Click here to switch to the map view.

The map label for this gene is hns [H]

Identifier: 71892210

GI number: 71892210

Start: 531205

End: 531615

Strand: Direct

Name: hns [H]

Synonym: BPEN_448

Alternate gene names: 71892210

Gene position: 531205-531615 (Clockwise)

Preceding gene: 71892208

Following gene: 71892211

Centisome position: 67.1

GC content: 32.36

Gene sequence:

>411_bases
ATGAACGACACACTAAAAATTCTTAATAATATACGCACTTTACGAGCGCAAGCTAGAGCATGCACCCTTGATACCTTAGA
AGAAATGTTAGAAAAATTAGAAGTAGTTGTTAATGAACGCCGAGAAGAAAATAATCAAGCTCAAGCTGAAATAGAAAAAC
ATTCTCGAAAATTACAACAATATCGTGATATGCTCATAGCTGATGGGATAGATCCTAATGAACTATTACAAAATATAACT
TCTAATACTAAGCCTATTCATAAAAATAAACGTGCAGCTCGCCCAGCAAAGTATCAATACATTAACAAATATGGTGAGAT
TAAAACTTGGACCGGACAAGGACGTACTCCCTCAATTATCAAAAAAGCTATCGATGAAAAAAAGAAAAAATTAGAAGATT
TTTTACTATAA

Upstream 100 bases:

>100_bases
AGATTATTTATAAATTAAACACCCTAGGTCTTGCAGAAAATAAATTAGCGCTCTATCCTTATACTAGACTTATTATTTAT
CACATTTTGAGATTAATTTA

Downstream 100 bases:

>100_bases
TTAATGATAAATAATACTTATTTATTTCATCTCAAACAAAGAAATTCTTGGTAATATTTTCAAGCTCTTTGTCTGGAATC
ATCATATGCAGAATTTAATA

Product: global DNA-binding transcriptional dual regulator H-NS

Products: NA

Alternate protein names: Histone-like protein HLP-II [H]

Number of amino acids: Translated: 136; Mature: 136

Protein sequence:

>136_residues
MNDTLKILNNIRTLRAQARACTLDTLEEMLEKLEVVVNERREENNQAQAEIEKHSRKLQQYRDMLIADGIDPNELLQNIT
SNTKPIHKNKRAARPAKYQYINKYGEIKTWTGQGRTPSIIKKAIDEKKKKLEDFLL

Sequences:

>Translated_136_residues
MNDTLKILNNIRTLRAQARACTLDTLEEMLEKLEVVVNERREENNQAQAEIEKHSRKLQQYRDMLIADGIDPNELLQNIT
SNTKPIHKNKRAARPAKYQYINKYGEIKTWTGQGRTPSIIKKAIDEKKKKLEDFLL
>Mature_136_residues
MNDTLKILNNIRTLRAQARACTLDTLEEMLEKLEVVVNERREENNQAQAEIEKHSRKLQQYRDMLIADGIDPNELLQNIT
SNTKPIHKNKRAARPAKYQYINKYGEIKTWTGQGRTPSIIKKAIDEKKKKLEDFLL

Specific function: H-NS binds tightly to ds-DNA, increases its thermal stability and inhibits transcription. It also binds to ss-DNA and RNA but with a much lower affinity. H-NS has possible histone-like function. May be a global transcriptional regulator through its abilit

COG id: COG2916

COG function: function code R; DNA-binding protein H-NS

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the histone-like protein H-NS family [H]

Homologues:

Organism=Escherichia coli, GI1787489, Length=136, Percent_Identity=70.5882352941177, Blast_Score=176, Evalue=4e-46,
Organism=Escherichia coli, GI1789023, Length=136, Percent_Identity=51.4705882352941, Blast_Score=115, Evalue=1e-27,

Paralogues:

None

Copy number: 820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1780 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 1020 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 4632 Molecules/Cell In: Gr

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001801 [H]

Pfam domain/function: PF00816 Histone_HNS [H]

EC number: NA

Molecular weight: Translated: 15891; Mature: 15891

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDTLKILNNIRTLRAQARACTLDTLEEMLEKLEVVVNERREENNQAQAEIEKHSRKLQQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRDMLIADGIDPNELLQNITSNTKPIHKNKRAARPAKYQYINKYGEIKTWTGQGRTPSII
HHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHCCEEECCCCCCCHHHH
KKAIDEKKKKLEDFLL
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNDTLKILNNIRTLRAQARACTLDTLEEMLEKLEVVVNERREENNQAQAEIEKHSRKLQQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRDMLIADGIDPNELLQNITSNTKPIHKNKRAARPAKYQYINKYGEIKTWTGQGRTPSII
HHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHCCEEECCCCCCCHHHH
KKAIDEKKKKLEDFLL
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2494066 [H]