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The map label for this gene is nifS [H]

Identifier: 71892307

GI number: 71892307

Start: 662989

End: 664158

Strand: Reverse

Name: nifS [H]

Synonym: BPEN_554

Alternate gene names: 71892307

Gene position: 664158-662989 (Counterclockwise)

Preceding gene: 71892309

Following gene: 71892306

Centisome position: 83.89

GC content: 34.53

Gene sequence:

>1170_bases
ATGAAGTTACCAATATATTTTGACTATGCTGCTACTACTCCCGTAGACCCTAGAGTAGTAGAAAAAATGATACCATATTT
TACCCTAGACGGCATATTTGGTAACCCGTCTTCTCGTTCTCATTATTATGGCTGGAAAGCAGAGGAAGCGGTAGATATAG
CTCGTAAACAAATCGCTCAGTTGATAGGTGCTGAATCACGTGAAATCATTTTTACTTCAGGAGCTACTGAATCAGTAAAT
TTAGCTATTAAAGGAATAGCTCAATCTTATCACAAAAAAGGAAAACATATTGTTACCAGCATGACGGAACACAAATCAGT
TTTAGATACTTGTCAATATCTTGAGAACAAAGGATTTGAAATTACCCGTATAACTCCACAGCCCAATGGTTTAATCACTT
TCGAACAAATTAGTAACGCACTACGTGATGATACAATTTTATTATCTATTATGCATGTAAATAATGAAATCGGTGTAATC
CAAGACATCAAAAAATTTGGTAAATTATGTCGTCTACATGACATAATATTTCATGTAGACGCAACTCAAAGCGTTGGTAA
ATTACATATTGATTTATCTGATTTACCGGTGGATTTAATGTCTTTCAACGGCCATAAACTATATGGCCCAAAAGGTATCG
GTGGTTTGTACATACGACAACATAGGCATCCAAAACTCCATATTACAGCTCAAATGCATGGAGGAGGACATGAACACGGT
ATGCGTTCTGGTACATTACCAGTCCATCAAATTGTTGGTATGGCTGAAGCGTATCGTTTGGCTAGAGAAACAATGACGAC
AGAAATGATTTATTTAAAAAAAATGAGAGATCGTCTATGGCAAGGATTACAACAAATTGAAGGAATATTAATTAATGGCG
ATTTAGAGTATAGTATAGGCACATTGTTGAATATTAGTTTTAATAATATTGACGGTGAAACATTAATTATAGCATTAAAA
GATTTTGCATTATCTTCTGGGTCTGCTTGTACCTCTGGCACTTTAAATCCATCCCATGTACTGCAGGCTTTAGGAAGAAA
TAATGAACTAGCATATAATTCAATCAGATTTTCTTTAGGGCGTTTCACAACTGAAGAAGAAATAGAGTACGCTATCCATC
ATATAGCTGCTACTGTTATGAAATTAAATGCTATGATATATTTATTATAA

Upstream 100 bases:

>100_bases
TTTTAGAGCATTCAATTTAATATATTATATAAATGAGCTGATTTTATAATTTTTATATTTTATACATCAAAATAAGTTTT
TTATTTTTGGAGTATGTTAG

Downstream 100 bases:

>100_bases
TTTTACAATAAAAAATAAATTAATTTGTTTTTCATATATGAAGTTTATGACGATAATCAATATATCTATAAAAAAATCTA
AGAAATTTTACTAATATTAT

Product: putative L-cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MKLPIYFDYAATTPVDPRVVEKMIPYFTLDGIFGNPSSRSHYYGWKAEEAVDIARKQIAQLIGAESREIIFTSGATESVN
LAIKGIAQSYHKKGKHIVTSMTEHKSVLDTCQYLENKGFEITRITPQPNGLITFEQISNALRDDTILLSIMHVNNEIGVI
QDIKKFGKLCRLHDIIFHVDATQSVGKLHIDLSDLPVDLMSFNGHKLYGPKGIGGLYIRQHRHPKLHITAQMHGGGHEHG
MRSGTLPVHQIVGMAEAYRLARETMTTEMIYLKKMRDRLWQGLQQIEGILINGDLEYSIGTLLNISFNNIDGETLIIALK
DFALSSGSACTSGTLNPSHVLQALGRNNELAYNSIRFSLGRFTTEEEIEYAIHHIAATVMKLNAMIYLL

Sequences:

>Translated_389_residues
MKLPIYFDYAATTPVDPRVVEKMIPYFTLDGIFGNPSSRSHYYGWKAEEAVDIARKQIAQLIGAESREIIFTSGATESVN
LAIKGIAQSYHKKGKHIVTSMTEHKSVLDTCQYLENKGFEITRITPQPNGLITFEQISNALRDDTILLSIMHVNNEIGVI
QDIKKFGKLCRLHDIIFHVDATQSVGKLHIDLSDLPVDLMSFNGHKLYGPKGIGGLYIRQHRHPKLHITAQMHGGGHEHG
MRSGTLPVHQIVGMAEAYRLARETMTTEMIYLKKMRDRLWQGLQQIEGILINGDLEYSIGTLLNISFNNIDGETLIIALK
DFALSSGSACTSGTLNPSHVLQALGRNNELAYNSIRFSLGRFTTEEEIEYAIHHIAATVMKLNAMIYLL
>Mature_389_residues
MKLPIYFDYAATTPVDPRVVEKMIPYFTLDGIFGNPSSRSHYYGWKAEEAVDIARKQIAQLIGAESREIIFTSGATESVN
LAIKGIAQSYHKKGKHIVTSMTEHKSVLDTCQYLENKGFEITRITPQPNGLITFEQISNALRDDTILLSIMHVNNEIGVI
QDIKKFGKLCRLHDIIFHVDATQSVGKLHIDLSDLPVDLMSFNGHKLYGPKGIGGLYIRQHRHPKLHITAQMHGGGHEHG
MRSGTLPVHQIVGMAEAYRLARETMTTEMIYLKKMRDRLWQGLQQIEGILINGDLEYSIGTLLNISFNNIDGETLIIALK
DFALSSGSACTSGTLNPSHVLQALGRNNELAYNSIRFSLGRFTTEEEIEYAIHHIAATVMKLNAMIYLL

Specific function: Catalyzes the removal of elemental sulfur and selenium atoms from cysteine and selenocysteine to produce alanine. Functions as a sulfur delivery protein for NAD, biotin and Fe-S cluster synthesis. Transfers sulfur on 'Cys-456' of ThiI in a transpersulfida

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=383, Percent_Identity=48.5639686684073, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI156713448, Length=415, Percent_Identity=30.1204819277108, Blast_Score=172, Evalue=3e-43,
Organism=Escherichia coli, GI48994898, Length=385, Percent_Identity=67.012987012987, Blast_Score=555, Evalue=1e-159,
Organism=Escherichia coli, GI1787970, Length=221, Percent_Identity=29.8642533936652, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789175, Length=215, Percent_Identity=25.5813953488372, Blast_Score=85, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI25143064, Length=371, Percent_Identity=50.6738544474393, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17533177, Length=323, Percent_Identity=31.8885448916409, Blast_Score=128, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6319831, Length=382, Percent_Identity=46.3350785340314, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI20129463, Length=383, Percent_Identity=48.0417754569191, Blast_Score=389, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 43532; Mature: 43532

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPIYFDYAATTPVDPRVVEKMIPYFTLDGIFGNPSSRSHYYGWKAEEAVDIARKQIAQ
CCCEEEEECCCCCCCCHHHHHHHCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LIGAESREIIFTSGATESVNLAIKGIAQSYHKKGKHIVTSMTEHKSVLDTCQYLENKGFE
HHCCCCCEEEEECCCCCEEEHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHCCCCE
ITRITPQPNGLITFEQISNALRDDTILLSIMHVNNEIGVIQDIKKFGKLCRLHDIIFHVD
EEEECCCCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHEEEEE
ATQSVGKLHIDLSDLPVDLMSFNGHKLYGPKGIGGLYIRQHRHPKLHITAQMHGGGHEHG
CCCCCCEEEEEHHHCCCHHHCCCCCEEECCCCCCCEEEEECCCCEEEEEEEECCCCCCCC
MRSGTLPVHQIVGMAEAYRLARETMTTEMIYLKKMRDRLWQGLQQIEGILINGDLEYSIG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEECE
TLLNISFNNIDGETLIIALKDFALSSGSACTSGTLNPSHVLQALGRNNELAYNSIRFSLG
EEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEHHHEEHHC
RFTTEEEIEYAIHHIAATVMKLNAMIYLL
CCCCHHHHHHHHHHHHHHHHHHHEEEEEC
>Mature Secondary Structure
MKLPIYFDYAATTPVDPRVVEKMIPYFTLDGIFGNPSSRSHYYGWKAEEAVDIARKQIAQ
CCCEEEEECCCCCCCCHHHHHHHCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LIGAESREIIFTSGATESVNLAIKGIAQSYHKKGKHIVTSMTEHKSVLDTCQYLENKGFE
HHCCCCCEEEEECCCCCEEEHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHCCCCE
ITRITPQPNGLITFEQISNALRDDTILLSIMHVNNEIGVIQDIKKFGKLCRLHDIIFHVD
EEEECCCCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHEEEEE
ATQSVGKLHIDLSDLPVDLMSFNGHKLYGPKGIGGLYIRQHRHPKLHITAQMHGGGHEHG
CCCCCCEEEEEHHHCCCHHHCCCCCEEECCCCCCCEEEEECCCCEEEEEEEECCCCCCCC
MRSGTLPVHQIVGMAEAYRLARETMTTEMIYLKKMRDRLWQGLQQIEGILINGDLEYSIG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEECE
TLLNISFNNIDGETLIIALKDFALSSGSACTSGTLNPSHVLQALGRNNELAYNSIRFSLG
EEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEHHHEEHHC
RFTTEEEIEYAIHHIAATVMKLNAMIYLL
CCCCHHHHHHHHHHHHHHHHHHHEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA