Click here to switch to the map view.

The map label for this gene is ysgA [H]

Identifier: 68535913

GI number: 68535913

Start: 997665

End: 998522

Strand: Direct

Name: ysgA [H]

Synonym: jk0838

Alternate gene names: 68535913

Gene position: 997665-998522 (Clockwise)

Preceding gene: 68535912

Following gene: 68535914

Centisome position: 40.51

GC content: 63.87

Gene sequence:

>858_bases
ATGCTGTGGGGTATGAACCCGCAGGATGCCCAAGAGCTTTTCACCGAAAGAACCCCTCGCATTGTCAACGCAGCCAAGCT
TCACCGGGCGGCCGCGCGCAAGAAGGCGGGGGTTTTCCTTGTGGAGGGGGAGAATAGCGTAGAAGCCGCCATCGCCACCG
GAAGTGCCACGGACGTGTACGTCACGCTGGCCGCCGCGGAGCGCTTCGAGCCGATCATCCGCACCGCCGGCTACCTCAAT
ATCTACGTGCACTACATCACGGACAAGGCGGCCAAGCACCTTTCTGATACCGCCACGACTACAGGTTTGTTTGCCGTGTG
CAAGCCGGTGCTGTGGTCTGTCGGTAAGGTATTGACGCCCCAGCCTCGGCTCGTGAGCGTCCCAGTGGAAACCAACGACC
CCGGCAATGCAGGCACCCTGATTCGAGTGGCTGATGCCATGGGCGCGGATGCCGTGGTGTTTGTTGGGGAGACTGCTGAC
CCGCAGTCGTGCAAGGCCGCTCGCGCTTCGGCGGGCTCACTGTTCCACGTGCCGGTGGCCCGCTCCACCAATGTGAAGGA
TGTGTTGGGCCAGCTGCGTGCCCAGGGGATGCAGATTCTCGCCACTGCGGCCGATGGTGAGGTCAGCCTGGATGATGCGG
GGGAGATCCTCACCAAGCCCACCGCGTGGCTGTTCGGCAATGAGGCGCACGGGCTGGGCGAGGAGCTTCTGGCCGAGGCG
GACCTGCGGGTTTCCATCCCGATCCGTGGCCGTGCGGAATCCCTCAACCTTGCCACCGCGGCCTCCATCTGCATGTATGA
ATCGTCCCGCATCCAGGCGGCCGCACGTGAGGCTGCCGGGGGAGCAGTCGAAGACTGA

Upstream 100 bases:

>100_bases
CAGGAGGCCAAGGATGGGACGATGACGCGCACTCATTCCAAGAACGGCGTTACCACTACGGAGAAGGAGCAGATGACCCT
GTTCGAAAACTAGGTCAATT

Downstream 100 bases:

>100_bases
TAGCCCCAACTCGGTGCGGCAGTGGGCCAGCCGGGTATTATTAACGAGGTTAAGCCGTGCACGTAGATAGAGGAGCGCAC
TGAAGTGACGGATTCCGCGG

Product: putative rRNA methylase

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MLWGMNPQDAQELFTERTPRIVNAAKLHRAAARKKAGVFLVEGENSVEAAIATGSATDVYVTLAAAERFEPIIRTAGYLN
IYVHYITDKAAKHLSDTATTTGLFAVCKPVLWSVGKVLTPQPRLVSVPVETNDPGNAGTLIRVADAMGADAVVFVGETAD
PQSCKAARASAGSLFHVPVARSTNVKDVLGQLRAQGMQILATAADGEVSLDDAGEILTKPTAWLFGNEAHGLGEELLAEA
DLRVSIPIRGRAESLNLATAASICMYESSRIQAAAREAAGGAVED

Sequences:

>Translated_285_residues
MLWGMNPQDAQELFTERTPRIVNAAKLHRAAARKKAGVFLVEGENSVEAAIATGSATDVYVTLAAAERFEPIIRTAGYLN
IYVHYITDKAAKHLSDTATTTGLFAVCKPVLWSVGKVLTPQPRLVSVPVETNDPGNAGTLIRVADAMGADAVVFVGETAD
PQSCKAARASAGSLFHVPVARSTNVKDVLGQLRAQGMQILATAADGEVSLDDAGEILTKPTAWLFGNEAHGLGEELLAEA
DLRVSIPIRGRAESLNLATAASICMYESSRIQAAAREAAGGAVED
>Mature_285_residues
MLWGMNPQDAQELFTERTPRIVNAAKLHRAAARKKAGVFLVEGENSVEAAIATGSATDVYVTLAAAERFEPIIRTAGYLN
IYVHYITDKAAKHLSDTATTTGLFAVCKPVLWSVGKVLTPQPRLVSVPVETNDPGNAGTLIRVADAMGADAVVFVGETAD
PQSCKAARASAGSLFHVPVARSTNVKDVLGQLRAQGMQILATAADGEVSLDDAGEILTKPTAWLFGNEAHGLGEELLAEA
DLRVSIPIRGRAESLNLATAASICMYESSRIQAAAREAAGGAVED

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=152, Percent_Identity=36.1842105263158, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790623, Length=210, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 29981; Mature: 29981

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLWGMNPQDAQELFTERTPRIVNAAKLHRAAARKKAGVFLVEGENSVEAAIATGSATDVY
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEECCCCEEEEEECCCCCEEE
VTLAAAERFEPIIRTAGYLNIYVHYITDKAAKHLSDTATTTGLFAVCKPVLWSVGKVLTP
EEEECHHHHHHHHHHCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QPRLVSVPVETNDPGNAGTLIRVADAMGADAVVFVGETADPQSCKAARASAGSLFHVPVA
CCCEEEEEECCCCCCCCCCEEEEECCCCCCEEEEEECCCCCHHHHHHHCCCCCEEEECCC
RSTNVKDVLGQLRAQGMQILATAADGEVSLDDAGEILTKPTAWLFGNEAHGLGEELLAEA
CCCCHHHHHHHHHHCCEEEEEEECCCCEECCCCCHHHCCCCEEEECCCCCCCHHHHHHCC
DLRVSIPIRGRAESLNLATAASICMYESSRIQAAAREAAGGAVED
CCEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLWGMNPQDAQELFTERTPRIVNAAKLHRAAARKKAGVFLVEGENSVEAAIATGSATDVY
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEECCCCEEEEEECCCCCEEE
VTLAAAERFEPIIRTAGYLNIYVHYITDKAAKHLSDTATTTGLFAVCKPVLWSVGKVLTP
EEEECHHHHHHHHHHCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QPRLVSVPVETNDPGNAGTLIRVADAMGADAVVFVGETADPQSCKAARASAGSLFHVPVA
CCCEEEEEECCCCCCCCCCEEEEECCCCCCEEEEEECCCCCHHHHHHHCCCCCEEEECCC
RSTNVKDVLGQLRAQGMQILATAADGEVSLDDAGEILTKPTAWLFGNEAHGLGEELLAEA
CCCCHHHHHHHHHHCCEEEEEEECCCCEECCCCCHHHCCCCEEEECCCCCCCHHHHHHCC
DLRVSIPIRGRAESLNLATAASICMYESSRIQAAAREAAGGAVED
CCEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]