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The map label for this gene is hisF

Identifier: 68535868

GI number: 68535868

Start: 946896

End: 947678

Strand: Direct

Name: hisF

Synonym: jk0793

Alternate gene names: 68535868

Gene position: 946896-947678 (Clockwise)

Preceding gene: 68535867

Following gene: 68535869

Centisome position: 38.45

GC content: 67.94

Gene sequence:

>783_bases
ATGAGTGTTACCGTGCGCGTCATTCCGTGCCTGGATGTGGACAACGGCCGCGTTGTCAAGGGCGTGAACTTCGAGGGCCT
GCGCGATGCAGGCGACCCCGTCGAGCTGGCCAAGCGCTACGACGCGCTGGGCGCCGACGAGCTGACCTTCCTGGACGTCT
CCGCCTCCAAGGACGGGCGCGGCACCATGCTGGATGTTGTGCGCCACACCGCCGACCAGGTGTTTATCCCGTTAACCGTC
GGCGGGGGAGTGCGCAGCGAGGAGGACGTGGACGCCCTGCTGCGCGCCGGTGCGGACAAGGTCAGCGTCAATTCCTCGGC
AGTCGCCCGCCCCGAGCTGCTGCGCGAACTTTCCCAGCGCTTCGGTGCGCAATGCATCGTCCTGTCCGTGGACGCGCGCC
GCGTAAAGGATCCCGTGGAGGCGGACAAGTACCCCTCCGGCTTTGAGGTCACCACCCATGGCGGCACCAAGTCCGCCGGG
CTGGATGCCATCGAGTGGGCCCGCAAGGGCGAGGAGCTGGGCGTAGGGGAGATCCTGCTGAACTCCATGGACGGCGACGG
CACCAAGGCCGGCTTCGACATCGAGATGCTGCAGGCCGTGCGCAAGGCCGTCTCCATTCCCGTCATTGCCTCCGGCGGCG
CGGGCGCCCCGGATCACTTCCCGCCCGCCGTGGAGGCTGGCGCGGATGCCGTGCTGGCGGCCTCCATCTTCCACTTCGGC
GAGGTCGAGATCCGCGAGGTCAAGCAGGCTCTGGCCGATGCGGGCTACGAGGTACGACTGTGA

Upstream 100 bases:

>100_bases
AGCCGGAGGTGCACGCCACGCTCCTGCAGCACATTCAGGCCGTGCCGATTGGTACTGCCGCCCGCAACGCCCAGGAGATT
CGCGACCGAGGAGGCATTCG

Downstream 100 bases:

>100_bases
GCAACACAGACTCCAAGACAGGCCCCAACACAGACCCGGCAACCTGCGAGCTAGACCCCGCCATCGCCGAACGCGTCCGC
TTCAACGCTGACGGCTTAGT

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MSVTVRVIPCLDVDNGRVVKGVNFEGLRDAGDPVELAKRYDALGADELTFLDVSASKDGRGTMLDVVRHTADQVFIPLTV
GGGVRSEEDVDALLRAGADKVSVNSSAVARPELLRELSQRFGAQCIVLSVDARRVKDPVEADKYPSGFEVTTHGGTKSAG
LDAIEWARKGEELGVGEILLNSMDGDGTKAGFDIEMLQAVRKAVSIPVIASGGAGAPDHFPPAVEAGADAVLAASIFHFG
EVEIREVKQALADAGYEVRL

Sequences:

>Translated_260_residues
MSVTVRVIPCLDVDNGRVVKGVNFEGLRDAGDPVELAKRYDALGADELTFLDVSASKDGRGTMLDVVRHTADQVFIPLTV
GGGVRSEEDVDALLRAGADKVSVNSSAVARPELLRELSQRFGAQCIVLSVDARRVKDPVEADKYPSGFEVTTHGGTKSAG
LDAIEWARKGEELGVGEILLNSMDGDGTKAGFDIEMLQAVRKAVSIPVIASGGAGAPDHFPPAVEAGADAVLAASIFHFG
EVEIREVKQALADAGYEVRL
>Mature_259_residues
SVTVRVIPCLDVDNGRVVKGVNFEGLRDAGDPVELAKRYDALGADELTFLDVSASKDGRGTMLDVVRHTADQVFIPLTVG
GGVRSEEDVDALLRAGADKVSVNSSAVARPELLRELSQRFGAQCIVLSVDARRVKDPVEADKYPSGFEVTTHGGTKSAGL
DAIEWARKGEELGVGEILLNSMDGDGTKAGFDIEMLQAVRKAVSIPVIASGGAGAPDHFPPAVEAGADAVLAASIFHFGE
VEIREVKQALADAGYEVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=43.75, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI87082028, Length=244, Percent_Identity=29.5081967213115, Blast_Score=93, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=39.171974522293, Blast_Score=193, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_CORJK (Q4JW52)

Other databases:

- EMBL:   CR931997
- RefSeq:   YP_250573.1
- ProteinModelPortal:   Q4JW52
- SMR:   Q4JW52
- STRING:   Q4JW52
- GeneID:   3433181
- GenomeReviews:   CR931997_GR
- KEGG:   cjk:jk0793
- NMPDR:   fig|306537.3.peg.1766
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- BioCyc:   CJEI306537:JK0793-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27413; Mature: 27282

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: ACT_SITE 12-12 ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTVRVIPCLDVDNGRVVKGVNFEGLRDAGDPVELAKRYDALGADELTFLDVSASKDGR
CCEEEEEEEEEECCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCC
GTMLDVVRHTADQVFIPLTVGGGVRSEEDVDALLRAGADKVSVNSSAVARPELLRELSQR
CHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHH
FGAQCIVLSVDARRVKDPVEADKYPSGFEVTTHGGTKSAGLDAIEWARKGEELGVGEILL
HCCEEEEEECCHHHHCCCHHHCCCCCCCEEEECCCCCCCCCHHHHHHHCCCCCCHHHHHH
NSMDGDGTKAGFDIEMLQAVRKAVSIPVIASGGAGAPDHFPPAVEAGADAVLAASIFHFG
CCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHCCHHHHHHHHHHCC
EVEIREVKQALADAGYEVRL
CHHHHHHHHHHHCCCCEECC
>Mature Secondary Structure 
SVTVRVIPCLDVDNGRVVKGVNFEGLRDAGDPVELAKRYDALGADELTFLDVSASKDGR
CEEEEEEEEEECCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCC
GTMLDVVRHTADQVFIPLTVGGGVRSEEDVDALLRAGADKVSVNSSAVARPELLRELSQR
CHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHH
FGAQCIVLSVDARRVKDPVEADKYPSGFEVTTHGGTKSAGLDAIEWARKGEELGVGEILL
HCCEEEEEECCHHHHCCCHHHCCCCCCCEEEECCCCCCCCCHHHHHHHCCCCCCHHHHHH
NSMDGDGTKAGFDIEMLQAVRKAVSIPVIASGGAGAPDHFPPAVEAGADAVLAASIFHFG
CCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHCCHHHHHHHHHHCC
EVEIREVKQALADAGYEVRL
CHHHHHHHHHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA