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The map label for this gene is hipO [H]

Identifier: 73539757

GI number: 73539757

Start: 65610

End: 66833

Strand: Direct

Name: hipO [H]

Synonym: Reut_A0051

Alternate gene names: 73539757

Gene position: 65610-66833 (Clockwise)

Preceding gene: 73539756

Following gene: 73539759

Centisome position: 1.72

GC content: 63.81

Gene sequence:

>1224_bases
ATGTCCGAGCACGCCCCCGAGATCGAGTCTTACCTCGGTACCTCTCTGAAGCGCCGCTTCTGCGCGCTGGCCGATACCCT
CGACAGCCGCGACGAACTCGAAGCCATCCGGCGCAATATTCACCAGAATCCAGAACTCGCGTTCGACGAAGTCCGCACGT
CCGGGCTCGTTGCCAGCCTGCTCGAAGGCTGGGGGTACACCGTCACGCGCGGCGTCGGCGGGACCGGGCTGGTCGGCACG
TTGAAGGCGGGGACAGGGGGGCGCAGCGTTGGCATTCGCGCGGACATGGATGCATTGCCCATTCATGAGCGGACCGGCCT
GCCATACGCGAGCGTCAACGAAGGGCGCATGCACGCGTGCGGTCACGACGGCCACACCACGATCCTGCTCGGCGCCGCAA
AGCAGCTGGCGCGCACGCTTAACTTCAACGGTACCGTACACCTGATCTTCCAGCCGGCCGAGGAAATCGGCGCTGGCGGC
GGTGCTGAACGCATGCTGGCCGATGGACTGTTCTATCGCTTTCCGTGCGATGCCATCTTCGGCCTGCATAACCATCCGGG
CGTGGAGCAGGGGAATTTCCTGTTCCGCTCGGGACCGTTCATGGCGGCCTGCGACACGGTGACCATTACCATTCGCGGCA
AGGGGGGGCATGCGGCGCGCCCGCACCAGTCGGTCGATCCGATTCTCGTTGCCGGCAGCCTGGTGATGGCGCTGCAGTCG
GTGGTTTCGCGCTATGTGGATCCGAATGAAACCGCGGTGGTCACCATCGGCACGCTGCACGCGGGCCATGCACCTAATGT
GATCCCGGACAGCGCGCGCATGGAGATCAGCGTGCGCTCGTTCAGCCCGGATGTGCGGGCTTCGCTGGAAACGCGCATCC
GGCAGATGGCGATCGCGCACGCGGAGGGATATGGCGCCGTGGCGGAAGTGAATTATGTGCATGGCTATCCGGTGCTGATC
AACAGCGAACGGGAAACCGAATTCGCGCGTCAGATCGCGGAGGAACTGGTCGGTGCCGACAAGGTAGTTTCGCAAGCGGC
CCGGATCACGGGCAGCGAGGATTTCGCGTATTACCTGCAGCAAAGGCCGGGCTGTTTCGTGCGGCTCGGTAATGGTGCCA
ATCAGCCCCTGCTGCACAACCCGGCGTACGACTTCAATGACGAGAACCTGACGGTAGGTGCGGCCTATTGGACCAGACTG
GTGGAGCGCTACCTGGCAAGGTGA

Upstream 100 bases:

>100_bases
ATTCGGCTATTGGCCTTTCCGTTTGCGATTTCTGCGTGAAATACTGTTTATAAGGACGGCGCACTGACCGTCCCGCAGTC
TCTGCCAGAAGAGCAATGCC

Downstream 100 bases:

>100_bases
CCTCGCTGTTTGCGATGCTGCATGCGGCATCATGGCTTTAGCGCCCCAAGATAGCCTCCTTCGGAGGCTACTTCTTCTTT
TTCTCGCCAAAGAAGAAGCA

Product: peptidase M20D, amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MSEHAPEIESYLGTSLKRRFCALADTLDSRDELEAIRRNIHQNPELAFDEVRTSGLVASLLEGWGYTVTRGVGGTGLVGT
LKAGTGGRSVGIRADMDALPIHERTGLPYASVNEGRMHACGHDGHTTILLGAAKQLARTLNFNGTVHLIFQPAEEIGAGG
GAERMLADGLFYRFPCDAIFGLHNHPGVEQGNFLFRSGPFMAACDTVTITIRGKGGHAARPHQSVDPILVAGSLVMALQS
VVSRYVDPNETAVVTIGTLHAGHAPNVIPDSARMEISVRSFSPDVRASLETRIRQMAIAHAEGYGAVAEVNYVHGYPVLI
NSERETEFARQIAEELVGADKVVSQAARITGSEDFAYYLQQRPGCFVRLGNGANQPLLHNPAYDFNDENLTVGAAYWTRL
VERYLAR

Sequences:

>Translated_407_residues
MSEHAPEIESYLGTSLKRRFCALADTLDSRDELEAIRRNIHQNPELAFDEVRTSGLVASLLEGWGYTVTRGVGGTGLVGT
LKAGTGGRSVGIRADMDALPIHERTGLPYASVNEGRMHACGHDGHTTILLGAAKQLARTLNFNGTVHLIFQPAEEIGAGG
GAERMLADGLFYRFPCDAIFGLHNHPGVEQGNFLFRSGPFMAACDTVTITIRGKGGHAARPHQSVDPILVAGSLVMALQS
VVSRYVDPNETAVVTIGTLHAGHAPNVIPDSARMEISVRSFSPDVRASLETRIRQMAIAHAEGYGAVAEVNYVHGYPVLI
NSERETEFARQIAEELVGADKVVSQAARITGSEDFAYYLQQRPGCFVRLGNGANQPLLHNPAYDFNDENLTVGAAYWTRL
VERYLAR
>Mature_406_residues
SEHAPEIESYLGTSLKRRFCALADTLDSRDELEAIRRNIHQNPELAFDEVRTSGLVASLLEGWGYTVTRGVGGTGLVGTL
KAGTGGRSVGIRADMDALPIHERTGLPYASVNEGRMHACGHDGHTTILLGAAKQLARTLNFNGTVHLIFQPAEEIGAGGG
AERMLADGLFYRFPCDAIFGLHNHPGVEQGNFLFRSGPFMAACDTVTITIRGKGGHAARPHQSVDPILVAGSLVMALQSV
VSRYVDPNETAVVTIGTLHAGHAPNVIPDSARMEISVRSFSPDVRASLETRIRQMAIAHAEGYGAVAEVNYVHGYPVLIN
SERETEFARQIAEELVGADKVVSQAARITGSEDFAYYLQQRPGCFVRLGNGANQPLLHNPAYDFNDENLTVGAAYWTRLV
ERYLAR

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=414, Percent_Identity=27.2946859903382, Blast_Score=99, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 44019; Mature: 43888

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEHAPEIESYLGTSLKRRFCALADTLDSRDELEAIRRNIHQNPELAFDEVRTSGLVASL
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LEGWGYTVTRGVGGTGLVGTLKAGTGGRSVGIRADMDALPIHERTGLPYASVNEGRMHAC
HHCCCEEEECCCCCCCCEEEEECCCCCCEECEEECCCCCCCHHHCCCCCCCCCCCCEEEC
GHDGHTTILLGAAKQLARTLNFNGTVHLIFQPAEEIGAGGGAERMLADGLFYRFPCDAIF
CCCCCEEEEEHHHHHHHHHHCCCCEEEEEEECHHHHCCCCCHHHHHHCCCEEECCHHHHH
GLHNHPGVEQGNFLFRSGPFMAACDTVTITIRGKGGHAARPHQSVDPILVAGSLVMALQS
CCCCCCCCCCCCEEEECCCCEEEECEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
VVSRYVDPNETAVVTIGTLHAGHAPNVIPDSARMEISVRSFSPDVRASLETRIRQMAIAH
HHHHCCCCCCCEEEEEEEEECCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
AEGYGAVAEVNYVHGYPVLINSERETEFARQIAEELVGADKVVSQAARITGSEDFAYYLQ
CCCCCCEEEEEEECCCEEEEECCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHH
QRPGCFVRLGNGANQPLLHNPAYDFNDENLTVGAAYWTRLVERYLAR
CCCCEEEEECCCCCCCCEECCCCCCCCCCEEEHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEHAPEIESYLGTSLKRRFCALADTLDSRDELEAIRRNIHQNPELAFDEVRTSGLVASL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LEGWGYTVTRGVGGTGLVGTLKAGTGGRSVGIRADMDALPIHERTGLPYASVNEGRMHAC
HHCCCEEEECCCCCCCCEEEEECCCCCCEECEEECCCCCCCHHHCCCCCCCCCCCCEEEC
GHDGHTTILLGAAKQLARTLNFNGTVHLIFQPAEEIGAGGGAERMLADGLFYRFPCDAIF
CCCCCEEEEEHHHHHHHHHHCCCCEEEEEEECHHHHCCCCCHHHHHHCCCEEECCHHHHH
GLHNHPGVEQGNFLFRSGPFMAACDTVTITIRGKGGHAARPHQSVDPILVAGSLVMALQS
CCCCCCCCCCCCEEEECCCCEEEECEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
VVSRYVDPNETAVVTIGTLHAGHAPNVIPDSARMEISVRSFSPDVRASLETRIRQMAIAH
HHHHCCCCCCCEEEEEEEEECCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
AEGYGAVAEVNYVHGYPVLINSERETEFARQIAEELVGADKVVSQAARITGSEDFAYYLQ
CCCCCCEEEEEEECCCEEEEECCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHH
QRPGCFVRLGNGANQPLLHNPAYDFNDENLTVGAAYWTRLVERYLAR
CCCCEEEEECCCCCCCCEECCCCCCCCCCEEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]