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The map label for this gene is yciL [C]

Identifier: 68535954

GI number: 68535954

Start: 1046886

End: 1047758

Strand: Direct

Name: yciL [C]

Synonym: jk0877

Alternate gene names: 68535954

Gene position: 1046886-1047758 (Clockwise)

Preceding gene: 68535953

Following gene: 68535955

Centisome position: 42.51

GC content: 63.8

Gene sequence:

>873_bases
GTGGACACACCCGCTCGCCGAGACGGCACACCGGAGACCGCTCCGAAAGACATCTACCTATCCCAGGCCAAGCCCGCCAA
GCGTCAGCACGTAACGGCGCAGGAGGCGGAGGCTGCTCTCAATAAGCCAGAATCGGAGAAAGCGCAGGTGCGCCTGCAAA
AGGTGCTGGCCTCCGCGGGTGTGGCTAGCCGCCGCATGAGCGAGAAGCTGATCGCCGCCGGTCGCGTGGAAGTCAACGGC
GAAGTCGTGGTGAAGATGGGCACCCGCATCGACCCGAGCGTCGACATCGTCCGCGTGGATGGCGAGCGCGTGCAGGTCAA
CGAAGAGATGGTCTACTTCGTTCTGAACAAGCCCCGCGGCGTGCACTCCACGATGAGCGACGACCTGGGCCGCCCGTGCG
TGGGAGACCTGGTTGGTACGCGCCTTAAGGAAGGCCAGGGACTATTCCACGTCGGCCGTCTGGATGCCCAGACCGAAGGT
CTGCTGCTGCTCACGAACGACGGTGAACTCGCCAACCGCCTGATGCACCCCAAGTACGAGGTGAAGAAGACCTACATGGC
CACGGTGCTGGGAGAGGCGAGCCGCAAGCTGATCCGCGAGCTGAAGGACGGCGTGGAGCTGGACGATGGCATCGCGCGTG
CCGACAGCGTGCAGATCGTGGACGTGTGGCAGGGCAAGTCGCTGATCAAGGTGGAGCTGCATGAGGGACGCAAGCACATC
GTGCGCCGCATGCTGAAGGAAGCCGGCTTCCCGGTGCAGGCTCTGGTGCGCACAAAGATCCACACCGTTCAGCTGGGCGA
GCAGAAGCCGGGCACGATTCGCGCACTGAACCAGGCGGAACTGCAGAGCATGTACAAGAAGGTCGGGCTGTAG

Upstream 100 bases:

>100_bases
GACAGTATCGACAGCCTTGACAATATCGACAATTAAGCAGGTTGAAGGGTATAGTTCGGGCTGATAATTCAAGATTTTAG
TTCGAAGGGCTAGAAACACC

Downstream 100 bases:

>100_bases
CTAGATCAGCCGACGTAAACACACAATCAATTAGGGGAACGACCTTTTTATGACGAACGCACCTTTCTTCGAGCCAGGCT
CCGCCCTGGATCAGCTCGCC

Product: putative pseudouridylate synthase

Products: NA

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MDTPARRDGTPETAPKDIYLSQAKPAKRQHVTAQEAEAALNKPESEKAQVRLQKVLASAGVASRRMSEKLIAAGRVEVNG
EVVVKMGTRIDPSVDIVRVDGERVQVNEEMVYFVLNKPRGVHSTMSDDLGRPCVGDLVGTRLKEGQGLFHVGRLDAQTEG
LLLLTNDGELANRLMHPKYEVKKTYMATVLGEASRKLIRELKDGVELDDGIARADSVQIVDVWQGKSLIKVELHEGRKHI
VRRMLKEAGFPVQALVRTKIHTVQLGEQKPGTIRALNQAELQSMYKKVGL

Sequences:

>Translated_290_residues
MDTPARRDGTPETAPKDIYLSQAKPAKRQHVTAQEAEAALNKPESEKAQVRLQKVLASAGVASRRMSEKLIAAGRVEVNG
EVVVKMGTRIDPSVDIVRVDGERVQVNEEMVYFVLNKPRGVHSTMSDDLGRPCVGDLVGTRLKEGQGLFHVGRLDAQTEG
LLLLTNDGELANRLMHPKYEVKKTYMATVLGEASRKLIRELKDGVELDDGIARADSVQIVDVWQGKSLIKVELHEGRKHI
VRRMLKEAGFPVQALVRTKIHTVQLGEQKPGTIRALNQAELQSMYKKVGL
>Mature_290_residues
MDTPARRDGTPETAPKDIYLSQAKPAKRQHVTAQEAEAALNKPESEKAQVRLQKVLASAGVASRRMSEKLIAAGRVEVNG
EVVVKMGTRIDPSVDIVRVDGERVQVNEEMVYFVLNKPRGVHSTMSDDLGRPCVGDLVGTRLKEGQGLFHVGRLDAQTEG
LLLLTNDGELANRLMHPKYEVKKTYMATVLGEASRKLIRELKDGVELDDGIARADSVQIVDVWQGKSLIKVELHEGRKHI
VRRMLKEAGFPVQALVRTKIHTVQLGEQKPGTIRALNQAELQSMYKKVGL

Specific function: Unknown

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=225, Percent_Identity=40.4444444444444, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1790453, Length=243, Percent_Identity=30.0411522633745, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788510, Length=238, Percent_Identity=28.9915966386555, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87081838, Length=166, Percent_Identity=32.5301204819277, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 32081; Mature: 32081

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTPARRDGTPETAPKDIYLSQAKPAKRQHVTAQEAEAALNKPESEKAQVRLQKVLASAG
CCCCCCCCCCCCCCCCCEEECCCCCHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHCC
VASRRMSEKLIAAGRVEVNGEVVVKMGTRIDPSVDIVRVDGERVQVNEEMVYFVLNKPRG
HHHHHHHHHHHHCCEEEECCEEEEEECCCCCCCEEEEEECCCEEEECCEEEEEEECCCCC
VHSTMSDDLGRPCVGDLVGTRLKEGQGLFHVGRLDAQTEGLLLLTNDGELANRLMHPKYE
CCHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCHHHHHHHCCCCHH
VKKTYMATVLGEASRKLIRELKDGVELDDGIARADSVQIVDVWQGKSLIKVELHEGRKHI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCCHHHH
VRRMLKEAGFPVQALVRTKIHTVQLGEQKPGTIRALNQAELQSMYKKVGL
HHHHHHHCCCCHHHHHHHHHHEEEECCCCCCCEECCCHHHHHHHHHHHCC
>Mature Secondary Structure
MDTPARRDGTPETAPKDIYLSQAKPAKRQHVTAQEAEAALNKPESEKAQVRLQKVLASAG
CCCCCCCCCCCCCCCCCEEECCCCCHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHCC
VASRRMSEKLIAAGRVEVNGEVVVKMGTRIDPSVDIVRVDGERVQVNEEMVYFVLNKPRG
HHHHHHHHHHHHCCEEEECCEEEEEECCCCCCCEEEEEECCCEEEECCEEEEEEECCCCC
VHSTMSDDLGRPCVGDLVGTRLKEGQGLFHVGRLDAQTEGLLLLTNDGELANRLMHPKYE
CCHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCHHHHHHHCCCCHH
VKKTYMATVLGEASRKLIRELKDGVELDDGIARADSVQIVDVWQGKSLIKVELHEGRKHI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCCHHHH
VRRMLKEAGFPVQALVRTKIHTVQLGEQKPGTIRALNQAELQSMYKKVGL
HHHHHHHCCCCHHHHHHHHHHEEEECCCCCCCEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]