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The map label for this gene is cysU [H]

Identifier: 71892283

GI number: 71892283

Start: 633721

End: 634554

Strand: Reverse

Name: cysU [H]

Synonym: BPEN_530

Alternate gene names: 71892283

Gene position: 634554-633721 (Counterclockwise)

Preceding gene: 71892284

Following gene: 71892282

Centisome position: 80.16

GC content: 34.89

Gene sequence:

>834_bases
ATGTTATTTTCTTCATCTAAACATACCTTACCTGGGTTTGGTATAGCACTGGGCGGAAGTTTATTGTTTGTTTGTTTGAT
TCTATTATTACCTTTAAGTGCGTTAGTTATGCAATTATCTCAGATGAGTCTTTCTCAATATTGGGATGTAATTACAGATC
CAGCATTATTGGTTTCTTATCAAATCACCTTATTAGCTGCTGGGACAGCAACATTATTTAATGCTATATTTGGACTGTTA
GTATCATGGGTAATAACTAGATATCAATTTCCTGGTCGGAAATTATTAGATGCGTTAGTAGATTTACCTTTTGCTTTGCC
TACTGCGGTGGCGGGATTAACTTTGGCGACATTGTTTTCAACGTCAGGTTGGTATGGGTCTTGGTTATCTCGATTCGGCA
TCACAGTATCGTATACGTGGATAGGTATATCTATTGCTATGATTTTTACAAGTATTCCATTTGTAGTACGAACCGTACAA
CCGGTGCTAGAAGAGTTTACTGTAGAATACGAAGAAGTAGCAGAAACTCTTGGAGCTGATCATTGGCAAATTTTTTGTAA
TGTAATTTTTCCAGAATTGGCACCAGCTTGGTTGTATGGCGTAGCGTTGTCGTTTATACGAAGTTTAGGTGAATTTGGTG
CGGTTATTTTTATAGCTGGAAATGTTGCCTGGAAAAATGAAGTTATTTCTTTAATTATCTTTATTCGTTTACAGGAATTT
GATTATCCAGCTGCCAGTGCTATTTCTTCTGTAATTTTAATTATTTCTTTATTATTATTATTTTTTACTAGTATGTTTCA
ATTACGAATAAACCGGAGGTTTAAGGGATCTTAA

Upstream 100 bases:

>100_bases
TTAGAATAGAGGATCAATTTGGTAATTGGAATACTGTTATGAATACCCACTTTAAACGTGGCGGTGAATTAGATCAATTG
TTATCAATAGGACATCGTTG

Downstream 100 bases:

>100_bases
TGATCAGCATGTTGAATAATTATGTCAATAAAAATTGCAATAATGATTGCAACAGTAGTATTAAATGGAACCAATGGATT
CTAATTGGTTTCACTATGTT

Product: sulfate/thiosulfate transporter subunit

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MLFSSSKHTLPGFGIALGGSLLFVCLILLLPLSALVMQLSQMSLSQYWDVITDPALLVSYQITLLAAGTATLFNAIFGLL
VSWVITRYQFPGRKLLDALVDLPFALPTAVAGLTLATLFSTSGWYGSWLSRFGITVSYTWIGISIAMIFTSIPFVVRTVQ
PVLEEFTVEYEEVAETLGADHWQIFCNVIFPELAPAWLYGVALSFIRSLGEFGAVIFIAGNVAWKNEVISLIIFIRLQEF
DYPAASAISSVILIISLLLLFFTSMFQLRINRRFKGS

Sequences:

>Translated_277_residues
MLFSSSKHTLPGFGIALGGSLLFVCLILLLPLSALVMQLSQMSLSQYWDVITDPALLVSYQITLLAAGTATLFNAIFGLL
VSWVITRYQFPGRKLLDALVDLPFALPTAVAGLTLATLFSTSGWYGSWLSRFGITVSYTWIGISIAMIFTSIPFVVRTVQ
PVLEEFTVEYEEVAETLGADHWQIFCNVIFPELAPAWLYGVALSFIRSLGEFGAVIFIAGNVAWKNEVISLIIFIRLQEF
DYPAASAISSVILIISLLLLFFTSMFQLRINRRFKGS
>Mature_277_residues
MLFSSSKHTLPGFGIALGGSLLFVCLILLLPLSALVMQLSQMSLSQYWDVITDPALLVSYQITLLAAGTATLFNAIFGLL
VSWVITRYQFPGRKLLDALVDLPFALPTAVAGLTLATLFSTSGWYGSWLSRFGITVSYTWIGISIAMIFTSIPFVVRTVQ
PVLEEFTVEYEEVAETLGADHWQIFCNVIFPELAPAWLYGVALSFIRSLGEFGAVIFIAGNVAWKNEVISLIIFIRLQEF
DYPAASAISSVILIISLLLLFFTSMFQLRINRRFKGS

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=276, Percent_Identity=69.9275362318841, Blast_Score=352, Evalue=2e-98,
Organism=Escherichia coli, GI87082099, Length=261, Percent_Identity=34.8659003831418, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1786980, Length=231, Percent_Identity=33.3333333333333, Blast_Score=107, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30633; Mature: 30633

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFSSSKHTLPGFGIALGGSLLFVCLILLLPLSALVMQLSQMSLSQYWDVITDPALLVSY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
QITLLAAGTATLFNAIFGLLVSWVITRYQFPGRKLLDALVDLPFALPTAVAGLTLATLFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
TSGWYGSWLSRFGITVSYTWIGISIAMIFTSIPFVVRTVQPVLEEFTVEYEEVAETLGAD
CCCCHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCH
HWQIFCNVIFPELAPAWLYGVALSFIRSLGEFGAVIFIAGNVAWKNEVISLIIFIRLQEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHC
DYPAASAISSVILIISLLLLFFTSMFQLRINRRFKGS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLFSSSKHTLPGFGIALGGSLLFVCLILLLPLSALVMQLSQMSLSQYWDVITDPALLVSY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
QITLLAAGTATLFNAIFGLLVSWVITRYQFPGRKLLDALVDLPFALPTAVAGLTLATLFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
TSGWYGSWLSRFGITVSYTWIGISIAMIFTSIPFVVRTVQPVLEEFTVEYEEVAETLGAD
CCCCHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCH
HWQIFCNVIFPELAPAWLYGVALSFIRSLGEFGAVIFIAGNVAWKNEVISLIIFIRLQEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHC
DYPAASAISSVILIISLLLLFFTSMFQLRINRRFKGS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]