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The map label for this gene is nth [H]

Identifier: 71892150

GI number: 71892150

Start: 449664

End: 450305

Strand: Reverse

Name: nth [H]

Synonym: BPEN_383

Alternate gene names: 71892150

Gene position: 450305-449664 (Counterclockwise)

Preceding gene: 71892151

Following gene: 71892146

Centisome position: 56.88

GC content: 31.31

Gene sequence:

>642_bases
ATGAATCGCGTTAAACGTTATCAGATTTTATGCAAATTACGGGATAATAATATTCGTCCAGTTATTGAACTAGTTTATCG
GTCAGAATTTGAGTTGTTGATTGCAGTATTATTGTCGGCTCAAACAAGTGATGTACAAGTTAATAAAGTAACAACGAGTT
TGTTTAAAGTAGTTAATACACCCCAAGATATGTTACGTTTGGGCGTAGATGGGGTTAAAAATTATATTAAATCTATTGGT
TTATCAAATATTAAATCTAAAAATATTATTGAAACTTGTCGTTTGTTGATAGACAAATATAACGGTATATTACCGTCAAA
TCGTGTTGGTTTGGAATCTTTACCTGGAGTTGGACGTAAAACTGCTAATATTATTTTAAATGTAGTTTTCGATTGGCCTA
CAATTGCAGTAGATACACACGTATTTAGATTTTGTAATCGTAGTCGTTTTGCGTTAGGAAATACTGTTTTGTCTGTAGAA
AAGAAATTATTGTCGGTAGTACCAAAAGAATTTCAAAGAAACTGTCATCAGTGGTTAGTATTACATGGTAGGCATGTTTG
TCGAGCTAGACAACCAAATTGTCGTGTTTGTGTGATTAAAGATTTGTGTGAATTTAAGGAAAAAAAACGATTTATATTAT
AG

Upstream 100 bases:

>100_bases
TTAATTATTGCTAAAACTGAATAATATAAGTAGATTGAATAATAGTAAAAGTATTATAAGTATTTTTAATAAAACGTTAT
GAAATAATTAAGTGTTATTA

Downstream 100 bases:

>100_bases
TAGAAGTTACATAAATAAAATACTACGCATGGCGTAGTAAGGTATTGCGGGAAATTGTATTTATATTTTCAAACAATATA
AATAGTTATATACATAAAAC

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MNRVKRYQILCKLRDNNIRPVIELVYRSEFELLIAVLLSAQTSDVQVNKVTTSLFKVVNTPQDMLRLGVDGVKNYIKSIG
LSNIKSKNIIETCRLLIDKYNGILPSNRVGLESLPGVGRKTANIILNVVFDWPTIAVDTHVFRFCNRSRFALGNTVLSVE
KKLLSVVPKEFQRNCHQWLVLHGRHVCRARQPNCRVCVIKDLCEFKEKKRFIL

Sequences:

>Translated_213_residues
MNRVKRYQILCKLRDNNIRPVIELVYRSEFELLIAVLLSAQTSDVQVNKVTTSLFKVVNTPQDMLRLGVDGVKNYIKSIG
LSNIKSKNIIETCRLLIDKYNGILPSNRVGLESLPGVGRKTANIILNVVFDWPTIAVDTHVFRFCNRSRFALGNTVLSVE
KKLLSVVPKEFQRNCHQWLVLHGRHVCRARQPNCRVCVIKDLCEFKEKKRFIL
>Mature_213_residues
MNRVKRYQILCKLRDNNIRPVIELVYRSEFELLIAVLLSAQTSDVQVNKVTTSLFKVVNTPQDMLRLGVDGVKNYIKSIG
LSNIKSKNIIETCRLLIDKYNGILPSNRVGLESLPGVGRKTANIILNVVFDWPTIAVDTHVFRFCNRSRFALGNTVLSVE
KKLLSVVPKEFQRNCHQWLVLHGRHVCRARQPNCRVCVIKDLCEFKEKKRFIL

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=183, Percent_Identity=30.0546448087432, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1787920, Length=208, Percent_Identity=62.0192307692308, Blast_Score=287, Evalue=4e-79,
Organism=Caenorhabditis elegans, GI17554540, Length=176, Percent_Identity=30.1136363636364, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324530, Length=195, Percent_Identity=27.6923076923077, Blast_Score=69, Evalue=7e-13,
Organism=Drosophila melanogaster, GI45550361, Length=189, Percent_Identity=30.6878306878307, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24492; Mature: 24492

Theoretical pI: Translated: 10.53; Mature: 10.53

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRVKRYQILCKLRDNNIRPVIELVYRSEFELLIAVLLSAQTSDVQVNKVTTSLFKVVNT
CCCHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCC
PQDMLRLGVDGVKNYIKSIGLSNIKSKNIIETCRLLIDKYNGILPSNRVGLESLPGVGRK
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCHH
TANIILNVVFDWPTIAVDTHVFRFCNRSRFALGNTVLSVEKKLLSVVPKEFQRNCHQWLV
HHHHHHHHEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LHGRHVCRARQPNCRVCVIKDLCEFKEKKRFIL
HHHHHHHHCCCCCCCEEHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNRVKRYQILCKLRDNNIRPVIELVYRSEFELLIAVLLSAQTSDVQVNKVTTSLFKVVNT
CCCHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCC
PQDMLRLGVDGVKNYIKSIGLSNIKSKNIIETCRLLIDKYNGILPSNRVGLESLPGVGRK
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCHH
TANIILNVVFDWPTIAVDTHVFRFCNRSRFALGNTVLSVEKKLLSVVPKEFQRNCHQWLV
HHHHHHHHEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LHGRHVCRARQPNCRVCVIKDLCEFKEKKRFIL
HHHHHHHHCCCCCCCEEHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]