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The map label for this gene is hemK [H]

Identifier: 71910677

GI number: 71910677

Start: 854250

End: 855089

Strand: Direct

Name: hemK [H]

Synonym: M5005_Spy_0864

Alternate gene names: 71910677

Gene position: 854250-855089 (Clockwise)

Preceding gene: 71910676

Following gene: 71910678

Centisome position: 46.46

GC content: 35.95

Gene sequence:

>840_bases
ATGAACTATGCTACATTAATCAGAACTTATGAAGATAAACTAGAACAAATAGATGAGGATCGCGAAAATCTCGCCTATGT
TTTTAGAGAAATCAAAGAATGGTCAAGTCTAGATATGTTGATTCACCAAAATCAAGCTGTGACTCCTGAAGATGCTGTTC
TTTTGGAACATATTTTCTGTTCTCTAAGTCAGCATTTATCTCCTCAATATATTACAGGTAATGCTTATTTTCGTGATTTG
AAGCTTGCAGTCGACAAGCGTGTATTAATTCCAAGGCCAGAAACAGAAGAATTAGTTGACATGATTTTAGCTGAAAATCT
TGATGCACCGTTGAATGTTTTAGATATTGGTACAGGTAGTGGAGCGATTGCTATTTCTCTCAAAAAAGAAAGGCCTAACT
GGCAAGTAACGGCATCTGATATTTCTAGAGCTGCTCTTGACTTGGCAAAAGCCAATGCTGATGCTTACCAGCTTGATATA
ACGTTTATTGAATCAGATGTTTTTAGTCTCATTTCAGAAACATTTGATATTATTGTGTCAAATCCTCCCTACATATCCTA
CGAGGATAAGGAAGAAGTCAGTCTAAATGTTTTGCAATCAGAGCCTCATTTGGCACTTTTTGCTAAAGAAAATGGCTATG
CTATTTACCGTAAGATTATTGAACAAGCTGACAATTACTTAACTAAGGAGGGGAAGCTTTACTTTGAAATTGGTTATAAG
CAAGCTGAAGGTATAAAAGATATGTTACAAGCCTATTTTCCTCAAAGACATATACGAGCCGTAACGGATATATTTGGCAA
AGAAAGGATGGTGGTTGTTGATTATGGAGTATCTAGCTAG

Upstream 100 bases:

>100_bases
TTCAAAAATTAGATACTATTTTATCTGGAAAAATGGATGAAGTTATTGATGCACTCGTTATGTATGATCAGACAAAGAAA
TTAGAATCTCTCAATAACTA

Downstream 100 bases:

>100_bases
TATCATTGAATCAGGTGATGCCCTTGTTTTACCAACAGAAACGGTTTATGGTCTTTTTGCCAAGGCTTTAGATGAAAAAG
CAGTAAATGCTGTTTATGAC

Product: peptide release factor-glutamine N5-methyltransferase

Products: NA

Alternate protein names: HemK protein homolog; M.RcoHemKP; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MNYATLIRTYEDKLEQIDEDRENLAYVFREIKEWSSLDMLIHQNQAVTPEDAVLLEHIFCSLSQHLSPQYITGNAYFRDL
KLAVDKRVLIPRPETEELVDMILAENLDAPLNVLDIGTGSGAIAISLKKERPNWQVTASDISRAALDLAKANADAYQLDI
TFIESDVFSLISETFDIIVSNPPYISYEDKEEVSLNVLQSEPHLALFAKENGYAIYRKIIEQADNYLTKEGKLYFEIGYK
QAEGIKDMLQAYFPQRHIRAVTDIFGKERMVVVDYGVSS

Sequences:

>Translated_279_residues
MNYATLIRTYEDKLEQIDEDRENLAYVFREIKEWSSLDMLIHQNQAVTPEDAVLLEHIFCSLSQHLSPQYITGNAYFRDL
KLAVDKRVLIPRPETEELVDMILAENLDAPLNVLDIGTGSGAIAISLKKERPNWQVTASDISRAALDLAKANADAYQLDI
TFIESDVFSLISETFDIIVSNPPYISYEDKEEVSLNVLQSEPHLALFAKENGYAIYRKIIEQADNYLTKEGKLYFEIGYK
QAEGIKDMLQAYFPQRHIRAVTDIFGKERMVVVDYGVSS
>Mature_279_residues
MNYATLIRTYEDKLEQIDEDRENLAYVFREIKEWSSLDMLIHQNQAVTPEDAVLLEHIFCSLSQHLSPQYITGNAYFRDL
KLAVDKRVLIPRPETEELVDMILAENLDAPLNVLDIGTGSGAIAISLKKERPNWQVTASDISRAALDLAKANADAYQLDI
TFIESDVFSLISETFDIIVSNPPYISYEDKEEVSLNVLQSEPHLALFAKENGYAIYRKIIEQADNYLTKEGKLYFEIGYK
QAEGIKDMLQAYFPQRHIRAVTDIFGKERMVVVDYGVSS

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=255, Percent_Identity=32.156862745098, Blast_Score=90, Evalue=2e-18,
Organism=Escherichia coli, GI1787463, Length=179, Percent_Identity=41.340782122905, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI87082085, Length=189, Percent_Identity=34.3915343915344, Blast_Score=105, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6324266, Length=189, Percent_Identity=30.6878306878307, Blast_Score=73, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24582226, Length=284, Percent_Identity=28.169014084507, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 31826; Mature: 31826

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYATLIRTYEDKLEQIDEDRENLAYVFREIKEWSSLDMLIHQNQAVTPEDAVLLEHIFC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHH
SLSQHLSPQYITGNAYFRDLKLAVDKRVLIPRPETEELVDMILAENLDAPLNVLDIGTGS
HHHHCCCCCEEECCHHHHHHHHHHCCEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCC
GAIAISLKKERPNWQVTASDISRAALDLAKANADAYQLDITFIESDVFSLISETFDIIVS
CEEEEEEECCCCCEEEEHHHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHHHHHHEEEC
NPPYISYEDKEEVSLNVLQSEPHLALFAKENGYAIYRKIIEQADNYLTKEGKLYFEIGYK
CCCEECCCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCC
QAEGIKDMLQAYFPQRHIRAVTDIFGKERMVVVDYGVSS
HHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCCCC
>Mature Secondary Structure
MNYATLIRTYEDKLEQIDEDRENLAYVFREIKEWSSLDMLIHQNQAVTPEDAVLLEHIFC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHH
SLSQHLSPQYITGNAYFRDLKLAVDKRVLIPRPETEELVDMILAENLDAPLNVLDIGTGS
HHHHCCCCCEEECCHHHHHHHHHHCCEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCC
GAIAISLKKERPNWQVTASDISRAALDLAKANADAYQLDITFIESDVFSLISETFDIIVS
CEEEEEEECCCCCEEEEHHHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHHHHHHEEEC
NPPYISYEDKEEVSLNVLQSEPHLALFAKENGYAIYRKIIEQADNYLTKEGKLYFEIGYK
CCCEECCCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCC
QAEGIKDMLQAYFPQRHIRAVTDIFGKERMVVVDYGVSS
HHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]