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The map label for this gene is def

Identifier: 71892002

GI number: 71892002

Start: 257858

End: 258379

Strand: Reverse

Name: def

Synonym: BPEN_226

Alternate gene names: 71892002

Gene position: 258379-257858 (Counterclockwise)

Preceding gene: 71892005

Following gene: 71892001

Centisome position: 32.64

GC content: 25.1

Gene sequence:

>522_bases
ATGTCGATATTAGAAATACTGTATTATCCAGATAAACGTCTTAGAACAATCGCTGATCCTGTTGTTGCGGTATCTGATGA
TACTAATCAAATTATAAATGATATGTTTGATACTATGTATTTTAAAAAAGGTATTGGTTTAGCTGCAACACAAGTTAATA
TTCACCAACAAATTATTGTTATTGATCTTTATAAAAAGAATAAACAACGTTTAGTTTTTATTAATCCTTCTATTACAAAA
AAAACAGGTATTATTAGTATTCCTGAAAGTTGTTTATCTATTCCTCAAATATACGAAATTGTTCCACGTTCAGAAAAAAT
AACCATTCAATCATTAGATCAATACGGCAATAAATTTGAAATGGAAGCTAATAATTTATTAGCAATTTGCATTCAACACG
AAGTAGATCATTTGTTTGGAAAACTTTTTATTGATCATTTGTCTCCATTAAAAATTAAAAAAATTCATAAGAAAATAAAA
AAATTATCTAAAACATTTAAAAAGAATCAATTTCCTCTTTGA

Upstream 100 bases:

>100_bases
TCATTATGAATATAAAATATCTATATCATATCTTGATTATATTCGTAAGTCACTAGATAATATATGGAATTAAAAATTTA
ATGTAATAATTTTTTATTTT

Downstream 100 bases:

>100_bases
TATATATATTATGGTACACTCAAAATCATTGCGTATTGCTTTTTTTGGAACTACAAGTTTTGCAGCGTGGCATTTACATA
CACTGGCTCACTTATCTACG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MSILEILYYPDKRLRTIADPVVAVSDDTNQIINDMFDTMYFKKGIGLAATQVNIHQQIIVIDLYKKNKQRLVFINPSITK
KTGIISIPESCLSIPQIYEIVPRSEKITIQSLDQYGNKFEMEANNLLAICIQHEVDHLFGKLFIDHLSPLKIKKIHKKIK
KLSKTFKKNQFPL

Sequences:

>Translated_173_residues
MSILEILYYPDKRLRTIADPVVAVSDDTNQIINDMFDTMYFKKGIGLAATQVNIHQQIIVIDLYKKNKQRLVFINPSITK
KTGIISIPESCLSIPQIYEIVPRSEKITIQSLDQYGNKFEMEANNLLAICIQHEVDHLFGKLFIDHLSPLKIKKIHKKIK
KLSKTFKKNQFPL
>Mature_172_residues
SILEILYYPDKRLRTIADPVVAVSDDTNQIINDMFDTMYFKKGIGLAATQVNIHQQIIVIDLYKKNKQRLVFINPSITKK
TGIISIPESCLSIPQIYEIVPRSEKITIQSLDQYGNKFEMEANNLLAICIQHEVDHLFGKLFIDHLSPLKIKKIHKKIKK
LSKTFKKNQFPL

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=157, Percent_Identity=28.6624203821656, Blast_Score=77, Evalue=9e-15,
Organism=Escherichia coli, GI1789682, Length=164, Percent_Identity=55.4878048780488, Blast_Score=204, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24645728, Length=166, Percent_Identity=28.3132530120482, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_BLOPB (Q493I1)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277732.1
- HSSP:   P0A6K3
- ProteinModelPortal:   Q493I1
- SMR:   Q493I1
- STRING:   Q493I1
- GeneID:   3562861
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_226
- eggNOG:   COG0242
- HOGENOM:   HBG665227
- OMA:   MVIKKFT
- ProtClustDB:   PRK00150
- BioCyc:   CBLO291272:BPEN_226-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 19985; Mature: 19854

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: ACT_SITE 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILEILYYPDKRLRTIADPVVAVSDDTNQIINDMFDTMYFKKGIGLAATQVNIHQQIIV
CCCEEEEECCCHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEE
IDLYKKNKQRLVFINPSITKKTGIISIPESCLSIPQIYEIVPRSEKITIQSLDQYGNKFE
EEEEECCCCEEEEECCCCCCCCCEEECCHHHCCHHHHHHHCCCCCEEHHEEHHHCCCEEE
MEANNLLAICIQHEVDHLFGKLFIDHLSPLKIKKIHKKIKKLSKTFKKNQFPL
ECCCCEEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SILEILYYPDKRLRTIADPVVAVSDDTNQIINDMFDTMYFKKGIGLAATQVNIHQQIIV
CCEEEEECCCHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEE
IDLYKKNKQRLVFINPSITKKTGIISIPESCLSIPQIYEIVPRSEKITIQSLDQYGNKFE
EEEEECCCCEEEEECCCCCCCCCEEECCHHHCCHHHHHHHCCCCCEEHHEEHHHCCCEEE
MEANNLLAICIQHEVDHLFGKLFIDHLSPLKIKKIHKKIKKLSKTFKKNQFPL
ECCCCEEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA