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The map label for this gene is dnaN [H]

Identifier: 73539708

GI number: 73539708

Start: 2393

End: 3508

Strand: Direct

Name: dnaN [H]

Synonym: Reut_A0002

Alternate gene names: 73539708

Gene position: 2393-3508 (Clockwise)

Preceding gene: 161611268

Following gene: 73539709

Centisome position: 0.06

GC content: 61.56

Gene sequence:

>1116_bases
ATGCAATTGGTCAAAACCTCGCGAGACAATCTGCTGCGTCCGCTGCAAATCGTGAGCGGCATCGTGGAGCGCCGCCACAC
CCTCCCGATCCTGGCCAACCTGCTGATTCGCAAGTCCGGCTCGAACGTATCCTTCCTCTCGACCGACATCGAGATCCAGA
TCACCACGCACGCCGAATGCGGCGTGGGCAATGACAGCGTGGCCACCACCGTGGCCGCACGCAAGCTGCTCGACATCCTG
CGCGCCATGCCCGACGGCGACGTCGCCCTGTCGCTCAACGACAAGCGCATGACCGTGCAGTCCGGCAAGAGCCGCTTCGC
GCTGCAGACGCTGGCCGCCGAAGAATTCCCGACTGTTGCCGAAGCGAGCGAATTCAACGCGAGCGTTTCGCTGCCGCAGA
AGACCTTCAAGCACCTGCTGGCGATGGTCCACTTCGCCATGGCGCAGCAAGACATCCGTTACTACCTCAACGGCATGCTG
CTCGTCGTCGACGGCAAGAAGGTCATGGCAGTCGCCACCGACGGCCACCGCCTGGCCTACTGCGGCGTGGAGCTGGAAAA
CGAAGCCACCGGTGTGGGCTCGCGCCAGGAAGTCATCATCCCGCGCAAGACCATCCTGGAACTGCAACGCCTGCTCGAAG
ACAACGACGATCCCGTGCAGGTGCAGCTCGCCGCCAACCAGGTCAAGTTCAGCTTCGCCAATATCGAGCTGATCTCCAAG
CTGGTCGAAGGCAAGTTCCCCGACTTCCAGCGCGTCATTCCCAAGGGCTACAAGAACGCCTTTGCGATCGACCGCGTGCG
CCTGCAGCAGGCGCTGCAACGTACCGCCATCCTGACGACTGACAAGTTCAAGGGCGTGCGCTGCATCCTGGACACCCACA
TGCTGAAGATCAGCTCCACCAACGCCGATCAGGAAGAGGCGCAGGAAGAGCTGGAAATCGATTACTCGGGCGACGCGCTC
GACATCGGCTTCAACGTGACCTACCTGCTCGACGTCCTCGCCAACCTGAAGAGCGAGCAAGTGCAGGTCAGCCTCGGCGA
CTCGAATTCGAGTGCGCTGATCACCGTGCCGGAAGACGACAACTTCAAGTACGTCGTCATGCCGATGCGCATCTGA

Upstream 100 bases:

>100_bases
TATCCTTGGTACAATGGCACATTAACTCCTTGATTTCTAAGTGAATTGGAGTTGCCTGGAGTTTGCGGTCGCCGACGACA
AACGACGAAGGATAAATTCA

Downstream 100 bases:

>100_bases
TGATCCGGTAACAGGACGACAGATACAGAGATACCGGAGCAGCAAACGGCACGCTACGACACCCGCACTATCACGGGACG
TCTCTTTGCAGCAGGGGCAG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MQLVKTSRDNLLRPLQIVSGIVERRHTLPILANLLIRKSGSNVSFLSTDIEIQITTHAECGVGNDSVATTVAARKLLDIL
RAMPDGDVALSLNDKRMTVQSGKSRFALQTLAAEEFPTVAEASEFNASVSLPQKTFKHLLAMVHFAMAQQDIRYYLNGML
LVVDGKKVMAVATDGHRLAYCGVELENEATGVGSRQEVIIPRKTILELQRLLEDNDDPVQVQLAANQVKFSFANIELISK
LVEGKFPDFQRVIPKGYKNAFAIDRVRLQQALQRTAILTTDKFKGVRCILDTHMLKISSTNADQEEAQEELEIDYSGDAL
DIGFNVTYLLDVLANLKSEQVQVSLGDSNSSALITVPEDDNFKYVVMPMRI

Sequences:

>Translated_371_residues
MQLVKTSRDNLLRPLQIVSGIVERRHTLPILANLLIRKSGSNVSFLSTDIEIQITTHAECGVGNDSVATTVAARKLLDIL
RAMPDGDVALSLNDKRMTVQSGKSRFALQTLAAEEFPTVAEASEFNASVSLPQKTFKHLLAMVHFAMAQQDIRYYLNGML
LVVDGKKVMAVATDGHRLAYCGVELENEATGVGSRQEVIIPRKTILELQRLLEDNDDPVQVQLAANQVKFSFANIELISK
LVEGKFPDFQRVIPKGYKNAFAIDRVRLQQALQRTAILTTDKFKGVRCILDTHMLKISSTNADQEEAQEELEIDYSGDAL
DIGFNVTYLLDVLANLKSEQVQVSLGDSNSSALITVPEDDNFKYVVMPMRI
>Mature_371_residues
MQLVKTSRDNLLRPLQIVSGIVERRHTLPILANLLIRKSGSNVSFLSTDIEIQITTHAECGVGNDSVATTVAARKLLDIL
RAMPDGDVALSLNDKRMTVQSGKSRFALQTLAAEEFPTVAEASEFNASVSLPQKTFKHLLAMVHFAMAQQDIRYYLNGML
LVVDGKKVMAVATDGHRLAYCGVELENEATGVGSRQEVIIPRKTILELQRLLEDNDDPVQVQLAANQVKFSFANIELISK
LVEGKFPDFQRVIPKGYKNAFAIDRVRLQQALQRTAILTTDKFKGVRCILDTHMLKISSTNADQEEAQEELEIDYSGDAL
DIGFNVTYLLDVLANLKSEQVQVSLGDSNSSALITVPEDDNFKYVVMPMRI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=365, Percent_Identity=43.2876712328767, Blast_Score=307, Evalue=6e-85,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41137; Mature: 41137

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLVKTSRDNLLRPLQIVSGIVERRHTLPILANLLIRKSGSNVSFLSTDIEIQITTHAEC
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEEEEEEEEEECCCC
GVGNDSVATTVAARKLLDILRAMPDGDVALSLNDKRMTVQSGKSRFALQTLAAEEFPTVA
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCCCHHHHHHHHHHCCCCCH
EASEFNASVSLPQKTFKHLLAMVHFAMAQQDIRYYLNGMLLVVDGKKVMAVATDGHRLAY
HHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEECCCEEEE
CGVELENEATGVGSRQEVIIPRKTILELQRLLEDNDDPVQVQLAANQVKFSFANIELISK
EEEEECCCCCCCCCCCEEECCHHHHHHHHHHHCCCCCCEEEEEEECEEEEEEHHHHHHHH
LVEGKFPDFQRVIPKGYKNAFAIDRVRLQQALQRTAILTTDKFKGVRCILDTHMLKISST
HHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEEEEEEEEECC
NADQEEAQEELEIDYSGDALDIGFNVTYLLDVLANLKSEQVQVSLGDSNSSALITVPEDD
CCCHHHHHHHEEEEECCCEEEECCHHHHHHHHHHCCCCCEEEEEECCCCCCEEEECCCCC
NFKYVVMPMRI
CEEEEEEEECC
>Mature Secondary Structure
MQLVKTSRDNLLRPLQIVSGIVERRHTLPILANLLIRKSGSNVSFLSTDIEIQITTHAEC
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEEEEEEEEEECCCC
GVGNDSVATTVAARKLLDILRAMPDGDVALSLNDKRMTVQSGKSRFALQTLAAEEFPTVA
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCCCHHHHHHHHHHCCCCCH
EASEFNASVSLPQKTFKHLLAMVHFAMAQQDIRYYLNGMLLVVDGKKVMAVATDGHRLAY
HHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEECCCEEEE
CGVELENEATGVGSRQEVIIPRKTILELQRLLEDNDDPVQVQLAANQVKFSFANIELISK
EEEEECCCCCCCCCCCEEECCHHHHHHHHHHHCCCCCCEEEEEEECEEEEEEHHHHHHHH
LVEGKFPDFQRVIPKGYKNAFAIDRVRLQQALQRTAILTTDKFKGVRCILDTHMLKISST
HHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEEEEEEEEECC
NADQEEAQEELEIDYSGDALDIGFNVTYLLDVLANLKSEQVQVSLGDSNSSALITVPEDD
CCCHHHHHHHEEEEECCCEEEECCHHHHHHHHHHCCCCCEEEEEECCCCCCEEEECCCCC
NFKYVVMPMRI
CEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]