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The map label for this gene is tolB

Identifier: 71892116

GI number: 71892116

Start: 411950

End: 413311

Strand: Direct

Name: tolB

Synonym: BPEN_348

Alternate gene names: 71892116

Gene position: 411950-413311 (Clockwise)

Preceding gene: 71892115

Following gene: 71892117

Centisome position: 52.04

GC content: 31.57

Gene sequence:

>1362_bases
ATGTATTTAATAATTAAAAAAACTCATAAACTACCACACTGGTTGCAAAAAGTATCTTTATCAATAATGTTAATTATATT
TTTATGGAAACCTGCGTTACTTGCAGATATGCATATCGAAATTACATGTGGAGTAAATGCTGCGCACCCCATTGCTGTAA
TACCTTTTACATGTGTTAGTAATCAGTATAACAAATCAATATCAATTGAAGATATAGCATCAATAATAGCCGCGGACTTA
CGCAATAGCAGCAAATTCAATACTATTCCCGTAGAATATTTACCACATAAACCCACTAAAGTATCCGATGTTATCCCAAC
TTTTTGGGAGAAACTGGGTATTAATATTATTGTATTAGGAACAGTACATATAAACTACGATGAAAGTTACATTATCTCAT
ATCATTTAATAGATACTTCCAGCAACCCTGCATTAATAATATCAGAAAATCAATATTCAGTAGAAAAAAAATGGCTACGC
TATGTAGCCCATGCTATAAGCAATGAAATTTTTGAAAAGTTAACCGGTATAAAAGGTGCATTTTGTACACGTATTGCTTA
TGTTTTGCGCATTCATAATGATCACTATCCCTATGAGTTATATATTTCTGATTACGATGGTCATAATCAAATTTCAATTT
GCCGCTCGACAGAACCATTAATGTCTCCGGCTTGGTCTCCAGATGGAAAAAAAATTGCTTATGTTACCTTTGCCTCCGGC
CATTCAGAACTTGTTATTCAAGCATTAAACACTGGGTTAGTTAATAATATTGTTAGTTTCCCGGGCCATAATGGCGCCCC
TTCTTTTTCTCCCGACTGCAAAAAACTAGCTTTTTCTTTATCCAAAACAGGTAGTTTAAATTTATATATTATGGATTTAG
AATCTGGAGAAATTACACAATTAACTAAAAATAGGAACAATAATACCGAACCTAGTTGGTTTCCGGATAATCAAAATATA
GCCTATACTTCAGACCAAGGAGGCAGTCCACAAATATATAAAATTAATGTTAAAACTACTGAAATTCAAAGATTATCTTG
GCTACATACTAGTAATCAAAATCCAAATGTTAGTTCAGATGGGACATTTATAATAATGGTTAATAGACATCAAGGAAAAC
AAAATATTGCTAAATTAAATTTATTAACTGGACAAGAAGAAATATTAACAGATACACTGTTAGCTGATTCTCCTAGTATA
GCGCCCAACAATACTATGGTATTATACAGTAATATTAATAAGGATTTAACAACAAAATCCAATTTAGAATTAATCTCTAT
TGATGGTCATTTTAAAGCTCATATACAAGGAGATCAAGGAGATATAAGATTTCCAACTTGGTCTCCATTACATTTAGAAT
AA

Upstream 100 bases:

>100_bases
TATTAAATTTTTCTCCTCAATAAACAAAATATTTACACATTTATAAAATAATAATAATATATTGGTCCATTAAATAATCT
ATTTTCATTATAAAATTTTC

Downstream 100 bases:

>100_bases
GATAATAACTATTTTCATTACGATTAACAATTTAAAAATAATGAACAATGGATATATAAATGAAACCAAACAATTTTTTT
AAACAACTAATATCCGCGAT

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MYLIIKKTHKLPHWLQKVSLSIMLIIFLWKPALLADMHIEITCGVNAAHPIAVIPFTCVSNQYNKSISIEDIASIIAADL
RNSSKFNTIPVEYLPHKPTKVSDVIPTFWEKLGINIIVLGTVHINYDESYIISYHLIDTSSNPALIISENQYSVEKKWLR
YVAHAISNEIFEKLTGIKGAFCTRIAYVLRIHNDHYPYELYISDYDGHNQISICRSTEPLMSPAWSPDGKKIAYVTFASG
HSELVIQALNTGLVNNIVSFPGHNGAPSFSPDCKKLAFSLSKTGSLNLYIMDLESGEITQLTKNRNNNTEPSWFPDNQNI
AYTSDQGGSPQIYKINVKTTEIQRLSWLHTSNQNPNVSSDGTFIIMVNRHQGKQNIAKLNLLTGQEEILTDTLLADSPSI
APNNTMVLYSNINKDLTTKSNLELISIDGHFKAHIQGDQGDIRFPTWSPLHLE

Sequences:

>Translated_453_residues
MYLIIKKTHKLPHWLQKVSLSIMLIIFLWKPALLADMHIEITCGVNAAHPIAVIPFTCVSNQYNKSISIEDIASIIAADL
RNSSKFNTIPVEYLPHKPTKVSDVIPTFWEKLGINIIVLGTVHINYDESYIISYHLIDTSSNPALIISENQYSVEKKWLR
YVAHAISNEIFEKLTGIKGAFCTRIAYVLRIHNDHYPYELYISDYDGHNQISICRSTEPLMSPAWSPDGKKIAYVTFASG
HSELVIQALNTGLVNNIVSFPGHNGAPSFSPDCKKLAFSLSKTGSLNLYIMDLESGEITQLTKNRNNNTEPSWFPDNQNI
AYTSDQGGSPQIYKINVKTTEIQRLSWLHTSNQNPNVSSDGTFIIMVNRHQGKQNIAKLNLLTGQEEILTDTLLADSPSI
APNNTMVLYSNINKDLTTKSNLELISIDGHFKAHIQGDQGDIRFPTWSPLHLE
>Mature_453_residues
MYLIIKKTHKLPHWLQKVSLSIMLIIFLWKPALLADMHIEITCGVNAAHPIAVIPFTCVSNQYNKSISIEDIASIIAADL
RNSSKFNTIPVEYLPHKPTKVSDVIPTFWEKLGINIIVLGTVHINYDESYIISYHLIDTSSNPALIISENQYSVEKKWLR
YVAHAISNEIFEKLTGIKGAFCTRIAYVLRIHNDHYPYELYISDYDGHNQISICRSTEPLMSPAWSPDGKKIAYVTFASG
HSELVIQALNTGLVNNIVSFPGHNGAPSFSPDCKKLAFSLSKTGSLNLYIMDLESGEITQLTKNRNNNTEPSWFPDNQNI
AYTSDQGGSPQIYKINVKTTEIQRLSWLHTSNQNPNVSSDGTFIIMVNRHQGKQNIAKLNLLTGQEEILTDTLLADSPSI
APNNTMVLYSNINKDLTTKSNLELISIDGHFKAHIQGDQGDIRFPTWSPLHLE

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=425, Percent_Identity=52.2352941176471, Blast_Score=484, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_BLOPB (Q492W9)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277848.1
- ProteinModelPortal:   Q492W9
- SMR:   Q492W9
- STRING:   Q492W9
- GeneID:   3562756
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_348
- eggNOG:   COG0823
- HOGENOM:   HBG644919
- OMA:   FCTRIAY
- ProtClustDB:   PRK03629
- BioCyc:   CBLO291272:BPEN_348-MONOMER
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 50849; Mature: 50849

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLIIKKTHKLPHWLQKVSLSIMLIIFLWKPALLADMHIEITCGVNAAHPIAVIPFTCVS
CEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHEEEEEEEEEECCCCCCCEEEEEEEECC
NQYNKSISIEDIASIIAADLRNSSKFNTIPVEYLPHKPTKVSDVIPTFWEKLGINIIVLG
CCCCCEECHHHHHHHHHHHHCCCCCCCEECEEECCCCCCCHHHHHHHHHHHHCCCEEEEE
TVHINYDESYIISYHLIDTSSNPALIISENQYSVEKKWLRYVAHAISNEIFEKLTGIKGA
EEEEECCCCEEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
FCTRIAYVLRIHNDHYPYELYISDYDGHNQISICRSTEPLMSPAWSPDGKKIAYVTFASG
HHHHHEEEEEECCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEECC
HSELVIQALNTGLVNNIVSFPGHNGAPSFSPDCKKLAFSLSKTGSLNLYIMDLESGEITQ
CCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCEEE
LTKNRNNNTEPSWFPDNQNIAYTSDQGGSPQIYKINVKTTEIQRLSWLHTSNQNPNVSSD
EECCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEEEHHHEEEEEEEECCCCCCCCCCC
GTFIIMVNRHQGKQNIAKLNLLTGQEEILTDTLLADSPSIAPNNTMVLYSNINKDLTTKS
CEEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCC
NLELISIDGHFKAHIQGDQGDIRFPTWSPLHLE
CEEEEEECCCEEEEEECCCCCEECCCCCCCCCC
>Mature Secondary Structure
MYLIIKKTHKLPHWLQKVSLSIMLIIFLWKPALLADMHIEITCGVNAAHPIAVIPFTCVS
CEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHEEEEEEEEEECCCCCCCEEEEEEEECC
NQYNKSISIEDIASIIAADLRNSSKFNTIPVEYLPHKPTKVSDVIPTFWEKLGINIIVLG
CCCCCEECHHHHHHHHHHHHCCCCCCCEECEEECCCCCCCHHHHHHHHHHHHCCCEEEEE
TVHINYDESYIISYHLIDTSSNPALIISENQYSVEKKWLRYVAHAISNEIFEKLTGIKGA
EEEEECCCCEEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
FCTRIAYVLRIHNDHYPYELYISDYDGHNQISICRSTEPLMSPAWSPDGKKIAYVTFASG
HHHHHEEEEEECCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEECC
HSELVIQALNTGLVNNIVSFPGHNGAPSFSPDCKKLAFSLSKTGSLNLYIMDLESGEITQ
CCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCEEE
LTKNRNNNTEPSWFPDNQNIAYTSDQGGSPQIYKINVKTTEIQRLSWLHTSNQNPNVSSD
EECCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEEEHHHEEEEEEEECCCCCCCCCCC
GTFIIMVNRHQGKQNIAKLNLLTGQEEILTDTLLADSPSIAPNNTMVLYSNINKDLTTKS
CEEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCC
NLELISIDGHFKAHIQGDQGDIRFPTWSPLHLE
CEEEEEECCCEEEEEECCCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA