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The map label for this gene is hisF

Identifier: 71892241

GI number: 71892241

Start: 575413

End: 576189

Strand: Direct

Name: hisF

Synonym: BPEN_483

Alternate gene names: 71892241

Gene position: 575413-576189 (Clockwise)

Preceding gene: 71892240

Following gene: 71892242

Centisome position: 72.68

GC content: 32.95

Gene sequence:

>777_bases
ATGTTGGCAAAACGAATAATTCCTTGTCTTGATGTAGGTAATAACAAAGTAATTAAAGGAATACAATTTAAAGATCATAA
AATTGTAGGAGATATCTTAGAATTAGCAAATAGATATGCAAAATCAGGAGCCGATGAATTAGTATTTTATGACATCACAG
CATCACCTAATGATCAGGTAGTGAATAAGAGATGGATTTCTCAGATTGCTAAAGTAATAAACATACCATTCTGTGTGGCT
GGGGGCATAAATACATTAAAACAAGCTAAACAAATTCTTGCATCTGGAGCAGATAAAATTTCAATTAATTCTCCTGCTTT
AATTAACCCAATGCTAATCCAAGAACTAGCTGATCATTTAGGCACACAATGCGTTGTAGTTAGCATCGATACCTGGCATA
ATCCTCAAAAAAAGATTTACCAAGTAAAATGCTATACTGGGGACAGTACTCGTGCAAAAATCACAACATGGCAAACATTG
GATTGGGTTAAAAAGGTACAAGAATATGGAGCTGGAGAAATAGTATTAAATATGATGAATCAAGACGGAATGAAAAATGG
TTATGATTTAGATCAATTACGAAATATTAGAAAACATTGTCAAGTACCACTTATAGCTTCTGGAGGAGCTGGTACCTATC
AACATTTCTTAGAAGCCTTTCGTGATGCCGACGTAGATGGGGCGTTAGCAGCTTCAGTATTTCATAATCAAATTATTGAC
ATTCATAAATTGAAACAATTTTTAAATAAAGAAGGAATAAAAATTAGATTATGCTAA

Upstream 100 bases:

>100_bases
AATTATCAGAAATATCTAAATTACGTTCTTCTGGAGTGGCAGGAATCATTATTGGTCGTGCTTTTTTAGAAAATACATTT
ACTATCAATGAGGCTATATC

Downstream 100 bases:

>100_bases
TTAAAAATCAGCATCAGCAATTAAATTGGACTAAAAATCATGGTCTAATACCCGCTATCATACAACATGCTGAATCAGGA
GAAGTATTAATGCTAGGGCA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLAKRIIPCLDVGNNKVIKGIQFKDHKIVGDILELANRYAKSGADELVFYDITASPNDQVVNKRWISQIAKVINIPFCVA
GGINTLKQAKQILASGADKISINSPALINPMLIQELADHLGTQCVVVSIDTWHNPQKKIYQVKCYTGDSTRAKITTWQTL
DWVKKVQEYGAGEIVLNMMNQDGMKNGYDLDQLRNIRKHCQVPLIASGGAGTYQHFLEAFRDADVDGALAASVFHNQIID
IHKLKQFLNKEGIKIRLC

Sequences:

>Translated_258_residues
MLAKRIIPCLDVGNNKVIKGIQFKDHKIVGDILELANRYAKSGADELVFYDITASPNDQVVNKRWISQIAKVINIPFCVA
GGINTLKQAKQILASGADKISINSPALINPMLIQELADHLGTQCVVVSIDTWHNPQKKIYQVKCYTGDSTRAKITTWQTL
DWVKKVQEYGAGEIVLNMMNQDGMKNGYDLDQLRNIRKHCQVPLIASGGAGTYQHFLEAFRDADVDGALAASVFHNQIID
IHKLKQFLNKEGIKIRLC
>Mature_258_residues
MLAKRIIPCLDVGNNKVIKGIQFKDHKIVGDILELANRYAKSGADELVFYDITASPNDQVVNKRWISQIAKVINIPFCVA
GGINTLKQAKQILASGADKISINSPALINPMLIQELADHLGTQCVVVSIDTWHNPQKKIYQVKCYTGDSTRAKITTWQTL
DWVKKVQEYGAGEIVLNMMNQDGMKNGYDLDQLRNIRKHCQVPLIASGGAGTYQHFLEAFRDADVDGALAASVFHNQIID
IHKLKQFLNKEGIKIRLC

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=65.8914728682171, Blast_Score=376, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6319725, Length=317, Percent_Identity=28.7066246056782, Blast_Score=126, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_BLOPB (Q494D6)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277975.1
- ProteinModelPortal:   Q494D6
- SMR:   Q494D6
- STRING:   Q494D6
- GeneID:   3562990
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_483
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   ASVFHKG
- ProtClustDB:   PRK02083
- BioCyc:   CBLO291272:BPEN_483-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 28758; Mature: 28758

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVGNNKVIKGIQFKDHKIVGDILELANRYAKSGADELVFYDITASPNDQV
CCHHHCCHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHH
VNKRWISQIAKVINIPFCVAGGINTLKQAKQILASGADKISINSPALINPMLIQELADHL
HHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHC
GTQCVVVSIDTWHNPQKKIYQVKCYTGDSTRAKITTWQTLDWVKKVQEYGAGEIVLNMMN
CCEEEEEEEECCCCCCCEEEEEEEEECCCCCEEEEEHHHHHHHHHHHHCCCCCEEHEEHH
QDGMKNGYDLDQLRNIRKHCQVPLIASGGAGTYQHFLEAFRDADVDGALAASVFHNQIID
CCCCCCCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IHKLKQFLNKEGIKIRLC
HHHHHHHHCCCCCEEEEC
>Mature Secondary Structure
MLAKRIIPCLDVGNNKVIKGIQFKDHKIVGDILELANRYAKSGADELVFYDITASPNDQV
CCHHHCCHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHH
VNKRWISQIAKVINIPFCVAGGINTLKQAKQILASGADKISINSPALINPMLIQELADHL
HHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHC
GTQCVVVSIDTWHNPQKKIYQVKCYTGDSTRAKITTWQTLDWVKKVQEYGAGEIVLNMMN
CCEEEEEEEECCCCCCCEEEEEEEEECCCCCEEEEEHHHHHHHHHHHHCCCCCEEHEEHH
QDGMKNGYDLDQLRNIRKHCQVPLIASGGAGTYQHFLEAFRDADVDGALAASVFHNQIID
CCCCCCCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IHKLKQFLNKEGIKIRLC
HHHHHHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA