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The map label for this gene is mscL [H]

Identifier: 71910411

GI number: 71910411

Start: 600736

End: 601098

Strand: Reverse

Name: mscL [H]

Synonym: M5005_Spy_0598

Alternate gene names: 71910411

Gene position: 601098-600736 (Counterclockwise)

Preceding gene: 71910429

Following gene: 71910406

Centisome position: 32.69

GC content: 35.26

Gene sequence:

>363_bases
ATGGTTAAAGAATTAAAAGCATTTTTGTTTAGAGGAAACATTATTGAACTTGCTGTAGCAGTTATCATCGGGGGAGCTTT
TGGTGCTATTGTCACTTCTTTTGTGAACGATATTATCACACCGCTTATTTTAAACCCAGCTTTGAAAGCTGCAAATGTTG
AAAACATCACTCAATTGTCATGGAATGGTGTTAAATATGGTAGCTTTTTAGGAGCCGTTATTAATTTTCTTATCATCGGT
ACCAGTCTTTTCTTTGTTGTAAAAGCCGCTGAAAAAGCTATGCCTAAAAAGGAAAAAGAAGCTGCAGCTCCTACTCAAGA
AGAATTATTGACAGAAATCCGTGATTTGTTGGCTCAAAAATAA

Upstream 100 bases:

>100_bases
ACTCTTTTTTGTAAATTTAAAAACTGAAGTGATTCCATTGGATTTTGTTTACTAATTATGCTATGCTTAACTTAGAGATA
TTAAAAAGGGGGAATGTTTA

Downstream 100 bases:

>100_bases
AGCGATTTTTGATAAAGTAGTTAGGCATGTCTAACTACTTTTTTTGATATCAAAAAAGCTCCCTTGCTTCTTCTGGATAA
AAAATAAAAACAGTTCTAAT

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 120; Mature: 120

Protein sequence:

>120_residues
MVKELKAFLFRGNIIELAVAVIIGGAFGAIVTSFVNDIITPLILNPALKAANVENITQLSWNGVKYGSFLGAVINFLIIG
TSLFFVVKAAEKAMPKKEKEAAAPTQEELLTEIRDLLAQK

Sequences:

>Translated_120_residues
MVKELKAFLFRGNIIELAVAVIIGGAFGAIVTSFVNDIITPLILNPALKAANVENITQLSWNGVKYGSFLGAVINFLIIG
TSLFFVVKAAEKAMPKKEKEAAAPTQEELLTEIRDLLAQK
>Mature_120_residues
MVKELKAFLFRGNIIELAVAVIIGGAFGAIVTSFVNDIITPLILNPALKAANVENITQLSWNGVKYGSFLGAVINFLIIG
TSLFFVVKAAEKAMPKKEKEAAAPTQEELLTEIRDLLAQK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=133, Percent_Identity=42.1052631578947, Blast_Score=84, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 12961; Mature: 12961

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKELKAFLFRGNIIELAVAVIIGGAFGAIVTSFVNDIITPLILNPALKAANVENITQLS
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHC
WNGVKYGSFLGAVINFLIIGTSLFFVVKAAEKAMPKKEKEAAAPTQEELLTEIRDLLAQK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVKELKAFLFRGNIIELAVAVIIGGAFGAIVTSFVNDIITPLILNPALKAANVENITQLS
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHC
WNGVKYGSFLGAVINFLIIGTSLFFVVKAAEKAMPKKEKEAAAPTQEELLTEIRDLLAQK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA