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The map label for this gene is cysW [H]

Identifier: 71892282

GI number: 71892282

Start: 632861

End: 633721

Strand: Reverse

Name: cysW [H]

Synonym: BPEN_529

Alternate gene names: 71892282

Gene position: 633721-632861 (Counterclockwise)

Preceding gene: 71892283

Following gene: 71892281

Centisome position: 80.05

GC content: 30.89

Gene sequence:

>861_bases
ATGATCAGCATGTTGAATAATTATGTCAATAAAAATTGCAATAATGATTGCAACAGTAGTATTAAATGGAACCAATGGAT
TCTAATTGGTTTCACTATGTTTATATCAATGATGTTGTTGTTAGTTCCTTTAATTGTGATTTTTGTATCTGCTTTTTCAG
AAGGTCTAAAAATAGTAATAGTAAATTTAATAAATCAAGATATGATACATGCTACTTTTTTGACGGTTATTATAGCGTTA
TTTACGGTACCTATTAATGTATTTTTTGGTGTATTAATGGCTTGGTTGGTGACTCGTTTTAAATTTTATGGAAGACAGTT
ATTGTATATATTAGTAAATATACCCATCGCTGTTTCTCCGGTAATTGTTGGATTATTGTATTTATTGCTTTATGCAAATA
ATAATGCAATAGCAAGTTGGCTGGATTTGCATAATATACAAATAATATTCACATGGGTAGGATTAGTATTAGTTACTATT
TTTGTTACTTGTCCTTTTGTGGTACATGAGCTTGTTCCTATGATGGTGAATCAGGGAAGACAAGAAGACGAGGCAGCGAT
ACTTCTTGGAGCATCTGGTTGGATGATGTTTCGTTATGTTACTTTTCCAAATATTCGTTGGGCTTTGTTATACGGGGCTA
TTCTTACTAATTCTCGTGCTATTGGTGAATTTGGAGCAGTATCTATAGTATCAGGGTTAATACGGGGTGAAACATATACT
GTTTCGTTGTATGTAGAATTATTATATCAAGATTACAATACTGTAGGCGCATTTGTTGCGGCATCATTATTAGCTTGTAT
TTCAATTCTTATGTTGTTTATTAAACATTATTTACAAAGTCGTTTAAAACGTAATAATTAA

Upstream 100 bases:

>100_bases
CCAGTGCTATTTCTTCTGTAATTTTAATTATTTCTTTATTATTATTATTTTTTACTAGTATGTTTCAATTACGAATAAAC
CGGAGGTTTAAGGGATCTTA

Downstream 100 bases:

>100_bases
TATTATGATCAAGGATAAGAAATGAGCATTGAGATAGATGGAATCACGAAATTTTTTGGTCATGATAAAATTTTGACTAA
TGTTTCTTTACATATTGAAT

Product: thiosulfate permease W

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MISMLNNYVNKNCNNDCNSSIKWNQWILIGFTMFISMMLLLVPLIVIFVSAFSEGLKIVIVNLINQDMIHATFLTVIIAL
FTVPINVFFGVLMAWLVTRFKFYGRQLLYILVNIPIAVSPVIVGLLYLLLYANNNAIASWLDLHNIQIIFTWVGLVLVTI
FVTCPFVVHELVPMMVNQGRQEDEAAILLGASGWMMFRYVTFPNIRWALLYGAILTNSRAIGEFGAVSIVSGLIRGETYT
VSLYVELLYQDYNTVGAFVAASLLACISILMLFIKHYLQSRLKRNN

Sequences:

>Translated_286_residues
MISMLNNYVNKNCNNDCNSSIKWNQWILIGFTMFISMMLLLVPLIVIFVSAFSEGLKIVIVNLINQDMIHATFLTVIIAL
FTVPINVFFGVLMAWLVTRFKFYGRQLLYILVNIPIAVSPVIVGLLYLLLYANNNAIASWLDLHNIQIIFTWVGLVLVTI
FVTCPFVVHELVPMMVNQGRQEDEAAILLGASGWMMFRYVTFPNIRWALLYGAILTNSRAIGEFGAVSIVSGLIRGETYT
VSLYVELLYQDYNTVGAFVAASLLACISILMLFIKHYLQSRLKRNN
>Mature_286_residues
MISMLNNYVNKNCNNDCNSSIKWNQWILIGFTMFISMMLLLVPLIVIFVSAFSEGLKIVIVNLINQDMIHATFLTVIIAL
FTVPINVFFGVLMAWLVTRFKFYGRQLLYILVNIPIAVSPVIVGLLYLLLYANNNAIASWLDLHNIQIIFTWVGLVLVTI
FVTCPFVVHELVPMMVNQGRQEDEAAILLGASGWMMFRYVTFPNIRWALLYGAILTNSRAIGEFGAVSIVSGLIRGETYT
VSLYVELLYQDYNTVGAFVAASLLACISILMLFIKHYLQSRLKRNN

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=267, Percent_Identity=64.4194756554307, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1788764, Length=223, Percent_Identity=34.5291479820628, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1786980, Length=212, Percent_Identity=31.1320754716981, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32369; Mature: 32369

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISMLNNYVNKNCNNDCNSSIKWNQWILIGFTMFISMMLLLVPLIVIFVSAFSEGLKIVI
CHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VNLINQDMIHATFLTVIIALFTVPINVFFGVLMAWLVTRFKFYGRQLLYILVNIPIAVSP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VIVGLLYLLLYANNNAIASWLDLHNIQIIFTWVGLVLVTIFVTCPFVVHELVPMMVNQGR
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QEDEAAILLGASGWMMFRYVTFPNIRWALLYGAILTNSRAIGEFGAVSIVSGLIRGETYT
CCCCCEEEEECCCCEEEEEEECCCHHHHHHHHHHHHCCCHHCHHHHHHHHHHHHCCCEEE
VSLYVELLYQDYNTVGAFVAASLLACISILMLFIKHYLQSRLKRNN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MISMLNNYVNKNCNNDCNSSIKWNQWILIGFTMFISMMLLLVPLIVIFVSAFSEGLKIVI
CHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VNLINQDMIHATFLTVIIALFTVPINVFFGVLMAWLVTRFKFYGRQLLYILVNIPIAVSP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VIVGLLYLLLYANNNAIASWLDLHNIQIIFTWVGLVLVTIFVTCPFVVHELVPMMVNQGR
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QEDEAAILLGASGWMMFRYVTFPNIRWALLYGAILTNSRAIGEFGAVSIVSGLIRGETYT
CCCCCEEEEECCCCEEEEEEECCCHHHHHHHHHHHHCCCHHCHHHHHHHHHHHHCCCEEE
VSLYVELLYQDYNTVGAFVAASLLACISILMLFIKHYLQSRLKRNN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]