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The map label for this gene is sucB [H]

Identifier: 71892110

GI number: 71892110

Start: 405720

End: 406964

Strand: Direct

Name: sucB [H]

Synonym: BPEN_342

Alternate gene names: 71892110

Gene position: 405720-406964 (Clockwise)

Preceding gene: 71892109

Following gene: 71892111

Centisome position: 51.25

GC content: 31.73

Gene sequence:

>1245_bases
ATGAGTAGTATTGACATTGTGGTTCCGAATTTACCAGAATCGGTTGCAGACGCTACTGTTGCTGTTTGGCATAAAAAATC
AGGAGATACAGTTAAACAAGATGATATTTTACTGGAAATAGAAACAGACAAAATTATGTTAGAAATTCCCGCTCCAAACA
CAGGAATTTTAGAATCAATTTCAGAACAAGAGGGATCAACAGTAGTATCCGGCCAAATTTTAGGCCGTCTAAATATTGAT
CATATCGTTTCCCAAAAAGATACAAAAAAAATATTTCAAGATCAAAAATCTATTACATCAGTAGCATCTCAAGAACATAT
TCTTACCCGAGATAACAAAAATCATAACATTTTAACTCCATCTATTCGCAAATTAATGACAGAGCATAATTTACAGCTAA
CAAATATTCAAGGCAGTGGAATAAAAGGACGTCTAACTCGTCAAGATATAGAGTCACATATTCATTCCGAAAAAAAATTA
CATTATACTAATCAACAAAATAATGAAAATGTAGCGCATCATCACATACAAAATACAAAAAACGATCGCAAAGAAACACG
TATTGTAATGAATCGATTACGAAAAAAAATCTCAGAACGCCTATTAGCTGTTACTAAAACTACAGCTATGTTAACTACTT
TTAATGAAGTAAACATGCAACCCATCATAAAACTGCGAAAAAAATATGGAGAATTATTTGAAAAACGTTATGGAATTACA
TTAGGAATCATGTCTTTTTATGTAAAAGCAGTTTTAGAAGGATTGAGACATTTTCCTGAAATTAATGCATCTATTGATGG
AGAAGAAATTGTATATTATCATTATTTTGATATAAGTATTGCAGTATCTACAGTACGTGGTTTAATTACTCCAGTGTTAC
GAGATATCGATACATTAAGCATGTCTGACATAGAAAAAAATATAAAATCTTTGGCTGAAAAAGGAAGAGACGGTAAACTA
ACAATTGAAGAATTAAGCGGAGGTAATTTTACCATTACTAACGGTGGAGTTTTTGGGTCATTAATGTCTACTCCTATCAT
TAATCCTCCACAAAGCGCAATTCTTGGTATGCATGCAATAAAGGACCGACCCATAGCTCAAGATGGGCAGATAGTAATAT
TACCTATGATGTATTTAGCATTATCATATGATCATAGATTAATAGATGGAAAAGATTCGGTAAGATTTCTAGTATATATC
AAAGAGTTGTTAGAAGACCCAACACGCTTGTTTTTAGAAATATAA

Upstream 100 bases:

>100_bases
CTGCACCTGCAGTAGGATATTTTTCAGTGCATCAACAACAACAAAAAAAATTAATTGACGATGCATTAAATCTAAATTAA
TTTTTTAAAAAGGATAGAAA

Downstream 100 bases:

>100_bases
TAACACAATATATATAAATATTTTTTATATTCTTATATTTTTAATAAATTAATACATTATATTAACTAAATTATTATCTT
AATCTTAAGCAATCACAATA

Product: dihydrolipoamide succinyltransferase E2 component

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MSSIDIVVPNLPESVADATVAVWHKKSGDTVKQDDILLEIETDKIMLEIPAPNTGILESISEQEGSTVVSGQILGRLNID
HIVSQKDTKKIFQDQKSITSVASQEHILTRDNKNHNILTPSIRKLMTEHNLQLTNIQGSGIKGRLTRQDIESHIHSEKKL
HYTNQQNNENVAHHHIQNTKNDRKETRIVMNRLRKKISERLLAVTKTTAMLTTFNEVNMQPIIKLRKKYGELFEKRYGIT
LGIMSFYVKAVLEGLRHFPEINASIDGEEIVYYHYFDISIAVSTVRGLITPVLRDIDTLSMSDIEKNIKSLAEKGRDGKL
TIEELSGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPIAQDGQIVILPMMYLALSYDHRLIDGKDSVRFLVYI
KELLEDPTRLFLEI

Sequences:

>Translated_414_residues
MSSIDIVVPNLPESVADATVAVWHKKSGDTVKQDDILLEIETDKIMLEIPAPNTGILESISEQEGSTVVSGQILGRLNID
HIVSQKDTKKIFQDQKSITSVASQEHILTRDNKNHNILTPSIRKLMTEHNLQLTNIQGSGIKGRLTRQDIESHIHSEKKL
HYTNQQNNENVAHHHIQNTKNDRKETRIVMNRLRKKISERLLAVTKTTAMLTTFNEVNMQPIIKLRKKYGELFEKRYGIT
LGIMSFYVKAVLEGLRHFPEINASIDGEEIVYYHYFDISIAVSTVRGLITPVLRDIDTLSMSDIEKNIKSLAEKGRDGKL
TIEELSGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPIAQDGQIVILPMMYLALSYDHRLIDGKDSVRFLVYI
KELLEDPTRLFLEI
>Mature_413_residues
SSIDIVVPNLPESVADATVAVWHKKSGDTVKQDDILLEIETDKIMLEIPAPNTGILESISEQEGSTVVSGQILGRLNIDH
IVSQKDTKKIFQDQKSITSVASQEHILTRDNKNHNILTPSIRKLMTEHNLQLTNIQGSGIKGRLTRQDIESHIHSEKKLH
YTNQQNNENVAHHHIQNTKNDRKETRIVMNRLRKKISERLLAVTKTTAMLTTFNEVNMQPIIKLRKKYGELFEKRYGITL
GIMSFYVKAVLEGLRHFPEINASIDGEEIVYYHYFDISIAVSTVRGLITPVLRDIDTLSMSDIEKNIKSLAEKGRDGKLT
IEELSGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPIAQDGQIVILPMMYLALSYDHRLIDGKDSVRFLVYIK
ELLEDPTRLFLEI

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=237, Percent_Identity=52.3206751054852, Blast_Score=259, Evalue=4e-69,
Organism=Homo sapiens, GI31711992, Length=439, Percent_Identity=28.4738041002278, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI110671329, Length=422, Percent_Identity=27.2511848341232, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI203098753, Length=451, Percent_Identity=26.6075388026608, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI203098816, Length=451, Percent_Identity=26.6075388026608, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI260898739, Length=168, Percent_Identity=35.7142857142857, Blast_Score=112, Evalue=8e-25,
Organism=Escherichia coli, GI1786946, Length=414, Percent_Identity=61.5942028985507, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI1786305, Length=404, Percent_Identity=26.7326732673267, Blast_Score=161, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI25146366, Length=232, Percent_Identity=49.5689655172414, Blast_Score=253, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI17560088, Length=442, Percent_Identity=29.6380090497738, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17537937, Length=433, Percent_Identity=23.094688221709, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17538894, Length=320, Percent_Identity=30, Blast_Score=138, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6320352, Length=410, Percent_Identity=37.0731707317073, Blast_Score=293, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6324258, Length=454, Percent_Identity=24.6696035242291, Blast_Score=133, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24645909, Length=226, Percent_Identity=53.9823008849557, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI18859875, Length=431, Percent_Identity=27.1461716937355, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI20129315, Length=228, Percent_Identity=32.0175438596491, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24582497, Length=228, Percent_Identity=32.0175438596491, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 46723; Mature: 46591

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIDIVVPNLPESVADATVAVWHKKSGDTVKQDDILLEIETDKIMLEIPAPNTGILESI
CCCCEEEECCCCHHHHCCEEEEEECCCCCCEECCCEEEEEECCEEEEEECCCCCHHHHHH
SEQEGSTVVSGQILGRLNIDHIVSQKDTKKIFQDQKSITSVASQEHILTRDNKNHNILTP
HHHCCCEEEEEHEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCEECH
SIRKLMTEHNLQLTNIQGSGIKGRLTRQDIESHIHSEKKLHYTNQQNNENVAHHHIQNTK
HHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHCCCHHEEECCCCCCCCHHHHHHHCCC
NDRKETRIVMNRLRKKISERLLAVTKTTAMLTTFNEVNMQPIIKLRKKYGELFEKRYGIT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHCCHHHHHHHHHHHHHHHHHHCCCH
LGIMSFYVKAVLEGLRHFPEINASIDGEEIVYYHYFDISIAVSTVRGLITPVLRDIDTLS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCC
MSDIEKNIKSLAEKGRDGKLTIEELSGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAI
HHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHH
KDRPIAQDGQIVILPMMYLALSYDHRLIDGKDSVRFLVYIKELLEDPTRLFLEI
CCCCCCCCCCEEEHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCHHHHEEEC
>Mature Secondary Structure 
SSIDIVVPNLPESVADATVAVWHKKSGDTVKQDDILLEIETDKIMLEIPAPNTGILESI
CCCEEEECCCCHHHHCCEEEEEECCCCCCEECCCEEEEEECCEEEEEECCCCCHHHHHH
SEQEGSTVVSGQILGRLNIDHIVSQKDTKKIFQDQKSITSVASQEHILTRDNKNHNILTP
HHHCCCEEEEEHEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCEECH
SIRKLMTEHNLQLTNIQGSGIKGRLTRQDIESHIHSEKKLHYTNQQNNENVAHHHIQNTK
HHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHCCCHHEEECCCCCCCCHHHHHHHCCC
NDRKETRIVMNRLRKKISERLLAVTKTTAMLTTFNEVNMQPIIKLRKKYGELFEKRYGIT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHCCHHHHHHHHHHHHHHHHHHCCCH
LGIMSFYVKAVLEGLRHFPEINASIDGEEIVYYHYFDISIAVSTVRGLITPVLRDIDTLS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCC
MSDIEKNIKSLAEKGRDGKLTIEELSGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAI
HHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHH
KDRPIAQDGQIVILPMMYLALSYDHRLIDGKDSVRFLVYIKELLEDPTRLFLEI
CCCCCCCCCCEEEHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCHHHHEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]