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The map label for this gene is pucL [H]

Identifier: 73539741

GI number: 73539741

Start: 47309

End: 47836

Strand: Direct

Name: pucL [H]

Synonym: Reut_A0035

Alternate gene names: 73539741

Gene position: 47309-47836 (Clockwise)

Preceding gene: 73539740

Following gene: 73539743

Centisome position: 1.24

GC content: 64.02

Gene sequence:

>528_bases
ATGTCACAGTCAATCGATCTCGCGCCGGTCAACGCGCTGGACCAGGCGGGCTTTGCGCGCGTGTTCGGCAGCGTGTTCGA
ACACTTTCCGCAGGCCGCGGCAGGGGCCTGGTCGCAGCGCCCGTTTGCATCGGTGGCGGCGCTGCATGAGGCGATGATGG
ACGTGGTACGCAATCTCGACGACAAGGGCCAGCGCGATTTCCTGAACCTGCACCCGTTGCTGTCCGGCGCCAACATCCGC
GCAGGCACGATGACGGCGGATTCGAACGCGGAGCAGAAGAGCGCTGGATTGGACGCGATGACGACGCAGCAGGAAGACAC
GCTCGATCGCATGAACACCGCGTACCTCGCGCGCCATGGTTTCCCATTCATCATCTGCGTGCGGCATTACACGCGTGAAG
GGATTTTTGCGCAGCTGGAGCGCCGCGTCGATCGCAGCACACCGGTGGAGCTTGAAGAGGCGCTGGCGCAGATCGCGGCG
ATTACGCGCGGGCGCCTCAACGCCCGGCTCAATACACTGGCGTCATGA

Upstream 100 bases:

>100_bases
CTGCCGTGGGTGCTTCCTATACTCGGCCCGTATTCGACGTTCACAACGTCCACAACGTCCACGCCTTGCCAACGCGGCGG
GCCTTACGGGAGAGCAGTCC

Downstream 100 bases:

>100_bases
TTCCTGGCAGCGTGCCGGCGCAATATTGCGCGGCACGCTGCCAGGGCTCGGGAACTCAACCGAGCCTGAACTGCCCCACC
GCCCCCGCCAGGCTTGCCGC

Product: putative uricase

Products: NA

Alternate protein names: 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase; OHCU decarboxylase; Uricase; Urate oxidase [H]

Number of amino acids: Translated: 175; Mature: 174

Protein sequence:

>175_residues
MSQSIDLAPVNALDQAGFARVFGSVFEHFPQAAAGAWSQRPFASVAALHEAMMDVVRNLDDKGQRDFLNLHPLLSGANIR
AGTMTADSNAEQKSAGLDAMTTQQEDTLDRMNTAYLARHGFPFIICVRHYTREGIFAQLERRVDRSTPVELEEALAQIAA
ITRGRLNARLNTLAS

Sequences:

>Translated_175_residues
MSQSIDLAPVNALDQAGFARVFGSVFEHFPQAAAGAWSQRPFASVAALHEAMMDVVRNLDDKGQRDFLNLHPLLSGANIR
AGTMTADSNAEQKSAGLDAMTTQQEDTLDRMNTAYLARHGFPFIICVRHYTREGIFAQLERRVDRSTPVELEEALAQIAA
ITRGRLNARLNTLAS
>Mature_174_residues
SQSIDLAPVNALDQAGFARVFGSVFEHFPQAAAGAWSQRPFASVAALHEAMMDVVRNLDDKGQRDFLNLHPLLSGANIRA
GTMTADSNAEQKSAGLDAMTTQQEDTLDRMNTAYLARHGFPFIICVRHYTREGIFAQLERRVDRSTPVELEEALAQIAAI
TRGRLNARLNTLAS

Specific function: Catalyzes two steps in the degradation of uric acid, i.e. the oxidation of uric acid to 5-hydroxyisourate (HIU) and the stereoselective decarboxylation of 2-oxo-4-hydroxy-4-carboxy-5- ureidoimidazoline (OHCU) to (S)-allantoin [H]

COG id: COG3195

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the uricase family [H]

Homologues:

Organism=Homo sapiens, GI157743280, Length=162, Percent_Identity=35.8024691358025, Blast_Score=107, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018020
- InterPro:   IPR017580
- InterPro:   IPR002042
- InterPro:   IPR019842 [H]

Pfam domain/function: PF09349 OHCU_decarbox; PF01014 Uricase [H]

EC number: =1.7.3.3 [H]

Molecular weight: Translated: 19215; Mature: 19084

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSIDLAPVNALDQAGFARVFGSVFEHFPQAAAGAWSQRPFASVAALHEAMMDVVRNLD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
DKGQRDFLNLHPLLSGANIRAGTMTADSNAEQKSAGLDAMTTQQEDTLDRMNTAYLARHG
CCCCHHHHHHHHHHCCCCCCCCCEECCCCCHHHHCCCCHHHCCHHHHHHHHHHHHHHHCC
FPFIICVRHYTREGIFAQLERRVDRSTPVELEEALAQIAAITRGRLNARLNTLAS
CCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQSIDLAPVNALDQAGFARVFGSVFEHFPQAAAGAWSQRPFASVAALHEAMMDVVRNLD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
DKGQRDFLNLHPLLSGANIRAGTMTADSNAEQKSAGLDAMTTQQEDTLDRMNTAYLARHG
CCCCHHHHHHHHHHCCCCCCCCCEECCCCCHHHHCCCCHHHCCHHHHHHHHHHHHHHHCC
FPFIICVRHYTREGIFAQLERRVDRSTPVELEEALAQIAAITRGRLNARLNTLAS
CCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]