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The map label for this gene is hemK [H]

Identifier: 71892128

GI number: 71892128

Start: 425049

End: 425885

Strand: Direct

Name: hemK [H]

Synonym: BPEN_360

Alternate gene names: 71892128

Gene position: 425049-425885 (Clockwise)

Preceding gene: 71892127

Following gene: 71892129

Centisome position: 53.69

GC content: 31.42

Gene sequence:

>837_bases
ATGACATGGGATCAATGGTTACATTGGGCCAATTTAAAACTAAAAAAATCCATCAGCCCAAAAAGAGATGCAGAGATTAT
TTTAGGTCAGGTTACCAAAAAATCTCGCGCTCAATTGTTAGCATTTGGAGAATCATTATTAGAATATGACACTATTATCC
AGTTAAAATCTTTAATCTATCGAAGAAAAAAAGGAGAGCCTATAGCTTATCTCGTTGGTTCAAAGGAATTTTGGTCCTTA
GATCTTAAAGTTTCGCCAGGTACATTCATTCCTAGACCAGATACTGAATGCTTAGTAAAGCATGTATTCGATTTATTAAA
GGTATCTCATTTAAATGTTTTAGACTTAGGGACCGGAGTAGGTACTATAGCTTTAGCTCTTGCATCAGAACGACCAAACT
GGAATATTACAGGAATAGACTGTCAAAAAAAAGCATTATTTCTTGCTCATAAAAATAAGCTGTTACTTAATTTTAAAAAT
GTAAAATTTATATATGGAGATTGGTTTAAATATCTAAGAAATAAAAAATTTAATCTCATTGTTAGCAATCCACCATATAT
CGATAAAAATGATTCATGTTTACAATTCAGAGATATGATTTTTGAACCTAAAAATGCATTAGTATCAAAACAAAAAGGTT
TAGAAGATTTAACAGTAATTTGCAAATATTCCACTCAACATTTGCGTCAGAATGGATGGCTGGTGTTGGAACATGGATGG
AATCAAGGAAATTATATGCGCGCGTTATTTTTTAAATTTGGATTTACTCATATCCATACAATACGTGATTATCATCATTA
TGAACGCGTAACATACGGAAAATGGAAATCCCATTAA

Upstream 100 bases:

>100_bases
AATTAAATGAAATAATGAACGGAGAATTAGATCTCTTGATACAACCCATCATTCATCAACATCAATCCGATCAACTTAAT
AAATTATTAGAATTAGAATA

Downstream 100 bases:

>100_bases
AATATTATATTAATAAACGCAAATAAATAGTACAATATTTAATTGACAGAATAATGTAAGTTAAATAAATAAATTAAAAA
CCAATAAACATTACAACTTG

Product: N5-glutamine methyltransferase

Products: NA

Alternate protein names: M.StyLTHemKP; Protein-(glutamine-N(5)) MTase hemK; Protein-glutamine N-methyltransferase hemK [H]

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MTWDQWLHWANLKLKKSISPKRDAEIILGQVTKKSRAQLLAFGESLLEYDTIIQLKSLIYRRKKGEPIAYLVGSKEFWSL
DLKVSPGTFIPRPDTECLVKHVFDLLKVSHLNVLDLGTGVGTIALALASERPNWNITGIDCQKKALFLAHKNKLLLNFKN
VKFIYGDWFKYLRNKKFNLIVSNPPYIDKNDSCLQFRDMIFEPKNALVSKQKGLEDLTVICKYSTQHLRQNGWLVLEHGW
NQGNYMRALFFKFGFTHIHTIRDYHHYERVTYGKWKSH

Sequences:

>Translated_278_residues
MTWDQWLHWANLKLKKSISPKRDAEIILGQVTKKSRAQLLAFGESLLEYDTIIQLKSLIYRRKKGEPIAYLVGSKEFWSL
DLKVSPGTFIPRPDTECLVKHVFDLLKVSHLNVLDLGTGVGTIALALASERPNWNITGIDCQKKALFLAHKNKLLLNFKN
VKFIYGDWFKYLRNKKFNLIVSNPPYIDKNDSCLQFRDMIFEPKNALVSKQKGLEDLTVICKYSTQHLRQNGWLVLEHGW
NQGNYMRALFFKFGFTHIHTIRDYHHYERVTYGKWKSH
>Mature_277_residues
TWDQWLHWANLKLKKSISPKRDAEIILGQVTKKSRAQLLAFGESLLEYDTIIQLKSLIYRRKKGEPIAYLVGSKEFWSLD
LKVSPGTFIPRPDTECLVKHVFDLLKVSHLNVLDLGTGVGTIALALASERPNWNITGIDCQKKALFLAHKNKLLLNFKNV
KFIYGDWFKYLRNKKFNLIVSNPPYIDKNDSCLQFRDMIFEPKNALVSKQKGLEDLTVICKYSTQHLRQNGWLVLEHGWN
QGNYMRALFFKFGFTHIHTIRDYHHYERVTYGKWKSH

Specific function: Methylates the translation termination release factor RF1 on 'Gln-235' and RF2 on 'Gln-252' [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=250, Percent_Identity=28.8, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1787463, Length=275, Percent_Identity=48, Blast_Score=268, Evalue=3e-73,
Organism=Escherichia coli, GI87082085, Length=187, Percent_Identity=29.4117647058824, Blast_Score=79, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324266, Length=163, Percent_Identity=30.6748466257669, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24582226, Length=193, Percent_Identity=31.0880829015544, Blast_Score=95, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 32514; Mature: 32383

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWDQWLHWANLKLKKSISPKRDAEIILGQVTKKSRAQLLAFGESLLEYDTIIQLKSLIY
CCHHHHHHCCCEEEECCCCCCCCHHEEEEHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRKKGEPIAYLVGSKEFWSLDLKVSPGTFIPRPDTECLVKHVFDLLKVSHLNVLDLGTGV
HHCCCCCEEEEECCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCH
GTIALALASERPNWNITGIDCQKKALFLAHKNKLLLNFKNVKFIYGDWFKYLRNKKFNLI
HHEEEEEECCCCCCCEEEECCCCCEEEEEECCEEEEEECCEEEEHHHHHHHHCCCCEEEE
VSNPPYIDKNDSCLQFRDMIFEPKNALVSKQKGLEDLTVICKYSTQHLRQNGWLVLEHGW
ECCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCHHHHEEEHHHHHHHHCCCEEEEEECC
NQGNYMRALFFKFGFTHIHTIRDYHHYERVTYGKWKSH
CCCCHHHHHHHHHCHHHHHHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure 
TWDQWLHWANLKLKKSISPKRDAEIILGQVTKKSRAQLLAFGESLLEYDTIIQLKSLIY
CHHHHHHCCCEEEECCCCCCCCHHEEEEHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRKKGEPIAYLVGSKEFWSLDLKVSPGTFIPRPDTECLVKHVFDLLKVSHLNVLDLGTGV
HHCCCCCEEEEECCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCH
GTIALALASERPNWNITGIDCQKKALFLAHKNKLLLNFKNVKFIYGDWFKYLRNKKFNLI
HHEEEEEECCCCCCCEEEECCCCCEEEEEECCEEEEEECCEEEEHHHHHHHHCCCCEEEE
VSNPPYIDKNDSCLQFRDMIFEPKNALVSKQKGLEDLTVICKYSTQHLRQNGWLVLEHGW
ECCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCHHHHEEEHHHHHHHHCCCEEEEEECC
NQGNYMRALFFKFGFTHIHTIRDYHHYERVTYGKWKSH
CCCCHHHHHHHHHCHHHHHHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 2544564 [H]