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The map label for this gene is cls [H]

Identifier: 71892208

GI number: 71892208

Start: 528045

End: 529514

Strand: Direct

Name: cls [H]

Synonym: BPEN_446

Alternate gene names: 71892208

Gene position: 528045-529514 (Clockwise)

Preceding gene: 71892206

Following gene: 71892210

Centisome position: 66.7

GC content: 34.49

Gene sequence:

>1470_bases
ATGATTGCGATTCATGCCATATTTAATTGTATTTTCTTATGCAGTTATTGGTTATTAATCGTACTAGTTACATTTCGAGT
TTTAATAAAACGCCGAGCAGTACCATCGTCTATGGCATGGTTATTAGTAATTTATGTTTTGCCTTTAATAGGTATCATTA
CATATCTATTATTTGGAGAATCTAACCTTGGTAAACAACGATCTAATAGAAGTAAGATAGCATGGTCATCTACTATGCGT
CGTATACGAATACTAAAAACCTACAAACATATATTTTCCACTGAACACAGCGTAATCGCTTCTTCATTGTTTAAGCTTTG
CGAACATCGTCAAGGAATAGGCGCTCTAAAAGGTAATCAAATACAGTTATTAAAAAAAACTGACGATACCATTATATCTT
TAATTAAAGATATTGAATTGGCTCAACACAGCATTGATATGGTATTTTATATTTGGGAGTCTGGGGGACTAGTAGATCAT
GTTATAAAAAAATTAATTATAGCAGCTAAACGAGGCATCCGATGTCGCATAATATTAGATTCAGCCGGATGCGCCAATTT
TTTCAGTAGTTCATGTCCTAATATAATGCGTCAAGCTGGGGTGAATATTGTAGAAGCACTACATGTCAATTTGTTACGCG
TCTTCTTACGTCGTATGGATTTGCGACAACACAGAAAAATGATATTAATTGATGACCATATTGCTTATACTGGCAGTATG
AATATGGTAGATCCACAATTGTTTAAAAAAAACATAGGAATTGGGCAATGGGTCGACATTATGGTGCGTATAGACGGGCC
TGCAGCTTCAGCAATGAGCATAATATTTTCTTCTGATTGGGAAATTGAAACAGGACAACACGTATTGTTCCCATTGTTAT
TCAACACTCACATAGTAGCAGCTCATAAACTAATATCAACAGGACATACTATTCAAATTATTCCCTCTGGACCAGGATGC
CCGGAGGGAATAATTCATCAAGCATTACTAATATCATTATATGAAGCACGTAAAAAGCTTATTATTACTACTCCATATTT
GGTGCCTAGTGATGATTTATTACATGCCATTTGTACAGCAGCACAACGGGGAGTAGAAGTACATATTATTATTCCTCAAA
ACAACGACTCTGTATTAGTAAACTGGGCTAGTAGAGCATTTTTTAGCGAATTATTAAAAGCCGGAGTTTTAATACATCAA
TTCCAAAATGGATTACTACACGTAAAAAGTGTATTAATCGATCAACAATTAAGTCTAATAGGAACCGTAAACCTGGACAT
ACGCAGTTTATGGCTAAACTTTGAAATCACATTAGTCATTGATAGTAAAGATTTTAGTAACGAGCTAGAAAAAACACAGG
AGAATTATATTGCACACTCTAAAACAATTGATCCCCAAAAATGGGCTAACCGTCCTTATTGGGAACGCATTGTTGAACGT
TTATTTTATTTCTTAAGTCCTCTGTTATAA

Upstream 100 bases:

>100_bases
TTATATGATTATAAAATTTGTCATAAAATAAATATTCAATAATAATCTATAGTCTTTGTTTAAAATATCTCATAAATATC
TCAATAGGATTTATGTAATC

Downstream 100 bases:

>100_bases
GGATTTTTTAACTTTTAATTTCAACAACATAATTCAAAATAAATATAACAAATCCCATACCACATCCATTAAATAGGGTG
TTATTAATTTGAATTAAAAT

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MIAIHAIFNCIFLCSYWLLIVLVTFRVLIKRRAVPSSMAWLLVIYVLPLIGIITYLLFGESNLGKQRSNRSKIAWSSTMR
RIRILKTYKHIFSTEHSVIASSLFKLCEHRQGIGALKGNQIQLLKKTDDTIISLIKDIELAQHSIDMVFYIWESGGLVDH
VIKKLIIAAKRGIRCRIILDSAGCANFFSSSCPNIMRQAGVNIVEALHVNLLRVFLRRMDLRQHRKMILIDDHIAYTGSM
NMVDPQLFKKNIGIGQWVDIMVRIDGPAASAMSIIFSSDWEIETGQHVLFPLLFNTHIVAAHKLISTGHTIQIIPSGPGC
PEGIIHQALLISLYEARKKLIITTPYLVPSDDLLHAICTAAQRGVEVHIIIPQNNDSVLVNWASRAFFSELLKAGVLIHQ
FQNGLLHVKSVLIDQQLSLIGTVNLDIRSLWLNFEITLVIDSKDFSNELEKTQENYIAHSKTIDPQKWANRPYWERIVER
LFYFLSPLL

Sequences:

>Translated_489_residues
MIAIHAIFNCIFLCSYWLLIVLVTFRVLIKRRAVPSSMAWLLVIYVLPLIGIITYLLFGESNLGKQRSNRSKIAWSSTMR
RIRILKTYKHIFSTEHSVIASSLFKLCEHRQGIGALKGNQIQLLKKTDDTIISLIKDIELAQHSIDMVFYIWESGGLVDH
VIKKLIIAAKRGIRCRIILDSAGCANFFSSSCPNIMRQAGVNIVEALHVNLLRVFLRRMDLRQHRKMILIDDHIAYTGSM
NMVDPQLFKKNIGIGQWVDIMVRIDGPAASAMSIIFSSDWEIETGQHVLFPLLFNTHIVAAHKLISTGHTIQIIPSGPGC
PEGIIHQALLISLYEARKKLIITTPYLVPSDDLLHAICTAAQRGVEVHIIIPQNNDSVLVNWASRAFFSELLKAGVLIHQ
FQNGLLHVKSVLIDQQLSLIGTVNLDIRSLWLNFEITLVIDSKDFSNELEKTQENYIAHSKTIDPQKWANRPYWERIVER
LFYFLSPLL
>Mature_489_residues
MIAIHAIFNCIFLCSYWLLIVLVTFRVLIKRRAVPSSMAWLLVIYVLPLIGIITYLLFGESNLGKQRSNRSKIAWSSTMR
RIRILKTYKHIFSTEHSVIASSLFKLCEHRQGIGALKGNQIQLLKKTDDTIISLIKDIELAQHSIDMVFYIWESGGLVDH
VIKKLIIAAKRGIRCRIILDSAGCANFFSSSCPNIMRQAGVNIVEALHVNLLRVFLRRMDLRQHRKMILIDDHIAYTGSM
NMVDPQLFKKNIGIGQWVDIMVRIDGPAASAMSIIFSSDWEIETGQHVLFPLLFNTHIVAAHKLISTGHTIQIIPSGPGC
PEGIIHQALLISLYEARKKLIITTPYLVPSDDLLHAICTAAQRGVEVHIIIPQNNDSVLVNWASRAFFSELLKAGVLIHQ
FQNGLLHVKSVLIDQQLSLIGTVNLDIRSLWLNFEITLVIDSKDFSNELEKTQENYIAHSKTIDPQKWANRPYWERIVER
LFYFLSPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=489, Percent_Identity=60.3271983640082, Blast_Score=642, Evalue=0.0,
Organism=Escherichia coli, GI1787007, Length=361, Percent_Identity=27.4238227146814, Blast_Score=119, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 55572; Mature: 55572

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAIHAIFNCIFLCSYWLLIVLVTFRVLIKRRAVPSSMAWLLVIYVLPLIGIITYLLFGE
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
SNLGKQRSNRSKIAWSSTMRRIRILKTYKHIFSTEHSVIASSLFKLCEHRQGIGALKGNQ
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCE
IQLLKKTDDTIISLIKDIELAQHSIDMVFYIWESGGLVDHVIKKLIIAAKRGIRCRIILD
EEEEECCHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEE
SAGCANFFSSSCPNIMRQAGVNIVEALHVNLLRVFLRRMDLRQHRKMILIDDHIAYTGSM
CCCHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEECCC
NMVDPQLFKKNIGIGQWVDIMVRIDGPAASAMSIIFSSDWEIETGQHVLFPLLFNTHIVA
CCCCHHHHHHCCCCCCEEEEEEEECCCHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHH
AHKLISTGHTIQIIPSGPGCPEGIIHQALLISLYEARKKLIITTPYLVPSDDLLHAICTA
HHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHH
AQRGVEVHIIIPQNNDSVLVNWASRAFFSELLKAGVLIHQFQNGLLHVKSVLIDQQLSLI
HHCCCEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
GTVNLDIRSLWLNFEITLVIDSKDFSNELEKTQENYIAHSKTIDPQKWANRPYWERIVER
EEECEEEEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHH
LFYFLSPLL
HHHHHHCCC
>Mature Secondary Structure
MIAIHAIFNCIFLCSYWLLIVLVTFRVLIKRRAVPSSMAWLLVIYVLPLIGIITYLLFGE
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
SNLGKQRSNRSKIAWSSTMRRIRILKTYKHIFSTEHSVIASSLFKLCEHRQGIGALKGNQ
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCE
IQLLKKTDDTIISLIKDIELAQHSIDMVFYIWESGGLVDHVIKKLIIAAKRGIRCRIILD
EEEEECCHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEE
SAGCANFFSSSCPNIMRQAGVNIVEALHVNLLRVFLRRMDLRQHRKMILIDDHIAYTGSM
CCCHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEECCC
NMVDPQLFKKNIGIGQWVDIMVRIDGPAASAMSIIFSSDWEIETGQHVLFPLLFNTHIVA
CCCCHHHHHHCCCCCCEEEEEEEECCCHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHH
AHKLISTGHTIQIIPSGPGCPEGIIHQALLISLYEARKKLIITTPYLVPSDDLLHAICTA
HHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHH
AQRGVEVHIIIPQNNDSVLVNWASRAFFSELLKAGVLIHQFQNGLLHVKSVLIDQQLSLI
HHCCCEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
GTVNLDIRSLWLNFEITLVIDSKDFSNELEKTQENYIAHSKTIDPQKWANRPYWERIVER
EEECEEEEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHH
LFYFLSPLL
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA