Click here to switch to the map view.

The map label for this gene is prfA [H]

Identifier: 71910676

GI number: 71910676

Start: 853171

End: 854250

Strand: Direct

Name: prfA [H]

Synonym: M5005_Spy_0863

Alternate gene names: 71910676

Gene position: 853171-854250 (Clockwise)

Preceding gene: 71910675

Following gene: 71910677

Centisome position: 46.4

GC content: 37.41

Gene sequence:

>1080_bases
ATGAATATTTATGATCAATTGCAGGCAGTTGAAGATCGTTATGAAGAGTTAGGTGAGTTACTTAGTGATCCAGATGTTGT
CTCAGATACAAAGCGATTTATGGAATTATCACGTGAAGAAGCTAATACTCGAGAAACTGTCACAGCTTATAGAGAATATA
AGCAAGTCATTCAAACCATTTCAGATGCTGAAGAAATGATCAAAGATGCCAGTGGTGATCCTGAATTAGAAGAAATGGCT
AAAGAAGAACTTAAAGAATCAAAAGCAGCTAAAGAAGAGTATGAAGAAAAATTAAAAATCCTTCTATTACCTAAAGATCC
TAACGATGATAAAAACATTATTTTAGAAATTCGTGGTGCTGCTGGTGGCGATGAAGCAGCGCTTTTTGCTGGTGATCTTT
TAACAATGTATCAAAAATATGCTGAAACTCAAGGATGGCGTTTTGAAGTTATGGAATCATCCGTTAATGGTGTTGGTGGT
ATTAAAGAAGTGGTTGCCATGGTTTCTGGTCAGTCAGTTTATTCTAAGCTTAAATATGAATCAGGAGCTCATCGTGTTCA
ACGTGTCCCAGTGACAGAGAGTCAGGGTCGTGTTCATACTTCAACTGCAACTGTTTTGGTAATGCCAGAAGTTGAAGAAG
TAGAATACGACATTGATCCTAAAGACCTTCGCGTCGATATTTACCACGCGTCTGGTGCTGGTGGTCAGAATGTCAATAAA
GTTGCTACTGCAGTTCGTATGGTACACATTCCTACAGGAATCAAAGTTGAAATGCAAGAAGAGCGTACTCAGCAAAAAAA
CCGTGATAAAGCGATGAAAATCATTCGTGCTCGTGTTGCTGACCATTTTGCGCAAATTGCTCAAGATGAACAAGATGCTG
AACGTAAATCAACAGTTGGTACTGGTGATCGTTCAGAACGTATTCGTACTTATAATTTCCCGCAAAATCGTGTAACAGAT
CATCGTATTGGTTTGACACTTCAAAAATTAGATACTATTTTATCTGGAAAAATGGATGAAGTTATTGATGCACTCGTTAT
GTATGATCAGACAAAGAAATTAGAATCTCTCAATAACTAA

Upstream 100 bases:

>100_bases
CAAATTGGTGGTAATGAAACATATATCCCAGTTTGTCGAAAACATTATTTTAATCCAGATATTTAAAAGGTAATCGGTTT
TAAAGGAGATACAAAGAAAA

Downstream 100 bases:

>100_bases
TGAACTATGCTACATTAATCAGAACTTATGAAGATAAACTAGAACAAATAGATGAGGATCGCGAAAATCTCGCCTATGTT
TTTAGAGAAATCAAAGAATG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTRETVTAYREYKQVIQTISDAEEMIKDASGDPELEEMA
KEELKESKAAKEEYEEKLKILLLPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDIDPKDLRVDIYHASGAGGQNVNK
VATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIRARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTD
HRIGLTLQKLDTILSGKMDEVIDALVMYDQTKKLESLNN

Sequences:

>Translated_359_residues
MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTRETVTAYREYKQVIQTISDAEEMIKDASGDPELEEMA
KEELKESKAAKEEYEEKLKILLLPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDIDPKDLRVDIYHASGAGGQNVNK
VATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIRARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTD
HRIGLTLQKLDTILSGKMDEVIDALVMYDQTKKLESLNN
>Mature_359_residues
MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTRETVTAYREYKQVIQTISDAEEMIKDASGDPELEEMA
KEELKESKAAKEEYEEKLKILLLPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDIDPKDLRVDIYHASGAGGQNVNK
VATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIRARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTD
HRIGLTLQKLDTILSGKMDEVIDALVMYDQTKKLESLNN

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=292, Percent_Identity=43.1506849315069, Blast_Score=254, Evalue=7e-68,
Organism=Homo sapiens, GI34577120, Length=349, Percent_Identity=37.5358166189112, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI166795305, Length=196, Percent_Identity=41.3265306122449, Blast_Score=173, Evalue=2e-43,
Organism=Escherichia coli, GI1787462, Length=360, Percent_Identity=49.1666666666667, Blast_Score=354, Evalue=5e-99,
Organism=Escherichia coli, GI2367172, Length=349, Percent_Identity=35.5300859598854, Blast_Score=194, Evalue=7e-51,
Organism=Caenorhabditis elegans, GI17542784, Length=288, Percent_Identity=38.8888888888889, Blast_Score=188, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6321295, Length=318, Percent_Identity=42.1383647798742, Blast_Score=248, Evalue=1e-66,
Organism=Drosophila melanogaster, GI19921226, Length=344, Percent_Identity=37.2093023255814, Blast_Score=221, Evalue=5e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40569; Mature: 40569

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTRETVTAYREYKQVIQTI
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
SDAEEMIKDASGDPELEEMAKEELKESKAAKEEYEEKLKILLLPKDPNDDKNIILEIRGA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEECC
AGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGGIKEVVAMVSGQSVYSKLKYE
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCHHHHHHHHHCCHHHHHHHHHC
SGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDIDPKDLRVDIYHASGAGGQNVNK
CCCHHHEECCCCCCCCEEEECCEEEEEECCHHHCCCCCCCCCEEEEEEEECCCCCCCHHH
VATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIRARVADHFAQIAQDEQDAERKSTVG
HHHHHHHEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
TGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTILSGKMDEVIDALVMYDQTKKLESLNN
CCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTRETVTAYREYKQVIQTI
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
SDAEEMIKDASGDPELEEMAKEELKESKAAKEEYEEKLKILLLPKDPNDDKNIILEIRGA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEECC
AGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGGIKEVVAMVSGQSVYSKLKYE
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCHHHHHHHHHCCHHHHHHHHHC
SGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDIDPKDLRVDIYHASGAGGQNVNK
CCCHHHEECCCCCCCCEEEECCEEEEEECCHHHCCCCCCCCCEEEEEEEECCCCCCCHHH
VATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIRARVADHFAQIAQDEQDAERKSTVG
HHHHHHHEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
TGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTILSGKMDEVIDALVMYDQTKKLESLNN
CCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA