Click here to switch to the map view.

The map label for this gene is gloB

Identifier: 71892005

GI number: 71892005

Start: 260930

End: 261685

Strand: Reverse

Name: gloB

Synonym: BPEN_230

Alternate gene names: 71892005

Gene position: 261685-260930 (Counterclockwise)

Preceding gene: 71892006

Following gene: 71892002

Centisome position: 33.06

GC content: 30.03

Gene sequence:

>756_bases
ATGAATATCATTAGAGTCCCGGTATTAAGCACTAATTATATTTGGTGTTTGTATAATTATAAAAATGAATGCATAATTAT
TGATCCCGGAGAAGCAACAAAGGTACTAGATATTTTAAAAAAATTTCAATTTAGATTACGAGCAATTTTATTGACTCATA
ATCATATTGATCATGTCAATGGAGTAGCCCCTTTAATTCAATATTTTCCAAAAACAATTGTTTATGGTCCTACGGAAACT
AAAAATAATGGCTCTCATTTTTTGGTATCAGAAGGAGATGATTTTGTATTATTACAAAAAAAATTTAAAGTTCTCAATTT
ACCTGGGCATACACCGGGGCATATCGGGTTTTATAGCGCACCCTGGTTATTTTGCGGAGATACTGTATTTTCAGCAGGTT
GTGGTAAAATCTGTATAGGATTCGCTCAATATATGTATGAATCCTTTTTAAAAATTAGATATCTTCCTCGTAATACCTTA
ATTTTCAGCGGCCATGAATATACTCTATCTAATGTTAACTTCGCTATTTCTATTTTACCTCAAGATCAATCCATTATCAA
CTATCGCAATAAGATTATTAAATTATATAAAAAAAAACAACCCACTGTGCCAACAACACTAAATTTAGAACTAAAGGTTA
ATCCTTTCTTTCGTTGTGGTAACTCAAATATAAAAAAATCATTAAATTTACCCTGTGACCTTAAAGAAGAGTGGCAAGTT
TTTTCTGAATTACGTAAGAAAAAAGATTCATTTTAG

Upstream 100 bases:

>100_bases
AACAATTAATCTAAGTACTAAAAATAATATTTTTACCCATTGAATATTTCTGAAAAAATTTTAAAATACTTATTTTAAAT
TTGTATCACTACGATCCATG

Downstream 100 bases:

>100_bases
TCTTTATTATAAAGTATAATTACCGAATTTCAGTCATTACCTTCTATTATAAATTTTTTATTTTAACAATTAATTATTAC
ATTTCTAATTAAAACTTTTT

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MNIIRVPVLSTNYIWCLYNYKNECIIIDPGEATKVLDILKKFQFRLRAILLTHNHIDHVNGVAPLIQYFPKTIVYGPTET
KNNGSHFLVSEGDDFVLLQKKFKVLNLPGHTPGHIGFYSAPWLFCGDTVFSAGCGKICIGFAQYMYESFLKIRYLPRNTL
IFSGHEYTLSNVNFAISILPQDQSIINYRNKIIKLYKKKQPTVPTTLNLELKVNPFFRCGNSNIKKSLNLPCDLKEEWQV
FSELRKKKDSF

Sequences:

>Translated_251_residues
MNIIRVPVLSTNYIWCLYNYKNECIIIDPGEATKVLDILKKFQFRLRAILLTHNHIDHVNGVAPLIQYFPKTIVYGPTET
KNNGSHFLVSEGDDFVLLQKKFKVLNLPGHTPGHIGFYSAPWLFCGDTVFSAGCGKICIGFAQYMYESFLKIRYLPRNTL
IFSGHEYTLSNVNFAISILPQDQSIINYRNKIIKLYKKKQPTVPTTLNLELKVNPFFRCGNSNIKKSLNLPCDLKEEWQV
FSELRKKKDSF
>Mature_251_residues
MNIIRVPVLSTNYIWCLYNYKNECIIIDPGEATKVLDILKKFQFRLRAILLTHNHIDHVNGVAPLIQYFPKTIVYGPTET
KNNGSHFLVSEGDDFVLLQKKFKVLNLPGHTPGHIGFYSAPWLFCGDTVFSAGCGKICIGFAQYMYESFLKIRYLPRNTL
IFSGHEYTLSNVNFAISILPQDQSIINYRNKIIKLYKKKQPTVPTTLNLELKVNPFFRCGNSNIKKSLNLPCDLKEEWQV
FSELRKKKDSF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI14150041, Length=246, Percent_Identity=31.7073170731707, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI94538320, Length=260, Percent_Identity=30.3846153846154, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI94538322, Length=260, Percent_Identity=30.3846153846154, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI46361987, Length=193, Percent_Identity=32.1243523316062, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI21703352, Length=263, Percent_Identity=29.277566539924, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI116642887, Length=263, Percent_Identity=29.277566539924, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1786406, Length=251, Percent_Identity=43.4262948207171, Blast_Score=213, Evalue=9e-57,
Organism=Escherichia coli, GI1787158, Length=158, Percent_Identity=30.379746835443, Blast_Score=68, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17536925, Length=222, Percent_Identity=30.6306306306306, Blast_Score=97, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6320478, Length=263, Percent_Identity=26.9961977186312, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24667711, Length=262, Percent_Identity=33.206106870229, Blast_Score=136, Evalue=1e-32,
Organism=Drosophila melanogaster, GI21356335, Length=262, Percent_Identity=33.206106870229, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24667703, Length=262, Percent_Identity=33.206106870229, Blast_Score=135, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_BLOPB (Q493H8)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277735.1
- ProteinModelPortal:   Q493H8
- SMR:   Q493H8
- STRING:   Q493H8
- GeneID:   3562806
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_230
- eggNOG:   COG0491
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- ProtClustDB:   CLSK280747
- BioCyc:   CBLO291272:BPEN_230-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 28934; Mature: 28934

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIIRVPVLSTNYIWCLYNYKNECIIIDPGEATKVLDILKKFQFRLRAILLTHNHIDHVN
CCEEEEEEECCCEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHEEECCCCHHHH
GVAPLIQYFPKTIVYGPTETKNNGSHFLVSEGDDFVLLQKKFKVLNLPGHTPGHIGFYSA
HHHHHHHHCCHHEEECCCCCCCCCCEEEEECCCCEEEEEECEEEEECCCCCCCCCCCCCC
PWLFCGDTVFSAGCGKICIGFAQYMYESFLKIRYLPRNTLIFSGHEYTLSNVNFAISILP
CEEEECCHHHHCCCCHHHHHHHHHHHHHHHEEEECCCCEEEECCCEEEECCCEEEEEEEC
QDQSIINYRNKIIKLYKKKQPTVPTTLNLELKVNPFFRCGNSNIKKSLNLPCDLKEEWQV
CCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCEEEECCCCCHHCCCCCCCCHHHHHH
FSELRKKKDSF
HHHHHHHCCCC
>Mature Secondary Structure
MNIIRVPVLSTNYIWCLYNYKNECIIIDPGEATKVLDILKKFQFRLRAILLTHNHIDHVN
CCEEEEEEECCCEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHEEECCCCHHHH
GVAPLIQYFPKTIVYGPTETKNNGSHFLVSEGDDFVLLQKKFKVLNLPGHTPGHIGFYSA
HHHHHHHHCCHHEEECCCCCCCCCCEEEEECCCCEEEEEECEEEEECCCCCCCCCCCCCC
PWLFCGDTVFSAGCGKICIGFAQYMYESFLKIRYLPRNTLIFSGHEYTLSNVNFAISILP
CEEEECCHHHHCCCCHHHHHHHHHHHHHHHEEEECCCCEEEECCCEEEECCCEEEEEEEC
QDQSIINYRNKIIKLYKKKQPTVPTTLNLELKVNPFFRCGNSNIKKSLNLPCDLKEEWQV
CCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCEEEECCCCCHHCCCCCCCCHHHHHH
FSELRKKKDSF
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA