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The map label for this gene is rnt

Identifier: 71892146

GI number: 71892146

Start: 445293

End: 445946

Strand: Reverse

Name: rnt

Synonym: BPEN_379

Alternate gene names: 71892146

Gene position: 445946-445293 (Counterclockwise)

Preceding gene: 71892150

Following gene: 71892143

Centisome position: 56.33

GC content: 37.16

Gene sequence:

>654_bases
ATGATTTGTAAGGACGATCACAGTACTCTTCGAGCAAGATTTCGTGGGTTTTATCCAGTTGTAGTTGATGTAGAGACAGC
AGGTCTTGACGCTAATATTAATGCTTTATTAGAAATTGCTGTTGCTACATTTAAAATGGACGAATATGGTTGGTTGTGTG
TAGATGGTTCTCTTAATTTTCATATTAAATCATTTCCTGGAGCTATTATTCAGCCAGAATCATTGGCGTTTAATGGAATT
GATTTAAATAGTCCTTTAAGAGCGGCAGTGACGGAACAAGAAGCATTACATGAAATTTTCGAAATGGTGCGCCAAGGCAT
CAAAACTCAAGGATGTAGTCGTGCTATTATCGTAGCACACAATGCTTTTTTTGATCACAGTTTTTTAATGGCAGCAGTAG
AACGTGTAAGTTTTATGGAAAAAAATCCATTTCACCCTTTTGTTATGTTCGATACAGCTACTTTAAGTGGATTAGTATTT
GGGCAAACTGTATTAGCTAAAGCGTGTGCATGTGCGGGTGTTTTTTTTGATAAAAACAAAGCGCATTCCGCATTATATGA
CACTGAGCGCACCGCAATGTTATTTTGTGAACTGGTAAATAAATGGAAAAGACTTGGCGGTTGGCCATTAACTCCTTGTT
GTATCGAGTCTTAA

Upstream 100 bases:

>100_bases
AGTGTTCAAACAATCATTTTTACACGATAAATATAAAAATATAATTAGAATTTATGGTAGTATTAACTGTTTATATAATG
TGATATATCTCATAATAAAT

Downstream 100 bases:

>100_bases
TGCCGTTAAATGTTCGATATTTAGTTACGGCTTTCTTGTGTTATAAAGAAGAGTTATAAATTTGAATAAAAATATGTTTT
CATATTACACTTATCTAGGT

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MICKDDHSTLRARFRGFYPVVVDVETAGLDANINALLEIAVATFKMDEYGWLCVDGSLNFHIKSFPGAIIQPESLAFNGI
DLNSPLRAAVTEQEALHEIFEMVRQGIKTQGCSRAIIVAHNAFFDHSFLMAAVERVSFMEKNPFHPFVMFDTATLSGLVF
GQTVLAKACACAGVFFDKNKAHSALYDTERTAMLFCELVNKWKRLGGWPLTPCCIES

Sequences:

>Translated_217_residues
MICKDDHSTLRARFRGFYPVVVDVETAGLDANINALLEIAVATFKMDEYGWLCVDGSLNFHIKSFPGAIIQPESLAFNGI
DLNSPLRAAVTEQEALHEIFEMVRQGIKTQGCSRAIIVAHNAFFDHSFLMAAVERVSFMEKNPFHPFVMFDTATLSGLVF
GQTVLAKACACAGVFFDKNKAHSALYDTERTAMLFCELVNKWKRLGGWPLTPCCIES
>Mature_217_residues
MICKDDHSTLRARFRGFYPVVVDVETAGLDANINALLEIAVATFKMDEYGWLCVDGSLNFHIKSFPGAIIQPESLAFNGI
DLNSPLRAAVTEQEALHEIFEMVRQGIKTQGCSRAIIVAHNAFFDHSFLMAAVERVSFMEKNPFHPFVMFDTATLSGLVF
GQTVLAKACACAGVFFDKNKAHSALYDTERTAMLFCELVNKWKRLGGWPLTPCCIES

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain

Homologues:

Organism=Escherichia coli, GI1787941, Length=199, Percent_Identity=69.3467336683417, Blast_Score=297, Evalue=4e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNT_BLOPB (Q492U0)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277878.1
- HSSP:   Q9HY82
- ProteinModelPortal:   Q492U0
- SMR:   Q492U0
- STRING:   Q492U0
- GeneID:   3562978
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_379
- eggNOG:   COG0847
- HOGENOM:   HBG289562
- OMA:   YDAEKTA
- ProtClustDB:   PRK05168
- BioCyc:   CBLO291272:BPEN_379-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00157
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337
- SMART:   SM00479
- TIGRFAMs:   TIGR01298

Pfam domain/function: PF00929 Exonuc_X-T; SSF53098 RNaseH_fold

EC number: 3.1.13.-

Molecular weight: Translated: 24107; Mature: 24107

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MICKDDHSTLRARFRGFYPVVVDVETAGLDANINALLEIAVATFKMDEYGWLCVDGSLNF
CCCCCCHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHEEECCCCEEEECCCEEE
HIKSFPGAIIQPESLAFNGIDLNSPLRAAVTEQEALHEIFEMVRQGIKTQGCSRAIIVAH
EEECCCCCEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
NAFFDHSFLMAAVERVSFMEKNPFHPFVMFDTATLSGLVFGQTVLAKACACAGVFFDKNK
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHEECCCC
AHSALYDTERTAMLFCELVNKWKRLGGWPLTPCCIES
CCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHCCCC
>Mature Secondary Structure
MICKDDHSTLRARFRGFYPVVVDVETAGLDANINALLEIAVATFKMDEYGWLCVDGSLNF
CCCCCCHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHEEECCCCEEEECCCEEE
HIKSFPGAIIQPESLAFNGIDLNSPLRAAVTEQEALHEIFEMVRQGIKTQGCSRAIIVAH
EEECCCCCEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
NAFFDHSFLMAAVERVSFMEKNPFHPFVMFDTATLSGLVFGQTVLAKACACAGVFFDKNK
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHEECCCC
AHSALYDTERTAMLFCELVNKWKRLGGWPLTPCCIES
CCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA