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The map label for this gene is ubiE

Identifier: 71892390

GI number: 71892390

Start: 777805

End: 778569

Strand: Reverse

Name: ubiE

Synonym: BPEN_649

Alternate gene names: 71892390

Gene position: 778569-777805 (Counterclockwise)

Preceding gene: 71892391

Following gene: 71892389

Centisome position: 98.35

GC content: 29.28

Gene sequence:

>765_bases
ATGAATATGAAGAATAAATCTGAAAAAGAAATGACTCATTTTGGTTTTCAAAATGTTTACAAAGATAAGAAAACATCATT
AGTGTCTAATGTATTCCATACTGTAGCATCACAGTATGATTTAATGAATGATTTAATGTCGTTTGGAATACATAGAATGT
GGAAACGATTTACGATTTATCGCAGCGAAGTATATGTTGGATGCATTGTATTGGATCTAGCTGGAGGTACTGGAGATTTA
AGTATTCAATTTTCCCGGTTAGTAGGTAATACAGGGATCGTAGTGCTAGCAGATATCAATGATTCCATGCTACATATAGG
GCAAAAAAAATTACGCGATTTAGGTATATTAAATAATGTAATATATATACAAGCTGACGCTGAAGCTTTACCTTTCTCTG
AAAATACTTTTGATTGCGTTGCTGTTTCTTTTGGATTACGCAATTTTACAAATAAAGAACAAGCATTATTTTCAATACAT
AGAGTATTAAAACCTAGAGGAAAATTATTAATTTTAGATTTCGGAGTGCCTAATTTTAAAGTATTGCACAAAATATATGA
TTTATATTCATTTCATATTTTACCTAAAATAGGTAAATGTATAACTCAAGATATTAATAGTTATCGTTATCTTGTAGAAT
CTATTCGTATGCATCCAGATCAAGAAACTTTAAAAAACATGATAATACGAACAGGATTCAGCAATGTTGAATATTTCAAT
ATGACTTTTGGGATTGCTGTATTACATTGCGCTTATAAATGTTAA

Upstream 100 bases:

>100_bases
ACATTAAAGTATGTGACTTAATAAAATCAAAGTACTATTTTAGTAAAATTAGTATTATTTTTTGATGAATAGAAATGTAA
TGATAAATATTACGGTAATG

Downstream 100 bases:

>100_bases
AAAAATATTAATATTCAGTGAATTATTAAAATAGACAGTGTCATTATTTTGTTAACATCGCCAAGAACGTGAATATTTAT
TAAATCCTGTAAAGATATTT

Product: 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MNMKNKSEKEMTHFGFQNVYKDKKTSLVSNVFHTVASQYDLMNDLMSFGIHRMWKRFTIYRSEVYVGCIVLDLAGGTGDL
SIQFSRLVGNTGIVVLADINDSMLHIGQKKLRDLGILNNVIYIQADAEALPFSENTFDCVAVSFGLRNFTNKEQALFSIH
RVLKPRGKLLILDFGVPNFKVLHKIYDLYSFHILPKIGKCITQDINSYRYLVESIRMHPDQETLKNMIIRTGFSNVEYFN
MTFGIAVLHCAYKC

Sequences:

>Translated_254_residues
MNMKNKSEKEMTHFGFQNVYKDKKTSLVSNVFHTVASQYDLMNDLMSFGIHRMWKRFTIYRSEVYVGCIVLDLAGGTGDL
SIQFSRLVGNTGIVVLADINDSMLHIGQKKLRDLGILNNVIYIQADAEALPFSENTFDCVAVSFGLRNFTNKEQALFSIH
RVLKPRGKLLILDFGVPNFKVLHKIYDLYSFHILPKIGKCITQDINSYRYLVESIRMHPDQETLKNMIIRTGFSNVEYFN
MTFGIAVLHCAYKC
>Mature_254_residues
MNMKNKSEKEMTHFGFQNVYKDKKTSLVSNVFHTVASQYDLMNDLMSFGIHRMWKRFTIYRSEVYVGCIVLDLAGGTGDL
SIQFSRLVGNTGIVVLADINDSMLHIGQKKLRDLGILNNVIYIQADAEALPFSENTFDCVAVSFGLRNFTNKEQALFSIH
RVLKPRGKLLILDFGVPNFKVLHKIYDLYSFHILPKIGKCITQDINSYRYLVESIRMHPDQETLKNMIIRTGFSNVEYFN
MTFGIAVLHCAYKC

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2)

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family

Homologues:

Organism=Homo sapiens, GI116063536, Length=275, Percent_Identity=38.9090909090909, Blast_Score=211, Evalue=7e-55,
Organism=Escherichia coli, GI48994983, Length=251, Percent_Identity=62.1513944223108, Blast_Score=337, Evalue=5e-94,
Organism=Caenorhabditis elegans, GI17557039, Length=253, Percent_Identity=42.6877470355731, Blast_Score=202, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6323526, Length=264, Percent_Identity=36.7424242424242, Blast_Score=164, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24641702, Length=259, Percent_Identity=38.6100386100386, Blast_Score=204, Evalue=5e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIE_BLOPB (Q491V7)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_278124.1
- ProteinModelPortal:   Q491V7
- STRING:   Q491V7
- GeneID:   3562726
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_649
- eggNOG:   COG2226
- HOGENOM:   HBG559580
- OMA:   FDVYTIA
- ProtClustDB:   CLSK280961
- BioCyc:   CBLO291272:BPEN_649-MONOMER
- HAMAP:   MF_01813
- InterPro:   IPR004033
- PANTHER:   PTHR10108:SF24
- TIGRFAMs:   TIGR01934

Pfam domain/function: PF01209 Ubie_methyltran

EC number: 2.1.1.-

Molecular weight: Translated: 29131; Mature: 29131

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS01183 UBIE_1; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMKNKSEKEMTHFGFQNVYKDKKTSLVSNVFHTVASQYDLMNDLMSFGIHRMWKRFTIY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSEVYVGCIVLDLAGGTGDLSIQFSRLVGNTGIVVLADINDSMLHIGQKKLRDLGILNNV
HHHHEEEEEEEEECCCCCCEEEEEEHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCE
IYIQADAEALPFSENTFDCVAVSFGLRNFTNKEQALFSIHRVLKPRGKLLILDFGVPNFK
EEEEECCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHH
VLHKIYDLYSFHILPKIGKCITQDINSYRYLVESIRMHPDQETLKNMIIRTGFSNVEYFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEH
MTFGIAVLHCAYKC
HHHHHHHHHHHHCC
>Mature Secondary Structure
MNMKNKSEKEMTHFGFQNVYKDKKTSLVSNVFHTVASQYDLMNDLMSFGIHRMWKRFTIY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSEVYVGCIVLDLAGGTGDLSIQFSRLVGNTGIVVLADINDSMLHIGQKKLRDLGILNNV
HHHHEEEEEEEEECCCCCCEEEEEEHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCE
IYIQADAEALPFSENTFDCVAVSFGLRNFTNKEQALFSIHRVLKPRGKLLILDFGVPNFK
EEEEECCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHH
VLHKIYDLYSFHILPKIGKCITQDINSYRYLVESIRMHPDQETLKNMIIRTGFSNVEYFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEH
MTFGIAVLHCAYKC
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA