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The map label for this gene is cysP [H]

Identifier: 71892284

GI number: 71892284

Start: 634554

End: 635576

Strand: Reverse

Name: cysP [H]

Synonym: BPEN_531

Alternate gene names: 71892284

Gene position: 635576-634554 (Counterclockwise)

Preceding gene: 71892288

Following gene: 71892283

Centisome position: 80.28

GC content: 32.45

Gene sequence:

>1023_bases
ATGATTATTATGCATACTGTTTTAAAAATAATTAAAGTGACAGTATTATTGTACTGTGTTTTTTGTGTTCCAATACATGG
AACTATTTTATTAAATAGTTCTTATGATGTGTCTAGAGAATTATTTTTAAAGATTAATTCTGATTTTATCGAATATTGGA
AAGATCTTCATCCTGAAGATGAATTAATTATTCGTCAATCTCATGCAGGATCTACCAGACAAGCTATGGCTATATTACAG
GGACTTCGCGCGGATGTGGTGACATATAATCAGGTGATAGATGTACAAATTTTGTGTGATCGCGGACGCTTAATTCCTAA
AAATTGGCAAGATCGATTTCCACATCATAGTTCGCCATTTTTTTCAACTATGGCTTTTTTGGTGAGACGAGGCAATCCAA
AAGGAATTTATAATTGGTATGATTTAACGAATGAAGGGTTGAAAATAATATTTCCTAATCCTAAAACATCTGGTAATGGA
CGTTATACTTATTTATCGGCTTGGAATTGTTTTTTAAAAAATAGCGACGGAGATATAGAGCAAACTCGAATTTGGATGCA
AAAATTTTTAAAAAATGTAAAGGTATTTGATACTGGAGGACGCGCTGCTACCTCTACTTTTGTTGATCGTAATCAAGGAG
ATGTACTAATTAATTTTGAATCTGAAGCAAAATTAGTTTTAAGTCAATATGGATTTAATAATTATGAAATTGTTATACCC
ACTCCGAATGTTTTGGTAGAATTTCCAGTGACTTGGATAGATAAAAACGTCATTAGAAATGGAACCAAAGATGTAGCACG
AGCTTATTTAGATTACCTATACCTTCCTTCAGCACAAAAGATTATTACTAAGTTTGGATACCGCGTCAATACAACTGACA
TTGCTCAAATATATCCGAATGGATCTTCTGGGAGTCAATTATTTAGAATAGAGGATCAATTTGGTAATTGGAATACTGTT
ATGAATACCCACTTTAAACGTGGCGGTGAATTAGATCAATTGTTATCAATAGGACATCGTTGA

Upstream 100 bases:

>100_bases
GTGCAGTTTATGTCTTAATGATGCATGTATTATATAATGTGTTCTATCAACTGGAAGGAAAATCTCTATTTTGAGTAGGT
ATGTTTGTATTCGGATATAT

Downstream 100 bases:

>100_bases
TGTTATTTTCTTCATCTAAACATACCTTACCTGGGTTTGGTATAGCACTGGGCGGAAGTTTATTGTTTGTTTGTTTGATT
CTATTATTACCTTTAAGTGC

Product: thiosulfate ABC transporter periplasmic substrate-binding protein

Products: SO42- [Cytoplasm]; phosphate; ADP; S2O32- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MIIMHTVLKIIKVTVLLYCVFCVPIHGTILLNSSYDVSRELFLKINSDFIEYWKDLHPEDELIIRQSHAGSTRQAMAILQ
GLRADVVTYNQVIDVQILCDRGRLIPKNWQDRFPHHSSPFFSTMAFLVRRGNPKGIYNWYDLTNEGLKIIFPNPKTSGNG
RYTYLSAWNCFLKNSDGDIEQTRIWMQKFLKNVKVFDTGGRAATSTFVDRNQGDVLINFESEAKLVLSQYGFNNYEIVIP
TPNVLVEFPVTWIDKNVIRNGTKDVARAYLDYLYLPSAQKIITKFGYRVNTTDIAQIYPNGSSGSQLFRIEDQFGNWNTV
MNTHFKRGGELDQLLSIGHR

Sequences:

>Translated_340_residues
MIIMHTVLKIIKVTVLLYCVFCVPIHGTILLNSSYDVSRELFLKINSDFIEYWKDLHPEDELIIRQSHAGSTRQAMAILQ
GLRADVVTYNQVIDVQILCDRGRLIPKNWQDRFPHHSSPFFSTMAFLVRRGNPKGIYNWYDLTNEGLKIIFPNPKTSGNG
RYTYLSAWNCFLKNSDGDIEQTRIWMQKFLKNVKVFDTGGRAATSTFVDRNQGDVLINFESEAKLVLSQYGFNNYEIVIP
TPNVLVEFPVTWIDKNVIRNGTKDVARAYLDYLYLPSAQKIITKFGYRVNTTDIAQIYPNGSSGSQLFRIEDQFGNWNTV
MNTHFKRGGELDQLLSIGHR
>Mature_340_residues
MIIMHTVLKIIKVTVLLYCVFCVPIHGTILLNSSYDVSRELFLKINSDFIEYWKDLHPEDELIIRQSHAGSTRQAMAILQ
GLRADVVTYNQVIDVQILCDRGRLIPKNWQDRFPHHSSPFFSTMAFLVRRGNPKGIYNWYDLTNEGLKIIFPNPKTSGNG
RYTYLSAWNCFLKNSDGDIEQTRIWMQKFLKNVKVFDTGGRAATSTFVDRNQGDVLINFESEAKLVLSQYGFNNYEIVIP
TPNVLVEFPVTWIDKNVIRNGTKDVARAYLDYLYLPSAQKIITKFGYRVNTTDIAQIYPNGSSGSQLFRIEDQFGNWNTV
MNTHFKRGGELDQLLSIGHR

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. This protein specifically binds thiosulfate and is involved in its transmembrane transport [H]

COG id: COG4150

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1788765, Length=315, Percent_Identity=57.7777777777778, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1790351, Length=320, Percent_Identity=42.5, Blast_Score=273, Evalue=1e-74,

Paralogues:

None

Copy number: 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39124; Mature: 39124

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIMHTVLKIIKVTVLLYCVFCVPIHGTILLNSSYDVSRELFLKINSDFIEYWKDLHPED
CCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECHHHHHHHHHCCCCC
ELIIRQSHAGSTRQAMAILQGLRADVVTYNQVIDVQILCDRGRLIPKNWQDRFPHHSSPF
CEEEEECCCCCHHHHHHHHHHHHHHHEECCEEEEEEEEECCCCCCCCCHHHCCCCCCCHH
FSTMAFLVRRGNPKGIYNWYDLTNEGLKIIFPNPKTSGNGRYTYLSAWNCFLKNSDGDIE
HHHHHHHHHCCCCCCEEEHEECCCCCEEEEECCCCCCCCCCEEEEEEEEEEEECCCCCHH
QTRIWMQKFLKNVKVFDTGGRAATSTFVDRNQGDVLINFESEAKLVLSQYGFNNYEIVIP
HHHHHHHHHHCCCEEEECCCCEEHHHHEECCCCCEEEEECCCHHHHHHHCCCCCEEEEEE
TPNVLVEFPVTWIDKNVIRNGTKDVARAYLDYLYLPSAQKIITKFGYRVNTTDIAQIYPN
CCCEEEECCHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHCCCEECCCEEEEEECC
GSSGSQLFRIEDQFGNWNTVMNTHFKRGGELDQLLSIGHR
CCCCCEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHCCCC
>Mature Secondary Structure
MIIMHTVLKIIKVTVLLYCVFCVPIHGTILLNSSYDVSRELFLKINSDFIEYWKDLHPED
CCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECHHHHHHHHHCCCCC
ELIIRQSHAGSTRQAMAILQGLRADVVTYNQVIDVQILCDRGRLIPKNWQDRFPHHSSPF
CEEEEECCCCCHHHHHHHHHHHHHHHEECCEEEEEEEEECCCCCCCCCHHHCCCCCCCHH
FSTMAFLVRRGNPKGIYNWYDLTNEGLKIIFPNPKTSGNGRYTYLSAWNCFLKNSDGDIE
HHHHHHHHHCCCCCCEEEHEECCCCCEEEEECCCCCCCCCCEEEEEEEEEEEECCCCCHH
QTRIWMQKFLKNVKVFDTGGRAATSTFVDRNQGDVLINFESEAKLVLSQYGFNNYEIVIP
HHHHHHHHHHCCCEEEECCCCEEHHHHEECCCCCEEEEECCCHHHHHHHCCCCCEEEEEE
TPNVLVEFPVTWIDKNVIRNGTKDVARAYLDYLYLPSAQKIITKFGYRVNTTDIAQIYPN
CCCEEEECCHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHCCCEECCCEEEEEECC
GSSGSQLFRIEDQFGNWNTVMNTHFKRGGELDQLLSIGHR
CCCCCEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: SO42- [Periplasm]; H2O; ATP; S2O32- [Periplasm] [C]

Specific reaction: SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2188959; 9205837; 9278503; 9298646 [H]