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The map label for this gene is gyrB [H]

Identifier: 73539709

GI number: 73539709

Start: 3726

End: 6251

Strand: Direct

Name: gyrB [H]

Synonym: Reut_A0003

Alternate gene names: 73539709

Gene position: 3726-6251 (Clockwise)

Preceding gene: 73539708

Following gene: 73539712

Centisome position: 0.1

GC content: 62.03

Gene sequence:

>2526_bases
ATGACCGAACAGCAGAACCCGCAACAGGAAAATACCTACGGAGCCTCTTCGATCCAGATCCTGGAAGGCCTGGAGGCGGT
ACGCAAGCGTCCGGGCATGTACATCGGCGACACCTCCGACGGCACCGGCCTGCACCACCTCGTCTTCGAGGTGCTGGACA
ACTCCATCGACGAAGCGCTGGCCGGCTACTGCACCGAGATCACGGTCACCATCCACAGCGACAACTCGATCTCCATCGTC
GACAACGGCCGCGGCATTCCGCCCCTGGTCAAGTTCGACGACAAGCACGAACCCAAGCGCAGCGCGGCGGAAATCGCCAT
GACCGAGCTGCACGCCGGCGGCAAGTTCAACCAGAACAGCTACAAGGTGTCCGGCGGCCTGCACGGCGTGGGCGTGTCCT
GCGTGAACGCGCTGTCCAAGTGGCTGCGCCTGACCGTGCGCCGTGACGGCAAGGTCCACCTGATCGAATTCGCCAAGGGC
GACGTGCAGAATCGCATCATCGAGACCGTCAACGGCCCGGACGGCCAGCCGGTCGAAGTGTCCCCGATGAAGATCATCGG
CGACACCGACAAGCGTGGCACCGAGGTGCACTTCCTGGCCGACGAGGAAATCTTCACCAACGTCGAGTTCCACTACGAAA
TCCTCTCCAAGCGGATTCGTGAACTCTCGTTCCTGAACAACGGCGTCCACATCAAGCTGGTCGACCAGCGCACCGGCAAG
GAAGAAGACTTCGCCTTCTCCGGCGGCGTGAAGGGCTTTGTCGAGTACATCAACCGCGCCAAGACCGTGCTGCACCCGAA
CATCTTCTACGCGAACGCCGAGAAAGACGGCATTGCGGTGGAAGTGTCGATGCAGTGGAACGACGGCTACAACGAGCAGG
TGCTCTGCTTCACCAACAACATCCCGCAGCGGGATGGCGGCACCCACCTGACCGGCCTGCGCGCCGCGATGACCCGCGTC
ATCAACAAGTACATCGAAGAAAACGAGATCGCCAAGAAGGCCAAGGTCGAAACCAGCGGCGACGACATGCGCGAAGGCCT
GGCCTGCGTGCTGTCCGTCAAGGTGCCCGAGCCCAAGTTCAGCTCGCAGACCAAGGACAAGCTCGTTTCGTCGGAAGTGC
GCCTGCCCGTGGAAGAACTCGTCAGCAAGGCGCTGAACGACTTCCTGCTCGAAACGCCCAACGACGCTAAGACCATCTGC
GGCAAGATCGTCGACGCCGCCCGCGCGCGCGAAGCCGCCCGCAAGGCCCGCGAAATGACGCGCCGCAAGGGCGTCATGGA
CGGCATGGGCCTGCCCGGCAAGCTCGCCGACTGCCAGGAAAAAGACCCGGCCCAGTCCGAACTCTTCCTGGTGGAGGGTG
ACTCCGCAGGCGGCTCCGCCAAGCAGGGCCGCGACCGTAAGTTCCAGGCCATCCTGCCGCTCAAGGGCAAGATCCTGAAC
GTCGAGCGCGCGCGCTTCGACAAGATGCTCTCCAGCCAGGAAGTGCTCACGCTCATCACCGCGCTGGGCACCGGCATCGG
CAAGGACGACTACAACCTGGACAAGCTGCGCTACCACCGCATCATCATCATGACCGACGCGGACGTGGACGGCTCGCACA
TCCGCACGCTGCTGCTGACGTTCTTCTACCGCCAGATGCCCGACATCATCGAGCGCGGCTACGTGTACATCGCCCAGCCG
CCGCTCTACAAGATCAAGCACGGCAAGGAAGAGCGCTACATCAAGGACGACGTCGAACTCAACGCCTACCTGCTCAAGCT
GGCCATGGAAAAGGCCGTGCTCGTGCGCCCGGATGGCACGGAGATCAATGGCGATGCGCTGACCGAGCTGGCGCGCCAGT
ACCAGCTCACGGAAGGCGTGATCGGCCGCCTGTCACGCATCGTGGATACGGATGCGCTGCGCGCGATTGCCGATGGCGTA
ACGCTGGACCTGGACAGCGCACCGGCGGCCGAGGCATCGGCCGTGGCGCTGAAGACCAAGCTGGCCGAGATGCATGCCAA
GACGCTGCTCGGCGCCGCCGTCAACGACGATGGCACGGCGGACGTGTATGCCCAGTTCGACGAAAAGACCGACAAGCATC
GCCTGATGATTGCGCGTCGTCACCATGGCAACGTGCGGCTGTCGCACATGGATGCAGACTTTGTCCACGGGGCGGATTAC
GCGGCTCTGTCCAATGCGGCCAAGACGTTCCAGGGGCTGACGCCTGAGGGGACCAAGGTGCAGCGCGGGGAAGGTGACAA
GATGAGGGATCAGACTGTCAATGACTTCCATGGGGCGATGCAGTGGTTGCTTTCGGAGGCGGAGCGTGGGGTTTCGAGGC
AGCGGTATAAGGGGCTTGGTGAAATGAACCCTGAGCAGCTGTTTGAGACTACCCTCGACGTTACGCAGCGGCGTCTGCTG
AAGGTGCAGATTGAGGATGCTATTGCAGCTGACCAAATCTTTACCACCCTTATGGGGGATGAGGTGGAGCCGCGGAGGAA
CTTTATTGAGAGTAATGCGTTGGTGGCGCGGAATATTGATGTTTAA

Upstream 100 bases:

>100_bases
CCCTTTTGCCGTTTTTAGTAACCCGCCATGCGGAACGCCAGACGCCCTGCCAATGTGCCGGCGCGGCCAGGTTTTCGCAT
CAGCCGTTGAGTAGAAAGAC

Downstream 100 bases:

>100_bases
TCCGTATTGTAGGTTCTCAGAATAGTTGAGAAAAATCACAAGTAAATGACTCAAGGCCAGTCTGCGGGACTGGCTTTGTT
TTGAGTGGGGGAGTAGGGTG

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 841; Mature: 840

Protein sequence:

>841_residues
MTEQQNPQQENTYGASSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSIDEALAGYCTEITVTIHSDNSISIV
DNGRGIPPLVKFDDKHEPKRSAAEIAMTELHAGGKFNQNSYKVSGGLHGVGVSCVNALSKWLRLTVRRDGKVHLIEFAKG
DVQNRIIETVNGPDGQPVEVSPMKIIGDTDKRGTEVHFLADEEIFTNVEFHYEILSKRIRELSFLNNGVHIKLVDQRTGK
EEDFAFSGGVKGFVEYINRAKTVLHPNIFYANAEKDGIAVEVSMQWNDGYNEQVLCFTNNIPQRDGGTHLTGLRAAMTRV
INKYIEENEIAKKAKVETSGDDMREGLACVLSVKVPEPKFSSQTKDKLVSSEVRLPVEELVSKALNDFLLETPNDAKTIC
GKIVDAARAREAARKAREMTRRKGVMDGMGLPGKLADCQEKDPAQSELFLVEGDSAGGSAKQGRDRKFQAILPLKGKILN
VERARFDKMLSSQEVLTLITALGTGIGKDDYNLDKLRYHRIIIMTDADVDGSHIRTLLLTFFYRQMPDIIERGYVYIAQP
PLYKIKHGKEERYIKDDVELNAYLLKLAMEKAVLVRPDGTEINGDALTELARQYQLTEGVIGRLSRIVDTDALRAIADGV
TLDLDSAPAAEASAVALKTKLAEMHAKTLLGAAVNDDGTADVYAQFDEKTDKHRLMIARRHHGNVRLSHMDADFVHGADY
AALSNAAKTFQGLTPEGTKVQRGEGDKMRDQTVNDFHGAMQWLLSEAERGVSRQRYKGLGEMNPEQLFETTLDVTQRRLL
KVQIEDAIAADQIFTTLMGDEVEPRRNFIESNALVARNIDV

Sequences:

>Translated_841_residues
MTEQQNPQQENTYGASSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSIDEALAGYCTEITVTIHSDNSISIV
DNGRGIPPLVKFDDKHEPKRSAAEIAMTELHAGGKFNQNSYKVSGGLHGVGVSCVNALSKWLRLTVRRDGKVHLIEFAKG
DVQNRIIETVNGPDGQPVEVSPMKIIGDTDKRGTEVHFLADEEIFTNVEFHYEILSKRIRELSFLNNGVHIKLVDQRTGK
EEDFAFSGGVKGFVEYINRAKTVLHPNIFYANAEKDGIAVEVSMQWNDGYNEQVLCFTNNIPQRDGGTHLTGLRAAMTRV
INKYIEENEIAKKAKVETSGDDMREGLACVLSVKVPEPKFSSQTKDKLVSSEVRLPVEELVSKALNDFLLETPNDAKTIC
GKIVDAARAREAARKAREMTRRKGVMDGMGLPGKLADCQEKDPAQSELFLVEGDSAGGSAKQGRDRKFQAILPLKGKILN
VERARFDKMLSSQEVLTLITALGTGIGKDDYNLDKLRYHRIIIMTDADVDGSHIRTLLLTFFYRQMPDIIERGYVYIAQP
PLYKIKHGKEERYIKDDVELNAYLLKLAMEKAVLVRPDGTEINGDALTELARQYQLTEGVIGRLSRIVDTDALRAIADGV
TLDLDSAPAAEASAVALKTKLAEMHAKTLLGAAVNDDGTADVYAQFDEKTDKHRLMIARRHHGNVRLSHMDADFVHGADY
AALSNAAKTFQGLTPEGTKVQRGEGDKMRDQTVNDFHGAMQWLLSEAERGVSRQRYKGLGEMNPEQLFETTLDVTQRRLL
KVQIEDAIAADQIFTTLMGDEVEPRRNFIESNALVARNIDV
>Mature_840_residues
TEQQNPQQENTYGASSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSIDEALAGYCTEITVTIHSDNSISIVD
NGRGIPPLVKFDDKHEPKRSAAEIAMTELHAGGKFNQNSYKVSGGLHGVGVSCVNALSKWLRLTVRRDGKVHLIEFAKGD
VQNRIIETVNGPDGQPVEVSPMKIIGDTDKRGTEVHFLADEEIFTNVEFHYEILSKRIRELSFLNNGVHIKLVDQRTGKE
EDFAFSGGVKGFVEYINRAKTVLHPNIFYANAEKDGIAVEVSMQWNDGYNEQVLCFTNNIPQRDGGTHLTGLRAAMTRVI
NKYIEENEIAKKAKVETSGDDMREGLACVLSVKVPEPKFSSQTKDKLVSSEVRLPVEELVSKALNDFLLETPNDAKTICG
KIVDAARAREAARKAREMTRRKGVMDGMGLPGKLADCQEKDPAQSELFLVEGDSAGGSAKQGRDRKFQAILPLKGKILNV
ERARFDKMLSSQEVLTLITALGTGIGKDDYNLDKLRYHRIIIMTDADVDGSHIRTLLLTFFYRQMPDIIERGYVYIAQPP
LYKIKHGKEERYIKDDVELNAYLLKLAMEKAVLVRPDGTEINGDALTELARQYQLTEGVIGRLSRIVDTDALRAIADGVT
LDLDSAPAAEASAVALKTKLAEMHAKTLLGAAVNDDGTADVYAQFDEKTDKHRLMIARRHHGNVRLSHMDADFVHGADYA
ALSNAAKTFQGLTPEGTKVQRGEGDKMRDQTVNDFHGAMQWLLSEAERGVSRQRYKGLGEMNPEQLFETTLDVTQRRLLK
VQIEDAIAADQIFTTLMGDEVEPRRNFIESNALVARNIDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=601, Percent_Identity=24.6256239600666, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI19913406, Length=561, Percent_Identity=24.0641711229947, Blast_Score=102, Evalue=2e-21,
Organism=Escherichia coli, GI48994957, Length=837, Percent_Identity=58.6618876941458, Blast_Score=930, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=584, Percent_Identity=38.8698630136986, Blast_Score=383, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17535065, Length=604, Percent_Identity=25.6622516556291, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI212645845, Length=534, Percent_Identity=22.4719101123595, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI212645657, Length=155, Percent_Identity=36.1290322580645, Blast_Score=78, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=613, Percent_Identity=23.0016313213703, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI17136538, Length=561, Percent_Identity=24.4206773618538, Blast_Score=106, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 93367; Mature: 93236

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQQNPQQENTYGASSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSIDEAL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHH
AGYCTEITVTIHSDNSISIVDNGRGIPPLVKFDDKHEPKRSAAEIAMTELHAGGKFNQNS
HCCEEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
YKVSGGLHGVGVSCVNALSKWLRLTVRRDGKVHLIEFAKGDVQNRIIETVNGPDGQPVEV
EEECCCCCCHHHHHHHHHHHHHEEHEECCCCEEEEEECCCCHHHHHHHHCCCCCCCCEEE
SPMKIIGDTDKRGTEVHFLADEEIFTNVEFHYEILSKRIRELSFLNNGVHIKLVDQRTGK
CCEEEECCCCCCCCEEEEEECCHHHHCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
EEDFAFSGGVKGFVEYINRAKTVLHPNIFYANAEKDGIAVEVSMQWNDGYNEQVLCFTNN
CCCCEECCCHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEEEECCCCCCEEEEEECC
IPQRDGGTHLTGLRAAMTRVINKYIEENEIAKKAKVETSGDDMREGLACVLSVKVPEPKF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCEEEEEEEECCCCCC
SSQTKDKLVSSEVRLPVEELVSKALNDFLLETPNDAKTICGKIVDAARAREAARKAREMT
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RRKGVMDGMGLPGKLADCQEKDPAQSELFLVEGDSAGGSAKQGRDRKFQAILPLKGKILN
HHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEEE
VERARFDKMLSSQEVLTLITALGTGIGKDDYNLDKLRYHRIIIMTDADVDGSHIRTLLLT
HHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHH
FFYRQMPDIIERGYVYIAQPPLYKIKHGKEERYIKDDVELNAYLLKLAMEKAVLVRPDGT
HHHHHHHHHHHCCEEEEECCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHCEEEECCCCC
EINGDALTELARQYQLTEGVIGRLSRIVDTDALRAIADGVTLDLDSAPAAEASAVALKTK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHH
LAEMHAKTLLGAAVNDDGTADVYAQFDEKTDKHRLMIARRHHGNVRLSHMDADFVHGADY
HHHHHHHHHHHHCCCCCCCCHHEEECCCCCCCCEEEEEEECCCCEEEEECCCHHHCCCCH
AALSNAAKTFQGLTPEGTKVQRGEGDKMRDQTVNDFHGAMQWLLSEAERGVSRQRYKGLG
HHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCC
EMNPEQLFETTLDVTQRRLLKVQIEDAIAADQIFTTLMGDEVEPRRNFIESNALVARNID
CCCHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEECCC
V
C
>Mature Secondary Structure 
TEQQNPQQENTYGASSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSIDEAL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHH
AGYCTEITVTIHSDNSISIVDNGRGIPPLVKFDDKHEPKRSAAEIAMTELHAGGKFNQNS
HCCEEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
YKVSGGLHGVGVSCVNALSKWLRLTVRRDGKVHLIEFAKGDVQNRIIETVNGPDGQPVEV
EEECCCCCCHHHHHHHHHHHHHEEHEECCCCEEEEEECCCCHHHHHHHHCCCCCCCCEEE
SPMKIIGDTDKRGTEVHFLADEEIFTNVEFHYEILSKRIRELSFLNNGVHIKLVDQRTGK
CCEEEECCCCCCCCEEEEEECCHHHHCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
EEDFAFSGGVKGFVEYINRAKTVLHPNIFYANAEKDGIAVEVSMQWNDGYNEQVLCFTNN
CCCCEECCCHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEEEECCCCCCEEEEEECC
IPQRDGGTHLTGLRAAMTRVINKYIEENEIAKKAKVETSGDDMREGLACVLSVKVPEPKF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCEEEEEEEECCCCCC
SSQTKDKLVSSEVRLPVEELVSKALNDFLLETPNDAKTICGKIVDAARAREAARKAREMT
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RRKGVMDGMGLPGKLADCQEKDPAQSELFLVEGDSAGGSAKQGRDRKFQAILPLKGKILN
HHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEEE
VERARFDKMLSSQEVLTLITALGTGIGKDDYNLDKLRYHRIIIMTDADVDGSHIRTLLLT
HHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHH
FFYRQMPDIIERGYVYIAQPPLYKIKHGKEERYIKDDVELNAYLLKLAMEKAVLVRPDGT
HHHHHHHHHHHCCEEEEECCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHCEEEECCCCC
EINGDALTELARQYQLTEGVIGRLSRIVDTDALRAIADGVTLDLDSAPAAEASAVALKTK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHH
LAEMHAKTLLGAAVNDDGTADVYAQFDEKTDKHRLMIARRHHGNVRLSHMDADFVHGADY
HHHHHHHHHHHHCCCCCCCCHHEEECCCCCCCCEEEEEEECCCCEEEEECCCHHHCCCCH
AALSNAAKTFQGLTPEGTKVQRGEGDKMRDQTVNDFHGAMQWLLSEAERGVSRQRYKGLG
HHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCC
EMNPEQLFETTLDVTQRRLLKVQIEDAIAADQIFTTLMGDEVEPRRNFIESNALVARNID
CCCHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEECCC
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1906260 [H]