCCTCGGGGCTGGTGAACCTGGTGGGCGGCTGC
TGCGGCACCACGCCTGAACACATTGCCGCCATCGCCGAGCGCATGGCGAAGCGGAACCCGCGCACGTGGCCGGGCCAGTA
CAGCGAAGCCGCCTGA

Upstream 100 bases:

>100_bases
CCAGACCGTATGCAGGCAACCCGCCACACCCGCAAGGCCTTGATGCACCTGATAAAATCGCCGTCTTGACTATCCGTCCC
GCCTTCTAACAGTCCCCGCC

Downstream 100 bases:

>100_bases
GCCAGCGAGACAACAAGACATGAGCACGAACCAACTGCCCCCGCGCCCGATGCGCCTGTCCGGCCTGGAGCCGTTCACGA
TCGACGACGACACGCTGTTC

Product: methionine synthase (B12-dependent)

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MSAPEQPSVRPYTRAANLPRLLRERILILDGAMGTMIQRYKLTEADYRGERFAEHKVDVKGNNELLLLTRPQVISEIHEQ
YLAAGADIIETNTFGATSVAQEDYKMAELAYEMNVEAAKLARAACDKYSTPDKPRFVAGAFGPTPKTASISPDVNDPGAR
NITFEELRESYYEQGKGLLDGGADVFLVETIFDTLNAKACLFAIDQLFEDTGERLPVMISGTVTDASGRILSGQTVEAFW
NSLRHVKPVTFGLNCALGATLMRPYIAELAKVCDVAISCYPNAGLPNPMSETGFDETPEVTSSLVDEFAASGLVNLVGGC
CGTTPEHIAAIAERMAKRNPRTWPGQYSEAA

Sequences:

>Translated_351_residues
MSAPEQPSVRPYTRAANLPRLLRERILILDGAMGTMIQRYKLTEADYRGERFAEHKVDVKGNNELLLLTRPQVISEIHEQ
YLAAGADIIETNTFGATSVAQEDYKMAELAYEMNVEAAKLARAACDKYSTPDKPRFVAGAFGPTPKTASISPDVNDPGAR
NITFEELRESYYEQGKGLLDGGADVFLVETIFDTLNAKACLFAIDQLFEDTGERLPVMISGTVTDASGRILSGQTVEAFW
NSLRHVKPVTFGLNCALGATLMRPYIAELAKVCDVAISCYPNAGLPNPMSETGFDETPEVTSSLVDEFAASGLVNLVGGC
CGTTPEHIAAIAERMAKRNPRTWPGQYSEAA
>Mature_350_residues
SAPEQPSVRPYTRAANLPRLLRERILILDGAMGTMIQRYKLTEADYRGERFAEHKVDVKGNNELLLLTRPQVISEIHEQY
LAAGADIIETNTFGATSVAQEDYKMAELAYEMNVEAAKLARAACDKYSTPDKPRFVAGAFGPTPKTASISPDVNDPGARN
ITFEELRESYYEQGKGLLDGGADVFLVETIFDTLNAKACLFAIDQLFEDTGERLPVMISGTVTDASGRILSGQTVEAFWN
SLRHVKPVTFGLNCALGATLMRPYIAELAKVCDVAISCYPNAGLPNPMSETGFDETPEVTSSLVDEFAASGLVNLVGGCC
GTTPEHIAAIAERMAKRNPRTWPGQYSEAA

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=340, Percent_Identity=53.2352941176471, Blast_Score=363, Evalue=1e-100,
Organism=Escherichia coli, GI1790450, Length=334, Percent_Identity=55.9880239520958, Blast_Score=380, Evalue=1e-107,
Organism=Escherichia coli, GI1786456, Length=323, Percent_Identity=27.2445820433437, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17535405, Length=329, Percent_Identity=52.5835866261398, Blast_Score=352, Evalue=2e-97,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 38187; Mature: 38055

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPEQPSVRPYTRAANLPRLLRERILILDGAMGTMIQRYKLTEADYRGERFAEHKVDVK
CCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHCCCEEC
GNNELLLLTRPQVISEIHEQYLAAGADIIETNTFGATSVAQEDYKMAELAYEMNVEAAKL
CCCCEEEEECHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHH
ARAACDKYSTPDKPRFVAGAFGPTPKTASISPDVNDPGARNITFEELRESYYEQGKGLLD
HHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEC
GGADVFLVETIFDTLNAKACLFAIDQLFEDTGERLPVMISGTVTDASGRILSGQTVEAFW
CCCCEEHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEECCCCHHHHH
NSLRHVKPVTFGLNCALGATLMRPYIAELAKVCDVAISCYPNAGLPNPMSETGFDETPEV
HHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
TSSLVDEFAASGLVNLVGGCCGTTPEHIAAIAERMAKRNPRTWPGQYSEAA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SAPEQPSVRPYTRAANLPRLLRERILILDGAMGTMIQRYKLTEADYRGERFAEHKVDVK
CCCCCCCCCCHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHCCCEEC
GNNELLLLTRPQVISEIHEQYLAAGADIIETNTFGATSVAQEDYKMAELAYEMNVEAAKL
CCCCEEEEECHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHH
ARAACDKYSTPDKPRFVAGAFGPTPKTASISPDVNDPGARNITFEELRESYYEQGKGLLD
HHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEC
GGADVFLVETIFDTLNAKACLFAIDQLFEDTGERLPVMISGTVTDASGRILSGQTVEAFW
CCCCEEHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEECCCCHHHHH
NSLRHVKPVTFGLNCALGATLMRPYIAELAKVCDVAISCYPNAGLPNPMSETGFDETPEV
HHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
TSSLVDEFAASGLVNLVGGCCGTTPEHIAAIAERMAKRNPRTWPGQYSEAA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]