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The map label for this gene is tolA [H]

Identifier: 71892115

GI number: 71892115

Start: 410688

End: 411872

Strand: Direct

Name: tolA [H]

Synonym: BPEN_347

Alternate gene names: 71892115

Gene position: 410688-411872 (Clockwise)

Preceding gene: 71892114

Following gene: 71892116

Centisome position: 51.88

GC content: 33.25

Gene sequence:

>1185_bases
TTGTTTAATAGATATTACAAAAAATTTAGATCTGCTGTTATGATATCAATCATTGTACATGTGATCGTCTCTTTATTGCT
ATATAAACATATAATAAAAAAACAACAACAATACACTGCTAAAATAGCTAATAACAAAAATTCAATTAATACCTTCATTC
ACAACTCTAACCCGAAAAAAGAAAAAAACAACAACATAAAAGATCAATCAAATCGATTCAAAATAACAGAAGCACAGCCT
CCATTGGCAGGACCAACAGCGCCCAACACCCCTAAAAAGAAAAGTCAAGATCAATCAAATCGATTCAAAATAACAGAAGC
ACAGCCTCCATTGGCAGGACCAACAGCGCCCAACACCCCTAAAAAGAAAAGTCAAGATCAATCAAATCGATTCAAAATAA
CAGAAGCACAGCCTCCATTGGCAGGACCAACAGCGCCCAACACCCCTAAAAAGAAAAGTCAAGATCAATCAAATCGATTC
AAAATAACAGAAGCACAGCCTCCATTGGCAGGACCAACAGCGCCCAACACCCCTAAAAAGAAAAGTCAAGATCAATCAAA
TCGATTCAAAATAACAGAAGCACAGCCTCCATTGGCAGGACCAACAGCGCCCAACACCCCTAAAAAGAAAAGTCAAGATC
AATCAAATCGATTCAAAATAACAGAAGCACAGCCTCCATTGGCAGGACCAACAGCGCCCAACACCCCTAAAAAGAAAAGT
CAGAATACTATAGAAAAAAATATACTAATACATGATAAATTAAATTATCAATCCCATGACTCTAACGAACTCAATAATTT
ACTGAATACATTAATTAACAAAAAAAAATCTATAGAAAATAACAAAAAATCTAAATTAGACTTGACAACAAAAAAAAATG
ATAATGAAAATAATATTTCAAACGAAATGATTAAAAGTAATGAAATTAATATATATAAGCGTATGATCAGCGAATCAATA
CAACAAAAATTTTATAACTTTTCGTATTATGTTGGTAAACAATGCAATCTACGCATCAAATTAGCACCTGACGGCACGTT
ATTATCAGTAACCGCTATATCTGGAGATTACGATTTATGCCAAGCAGCAATTATCGCCGCCAAATTAGCAAAAATTCCTA
AACCACCAAATTCAGATATTTACGAAATATTTAAAAACACAATATTAAATTTTTCTCCTCAATAA

Upstream 100 bases:

>100_bases
GTTAAATAAGATCGGTATAAATTCAATTGGAATGATAACAAATCCTTCCGTTTGAATACGGAACGATATTCTTACACAAC
TTGGATGATAATTATTAAAT

Downstream 100 bases:

>100_bases
ACAAAATATTTACACATTTATAAAATAATAATAATATATTGGTCCATTAAATAATCTATTTTCATTATAAAATTTTCATG
TATTTAATAATTAAAAAAAC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MFNRYYKKFRSAVMISIIVHVIVSLLLYKHIIKKQQQYTAKIANNKNSINTFIHNSNPKKEKNNNIKDQSNRFKITEAQP
PLAGPTAPNTPKKKSQDQSNRFKITEAQPPLAGPTAPNTPKKKSQDQSNRFKITEAQPPLAGPTAPNTPKKKSQDQSNRF
KITEAQPPLAGPTAPNTPKKKSQDQSNRFKITEAQPPLAGPTAPNTPKKKSQDQSNRFKITEAQPPLAGPTAPNTPKKKS
QNTIEKNILIHDKLNYQSHDSNELNNLLNTLINKKKSIENNKKSKLDLTTKKNDNENNISNEMIKSNEINIYKRMISESI
QQKFYNFSYYVGKQCNLRIKLAPDGTLLSVTAISGDYDLCQAAIIAAKLAKIPKPPNSDIYEIFKNTILNFSPQ

Sequences:

>Translated_394_residues
MFNRYYKKFRSAVMISIIVHVIVSLLLYKHIIKKQQQYTAKIANNKNSINTFIHNSNPKKEKNNNIKDQSNRFKITEAQP
PLAGPTAPNTPKKKSQDQSNRFKITEAQPPLAGPTAPNTPKKKSQDQSNRFKITEAQPPLAGPTAPNTPKKKSQDQSNRF
KITEAQPPLAGPTAPNTPKKKSQDQSNRFKITEAQPPLAGPTAPNTPKKKSQDQSNRFKITEAQPPLAGPTAPNTPKKKS
QNTIEKNILIHDKLNYQSHDSNELNNLLNTLINKKKSIENNKKSKLDLTTKKNDNENNISNEMIKSNEINIYKRMISESI
QQKFYNFSYYVGKQCNLRIKLAPDGTLLSVTAISGDYDLCQAAIIAAKLAKIPKPPNSDIYEIFKNTILNFSPQ
>Mature_394_residues
MFNRYYKKFRSAVMISIIVHVIVSLLLYKHIIKKQQQYTAKIANNKNSINTFIHNSNPKKEKNNNIKDQSNRFKITEAQP
PLAGPTAPNTPKKKSQDQSNRFKITEAQPPLAGPTAPNTPKKKSQDQSNRFKITEAQPPLAGPTAPNTPKKKSQDQSNRF
KITEAQPPLAGPTAPNTPKKKSQDQSNRFKITEAQPPLAGPTAPNTPKKKSQDQSNRFKITEAQPPLAGPTAPNTPKKKS
QNTIEKNILIHDKLNYQSHDSNELNNLLNTLINKKKSIENNKKSKLDLTTKKNDNENNISNEMIKSNEINIYKRMISESI
QQKFYNFSYYVGKQCNLRIKLAPDGTLLSVTAISGDYDLCQAAIIAAKLAKIPKPPNSDIYEIFKNTILNFSPQ

Specific function: Involved in the tonB-independent uptake of group A colicins (colicins A, E1, E2, E3, and K). Necessary for the colicins to reach their respective targets after initial binding to the bacteria. Also involved in the translocation of bacteriophage DNA [H]

COG id: COG3064

COG function: function code M; Membrane protein involved in colicin uptake

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786960, Length=97, Percent_Identity=53.6082474226804, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014161 [H]

Pfam domain/function: PF06519 TolA [H]

EC number: NA

Molecular weight: Translated: 44268; Mature: 44268

Theoretical pI: Translated: 10.68; Mature: 10.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNRYYKKFRSAVMISIIVHVIVSLLLYKHIIKKQQQYTAKIANNKNSINTFIHNSNPKK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCH
EKNNNIKDQSNRFKITEAQPPLAGPTAPNTPKKKSQDQSNRFKITEAQPPLAGPTAPNTP
HCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCC
KKKSQDQSNRFKITEAQPPLAGPTAPNTPKKKSQDQSNRFKITEAQPPLAGPTAPNTPKK
CCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCC
KSQDQSNRFKITEAQPPLAGPTAPNTPKKKSQDQSNRFKITEAQPPLAGPTAPNTPKKKS
CCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHH
QNTIEKNILIHDKLNYQSHDSNELNNLLNTLINKKKSIENNKKSKLDLTTKKNDNENNIS
HHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHH
NEMIKSNEINIYKRMISESIQQKFYNFSYYVGKQCNLRIKLAPDGTLLSVTAISGDYDLC
HHHHHCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEECCCCCEEEEEEECCCHHHH
QAAIIAAKLAKIPKPPNSDIYEIFKNTILNFSPQ
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFNRYYKKFRSAVMISIIVHVIVSLLLYKHIIKKQQQYTAKIANNKNSINTFIHNSNPKK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCH
EKNNNIKDQSNRFKITEAQPPLAGPTAPNTPKKKSQDQSNRFKITEAQPPLAGPTAPNTP
HCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCC
KKKSQDQSNRFKITEAQPPLAGPTAPNTPKKKSQDQSNRFKITEAQPPLAGPTAPNTPKK
CCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCC
KSQDQSNRFKITEAQPPLAGPTAPNTPKKKSQDQSNRFKITEAQPPLAGPTAPNTPKKKS
CCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHH
QNTIEKNILIHDKLNYQSHDSNELNNLLNTLINKKKSIENNKKSKLDLTTKKNDNENNIS
HHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHH
NEMIKSNEINIYKRMISESIQQKFYNFSYYVGKQCNLRIKLAPDGTLLSVTAISGDYDLC
HHHHHCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEECCCCCEEEEEEECCCHHHH
QAAIIAAKLAKIPKPPNSDIYEIFKNTILNFSPQ
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2687247; 8905232; 9278503; 2068069; 8978668; 10404600 [H]