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The map label for this gene is nupC [H]

Identifier: 71892275

GI number: 71892275

Start: 622114

End: 623304

Strand: Direct

Name: nupC [H]

Synonym: BPEN_519

Alternate gene names: 71892275

Gene position: 622114-623304 (Clockwise)

Preceding gene: 71892273

Following gene: 71892278

Centisome position: 78.58

GC content: 29.05

Gene sequence:

>1191_bases
GTGTCTCGCATTTTTCATTTTGTCTTAGGATTTACTACAATAGTAATTCTGTCACTGATTGTCAGTAGTAATCCAAAAAA
AATTCGTGTACGCTTTATTTTTCAAGTATTAATAATAGAAATTTTATTTGGATATTTTATTTTACATTCCCAATTAGGAT
CAAATTCCATACGAAAATTATGTACTTTGTTCGATACACTATTAATTTTTGCATCAGAAGGAACAAACTTTATCTTTGGA
GGAATGCACGCACAGGGATTAGCTACTTTTTTCTTAAACATACTTTGTCCAATAATTTTTATTTCCGCGCTAATTGGTAT
TTTAAAATATACTCATATATTAGTATTTTCTATTCGAATAATTGGAACTATTTTATCCAAAATTAATGGCATGGGAAAGT
TAGAATCTTTTAATGCTGTTAGTTCATTGATCTTAGGGCAATCTGAAAATTTTATAGTATATAAAAATATTATAGGAAAA
ATACCTGCACCACGAATGTATACCATGGCGGCAACTGCCATGTCTACAGTGTCTCTTTCCATAATTGGTGCTTATATGAC
CATGCTAGCTCCAAAATATGTTGTAGCGGCATTAATATTAAATATGTTTAGTGCTTTTATCATATTATCATTACTGAATC
CTTATGTAGTAGATAAAGAAAAAGATATAGACATCACCGATTTTTACATCAATAATCAAAATATTTTTGAAATTATAGGA
GAATATATTTTAATTGGATTCAAAATAGCTGTTACTGTTGCTGCAATGTTGATAGGGTTTATCGCGCTCATTTCCGCTCT
TAATTTCACATTTAAAATATTATTTAATACATCTTTACAAGAAATTTTAGGATATATTTTTTTTCCTTGCGCTTGGATTA
TGGGTATACCTACTGATGAAATACTACCAGTCAGCAGTATTATGGCAACTAAATTAATTTCTAATGAATTTATTGCAATT
ATAAACCTACAAAACATTTCTAGTGATTTATCGCCTCAAACTGTAGGAATAACATCAGTTTTCTTGGTTTCATTTTCTAA
TTTCTCATCTATTGGTATTATTGCAGGTGCTATTAAAGGACTTAATAAAACACAAGGTAATATGATTTCACAACATCTCG
GATTTAAACTAATATATAGCTCCACGCTCATCAATATGCTATCTGCTTCAATAGTTGGTTTACTTATATGA

Upstream 100 bases:

>100_bases
TTTTTAATGAATTACAAGATGTACAAATAAATTTATTGAATTTCTGTTTTATATAGAATAATACAAATAATTTCTGCGCA
ATATTGCTGGAGTAAAGATT

Downstream 100 bases:

>100_bases
TGATTATTGCTAACTATTAAATGATAAATCAAGATATTTAATATTATTTAGAGATAAAAACTTCTAATAATCTAGATTGC
ATATAGCAAATAATTTTTTA

Product: NupC family nucleoside transporter

Products: uridine [Cytoplasm]; thymidine [Cytoplasm]; inosine [Cytoplasm]; Deoxyuridine [Cytoplasm]; deoxyinosine [Cytoplasm]; deoxycytidine [Cytoplasm]; deoxyadenosine [Cytoplasm]; cytidine [Cytoplasm]; Proton [Cytoplasm]; adenosine [Cytoplasm] [C]

Alternate protein names: Nucleoside-transport system protein nupC [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSRIFHFVLGFTTIVILSLIVSSNPKKIRVRFIFQVLIIEILFGYFILHSQLGSNSIRKLCTLFDTLLIFASEGTNFIFG
GMHAQGLATFFLNILCPIIFISALIGILKYTHILVFSIRIIGTILSKINGMGKLESFNAVSSLILGQSENFIVYKNIIGK
IPAPRMYTMAATAMSTVSLSIIGAYMTMLAPKYVVAALILNMFSAFIILSLLNPYVVDKEKDIDITDFYINNQNIFEIIG
EYILIGFKIAVTVAAMLIGFIALISALNFTFKILFNTSLQEILGYIFFPCAWIMGIPTDEILPVSSIMATKLISNEFIAI
INLQNISSDLSPQTVGITSVFLVSFSNFSSIGIIAGAIKGLNKTQGNMISQHLGFKLIYSSTLINMLSASIVGLLI

Sequences:

>Translated_396_residues
MSRIFHFVLGFTTIVILSLIVSSNPKKIRVRFIFQVLIIEILFGYFILHSQLGSNSIRKLCTLFDTLLIFASEGTNFIFG
GMHAQGLATFFLNILCPIIFISALIGILKYTHILVFSIRIIGTILSKINGMGKLESFNAVSSLILGQSENFIVYKNIIGK
IPAPRMYTMAATAMSTVSLSIIGAYMTMLAPKYVVAALILNMFSAFIILSLLNPYVVDKEKDIDITDFYINNQNIFEIIG
EYILIGFKIAVTVAAMLIGFIALISALNFTFKILFNTSLQEILGYIFFPCAWIMGIPTDEILPVSSIMATKLISNEFIAI
INLQNISSDLSPQTVGITSVFLVSFSNFSSIGIIAGAIKGLNKTQGNMISQHLGFKLIYSSTLINMLSASIVGLLI
>Mature_395_residues
SRIFHFVLGFTTIVILSLIVSSNPKKIRVRFIFQVLIIEILFGYFILHSQLGSNSIRKLCTLFDTLLIFASEGTNFIFGG
MHAQGLATFFLNILCPIIFISALIGILKYTHILVFSIRIIGTILSKINGMGKLESFNAVSSLILGQSENFIVYKNIIGKI
PAPRMYTMAATAMSTVSLSIIGAYMTMLAPKYVVAALILNMFSAFIILSLLNPYVVDKEKDIDITDFYINNQNIFEIIGE
YILIGFKIAVTVAAMLIGFIALISALNFTFKILFNTSLQEILGYIFFPCAWIMGIPTDEILPVSSIMATKLISNEFIAII
NLQNISSDLSPQTVGITSVFLVSFSNFSSIGIIAGAIKGLNKTQGNMISQHLGFKLIYSSTLINMLSASIVGLLI

Specific function: Transports nucleosides with a high affinity except guanosine and deoxyguanosine. Driven by a proton motive force [H]

COG id: COG1972

COG function: function code F; Nucleoside permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family [H]

Homologues:

Organism=Homo sapiens, GI42542381, Length=412, Percent_Identity=21.6019417475728, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI11545853, Length=360, Percent_Identity=22.5, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI227116277, Length=412, Percent_Identity=19.6601941747573, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788737, Length=402, Percent_Identity=62.1890547263682, Blast_Score=500, Evalue=1e-143,
Organism=Escherichia coli, GI1788485, Length=412, Percent_Identity=28.3980582524272, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI1788488, Length=412, Percent_Identity=27.6699029126214, Blast_Score=152, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17560276, Length=406, Percent_Identity=25.3694581280788, Blast_Score=104, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI71991794, Length=393, Percent_Identity=26.4631043256997, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI45552517, Length=392, Percent_Identity=23.7244897959184, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI19921868, Length=392, Percent_Identity=23.7244897959184, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI45552519, Length=392, Percent_Identity=23.7244897959184, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI281360430, Length=340, Percent_Identity=22.9411764705882, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008276
- InterPro:   IPR018270
- InterPro:   IPR011657
- InterPro:   IPR002668 [H]

Pfam domain/function: PF07662 Nucleos_tra2_C; PF01773 Nucleos_tra2_N [H]

EC number: NA

Molecular weight: Translated: 43567; Mature: 43436

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIFHFVLGFTTIVILSLIVSSNPKKIRVRFIFQVLIIEILFGYFILHSQLGSNSIRKL
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
CTLFDTLLIFASEGTNFIFGGMHAQGLATFFLNILCPIIFISALIGILKYTHILVFSIRI
HHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGTILSKINGMGKLESFNAVSSLILGQSENFIVYKNIIGKIPAPRMYTMAATAMSTVSLS
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEHHHHCCCCCCCHHHHHHHHHHHHHHH
IIGAYMTMLAPKYVVAALILNMFSAFIILSLLNPYVVDKEKDIDITDFYINNQNIFEIIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCEEEEEECCCHHHHHHH
EYILIGFKIAVTVAAMLIGFIALISALNFTFKILFNTSLQEILGYIFFPCAWIMGIPTDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHCCCCCC
ILPVSSIMATKLISNEFIAIINLQNISSDLSPQTVGITSVFLVSFSNFSSIGIIAGAIKG
CCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHC
LNKTQGNMISQHLGFKLIYSSTLINMLSASIVGLLI
CCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRIFHFVLGFTTIVILSLIVSSNPKKIRVRFIFQVLIIEILFGYFILHSQLGSNSIRKL
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
CTLFDTLLIFASEGTNFIFGGMHAQGLATFFLNILCPIIFISALIGILKYTHILVFSIRI
HHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGTILSKINGMGKLESFNAVSSLILGQSENFIVYKNIIGKIPAPRMYTMAATAMSTVSLS
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEHHHHCCCCCCCHHHHHHHHHHHHHHH
IIGAYMTMLAPKYVVAALILNMFSAFIILSLLNPYVVDKEKDIDITDFYINNQNIFEIIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCEEEEEECCCHHHHHHH
EYILIGFKIAVTVAAMLIGFIALISALNFTFKILFNTSLQEILGYIFFPCAWIMGIPTDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHCCCCCC
ILPVSSIMATKLISNEFIAIINLQNISSDLSPQTVGITSVFLVSFSNFSSIGIIAGAIKG
CCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHC
LNKTQGNMISQHLGFKLIYSSTLINMLSASIVGLLI
CCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine [Periplasm]; thymidine [Periplasm]; inosine [Periplasm]; Deoxyuridine [Periplasm]; deoxyinosine [Periplasm]; deoxycytidine [Periplasm]; deoxyadenosine [Periplasm]; cytidine [Periplasm]; Proton [Periplasm]; adenosine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uridine [Periplasm] = Proton [Cytoplasm] + uridine [Cytoplasm] Proton [Periplasm] + thymidine [Periplasm] = Proton [Cytoplasm] + thymidine [Cytoplasm] Proton [Periplasm] + inosine [Periplasm] = Proton [Cytoplasm] + inosine [Cytoplasm]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]