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The map label for this gene is prfA

Identifier: 71892127

GI number: 71892127

Start: 423967

End: 425049

Strand: Direct

Name: prfA

Synonym: BPEN_359

Alternate gene names: 71892127

Gene position: 423967-425049 (Clockwise)

Preceding gene: 71892121

Following gene: 71892128

Centisome position: 53.55

GC content: 34.35

Gene sequence:

>1083_bases
ATGAATTCTATCATTGTCAATAAACTAATAGCTTTACAGGAACGTTTTAATGTATTAGAAAAATTACTCAGTAAACCCAA
TGCTATTGATGACAAAAAACATTTTTGTACATTAGCTAAGGAACACGCTCGACTATCTGAAATAGTTGTTTGTTTTGAAC
GTTGGTTGGATATAAAACAAGAAATAATTAATACAAAAAAATTGCTTGAAGATGTAGATATGCATGATATTGCTCAAGAT
GAGCTAAAAACATTCTATTTAAATCGGAATAATATTGAAAAAAACTTAAAAATACTACTATTACCCACTGATTCTAATGA
TAAGCTAGGTTGTTTTATTGAACTACGGGCTGGCACTGGAGGAAAAGAAGCAGCAATTTTTACGGGAGAATTATTTCGAA
TGTATGTTCGTTATTCAGAGGTTCGTCGATGGAAAATGGAAATTATCAATGCTACTTATGGAGAATGCGGTGGTTATAAA
GAAATTATTGCTAAAATTCCTTATAGAGGTGCATATAGTCTCTTAAAATTCGAATCTGGAGGACACCGCGTTCAAAGAAT
ACCGCATACTGAATCTCAAGGTCGCATTCATACTTCCACTTGCACAATCGCTGTAATTCCAGAGATACCAGATATCGAAT
TGCCTAGTATTGATCCTCATGATTTAAGAATCGATACCTTTCGGTCATCAGGTGCAGGAGGACAACATGTAAATACTACC
GATTCAGCAATCCGTATTACCCATGTACCAAGTGGATTGGTTGTGGAATGCCAAGACGAACGCTCACAACATAAAAATAA
AGCCAAAGCATTATCAGTACTTGGTTCGCGATTACATGCTATCGAAATGAAACGTCGACAACAAGAAGTATCTTGCACCC
GACGTAACTTACTAGGTACTGGGGATCGATCTGATCGAATTCGTACATATAATTTTCAACAAGGCCGAATTACTGATCAT
CGTATTTCTTTTACATCATATAAATTAAATGAAATAATGAACGGAGAATTAGATCTCTTGATACAACCCATCATTCATCA
ACATCAATCCGATCAACTTAATAAATTATTAGAATTAGAATAA

Upstream 100 bases:

>100_bases
ATCTTCATATATACAATATATATTTAAATTATATTGATAATTATTGATGTTATCCATTTTTATCTAAACAAAAATCCACC
TCTCCTTAAGATAAAGATAT

Downstream 100 bases:

>100_bases
TGACATGGGATCAATGGTTACATTGGGCCAATTTAAAACTAAAAAAATCCATCAGCCCAAAAAGAGATGCAGAGATTATT
TTAGGTCAGGTTACCAAAAA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MNSIIVNKLIALQERFNVLEKLLSKPNAIDDKKHFCTLAKEHARLSEIVVCFERWLDIKQEIINTKKLLEDVDMHDIAQD
ELKTFYLNRNNIEKNLKILLLPTDSNDKLGCFIELRAGTGGKEAAIFTGELFRMYVRYSEVRRWKMEIINATYGECGGYK
EIIAKIPYRGAYSLLKFESGGHRVQRIPHTESQGRIHTSTCTIAVIPEIPDIELPSIDPHDLRIDTFRSSGAGGQHVNTT
DSAIRITHVPSGLVVECQDERSQHKNKAKALSVLGSRLHAIEMKRRQQEVSCTRRNLLGTGDRSDRIRTYNFQQGRITDH
RISFTSYKLNEIMNGELDLLIQPIIHQHQSDQLNKLLELE

Sequences:

>Translated_360_residues
MNSIIVNKLIALQERFNVLEKLLSKPNAIDDKKHFCTLAKEHARLSEIVVCFERWLDIKQEIINTKKLLEDVDMHDIAQD
ELKTFYLNRNNIEKNLKILLLPTDSNDKLGCFIELRAGTGGKEAAIFTGELFRMYVRYSEVRRWKMEIINATYGECGGYK
EIIAKIPYRGAYSLLKFESGGHRVQRIPHTESQGRIHTSTCTIAVIPEIPDIELPSIDPHDLRIDTFRSSGAGGQHVNTT
DSAIRITHVPSGLVVECQDERSQHKNKAKALSVLGSRLHAIEMKRRQQEVSCTRRNLLGTGDRSDRIRTYNFQQGRITDH
RISFTSYKLNEIMNGELDLLIQPIIHQHQSDQLNKLLELE
>Mature_360_residues
MNSIIVNKLIALQERFNVLEKLLSKPNAIDDKKHFCTLAKEHARLSEIVVCFERWLDIKQEIINTKKLLEDVDMHDIAQD
ELKTFYLNRNNIEKNLKILLLPTDSNDKLGCFIELRAGTGGKEAAIFTGELFRMYVRYSEVRRWKMEIINATYGECGGYK
EIIAKIPYRGAYSLLKFESGGHRVQRIPHTESQGRIHTSTCTIAVIPEIPDIELPSIDPHDLRIDTFRSSGAGGQHVNTT
DSAIRITHVPSGLVVECQDERSQHKNKAKALSVLGSRLHAIEMKRRQQEVSCTRRNLLGTGDRSDRIRTYNFQQGRITDH
RISFTSYKLNEIMNGELDLLIQPIIHQHQSDQLNKLLELE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=305, Percent_Identity=42.2950819672131, Blast_Score=248, Evalue=8e-66,
Organism=Homo sapiens, GI34577120, Length=362, Percent_Identity=37.8453038674033, Blast_Score=225, Evalue=4e-59,
Organism=Homo sapiens, GI166795305, Length=210, Percent_Identity=42.3809523809524, Blast_Score=174, Evalue=1e-43,
Organism=Escherichia coli, GI1787462, Length=360, Percent_Identity=61.9444444444444, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI2367172, Length=339, Percent_Identity=35.1032448377581, Blast_Score=202, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI17542784, Length=304, Percent_Identity=35.1973684210526, Blast_Score=181, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6321295, Length=329, Percent_Identity=41.3373860182371, Blast_Score=238, Evalue=1e-63,
Organism=Drosophila melanogaster, GI19921226, Length=338, Percent_Identity=38.1656804733728, Blast_Score=213, Evalue=2e-55,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_BLOPB (Q492V8)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277859.1
- HSSP:   P0A7I0
- ProteinModelPortal:   Q492V8
- SMR:   Q492V8
- STRING:   Q492V8
- GeneID:   3563033
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_359
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   NTNEGEH
- ProtClustDB:   PRK00591
- BioCyc:   CBLO291272:BPEN_359-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 41285; Mature: 41285

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSIIVNKLIALQERFNVLEKLLSKPNAIDDKKHFCTLAKEHARLSEIVVCFERWLDIKQ
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIINTKKLLEDVDMHDIAQDELKTFYLNRNNIEKNLKILLLPTDSNDKLGCFIELRAGTG
HHHHHHHHHHHCCHHHHHHHHHHEEEECCCCCCCCCEEEEEECCCCCCEEEEEEEECCCC
GKEAAIFTGELFRMYVRYSEVRRWKMEIINATYGECGGYKEIIAKIPYRGAYSLLKFESG
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEEEEECCC
GHRVQRIPHTESQGRIHTSTCTIAVIPEIPDIELPSIDPHDLRIDTFRSSGAGGQHVNTT
CCEEEECCCCCCCCEEEECEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
DSAIRITHVPSGLVVECQDERSQHKNKAKALSVLGSRLHAIEMKRRQQEVSCTRRNLLGT
CCEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSDRIRTYNFQQGRITDHRISFTSYKLNEIMNGELDLLIQPIIHQHQSDQLNKLLELE
CCCCCCEEEEECCCCCCCCCEEEEHHEEHHHHCCCCHHHHHHHHHHCCCHHHHHHHHCCC
>Mature Secondary Structure
MNSIIVNKLIALQERFNVLEKLLSKPNAIDDKKHFCTLAKEHARLSEIVVCFERWLDIKQ
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIINTKKLLEDVDMHDIAQDELKTFYLNRNNIEKNLKILLLPTDSNDKLGCFIELRAGTG
HHHHHHHHHHHCCHHHHHHHHHHEEEECCCCCCCCCEEEEEECCCCCCEEEEEEEECCCC
GKEAAIFTGELFRMYVRYSEVRRWKMEIINATYGECGGYKEIIAKIPYRGAYSLLKFESG
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEEEEECCC
GHRVQRIPHTESQGRIHTSTCTIAVIPEIPDIELPSIDPHDLRIDTFRSSGAGGQHVNTT
CCEEEECCCCCCCCEEEECEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
DSAIRITHVPSGLVVECQDERSQHKNKAKALSVLGSRLHAIEMKRRQQEVSCTRRNLLGT
CCEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSDRIRTYNFQQGRITDHRISFTSYKLNEIMNGELDLLIQPIIHQHQSDQLNKLLELE
CCCCCCEEEEECCCCCCCCCEEEEHHEEHHHHCCCCHHHHHHHHHHCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA