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The map label for this gene is glk [H]

Identifier: 68535803

GI number: 68535803

Start: 868495

End: 869463

Strand: Direct

Name: glk [H]

Synonym: jk0728

Alternate gene names: 68535803

Gene position: 868495-869463 (Clockwise)

Preceding gene: 68535802

Following gene: 68535804

Centisome position: 35.27

GC content: 65.33

Gene sequence:

>969_bases
GTGACTGGGACCCTAACCGTGGGCGTGGACATCGGTGGCACGAACCTGCGCGCCGCCGTAGTAGACGCGGACGGGCAGAT
CCTGGACATCGAGAAACTACCCACGCCCTCTAACGTGTACGCGCTGGAGACCGCTTTGGTGCACGCCATCAACTGCCTAC
AGCGCCGCAACGAAGGTATTGTGGCGGTTGGTCTGGCCGTGGCGGGGTTCATTAACTCCCAGCGCGATACCGTGCGCTTC
GCCCCGCACCTGCCGTGGCGCAACGCACAGGTGACCGAGCGCCTGAGTGCCAAGTTGGGGCTGCCGGTGGAGCTGGAGCA
CGACGCAAACTCGGCAGCGTTGGGGGAGTACTACCTGGGAGCCGCACAGAACGCGGGGACTTGGGTTCTGTTCGCGCTGG
GTACTGGCATCGGTGGCGCCGTGATGGTGAATGGGGAGATCTACCGCGGAGCCTTCGGCACCGCCCCGGAGTTTGGGCAC
CTGACTGTAATGCCGGGCGGACGGGCGTGCCCCTGTGGCAAGCAGGGCTGTCTGGAGCGCTACTGCTCCGGCTCGGCGCT
GGCGCTGACGGCGCAGGACCTGATTGCAACCGGTCGCTACCCGGATTCGGCCATCACCCGCGAGTTTGCGCAGCACCCGG
AGGAGATCTCCGGCCGGACGATCGTGCGCCTAGCTAGGGAGGGCGATCCACTGGCCCGCGCGGCCATTGAGGACATGGGC
CTGTGGCTGGGGCGTGGGCTGTCGCTGGTGCAGGACGTGTTTGATCCGGAGTTGATTGTGATAGGCGGGGGAGTTAGCTC
CGACGCGGATCTGTTCTTGGACCAAGCGGAATCCATGATGAACTACAGCATCGTCGGCGCTGGCCACCGCCCGGTGGCAG
AAGTGAAGACCGCGCAGTTGGGCGGCGATGCCGGTATGATCGGCGTGGCGCTGCTGGCACGCAAGCATCTTGAGCGTGGC
GCTGAATAA

Upstream 100 bases:

>100_bases
TTCTATGTGCTGCCAGGCTGGCTAGAATGGTGGGGTTAATCGGTACAGACGAGTATCGGTACAGGCAAGTACAGAAGAAG
TAGAGAGGGCGGGACGCCTA

Downstream 100 bases:

>100_bases
GGAAGCCATCGAGGGACGGTAGGTTTTAGAACATGCAGAACAAGGCGTATTGGTTTTTCAAGTACATCCTCATCGGCCCG
TGGCTGCGCGTATATAACCG

Product: glucokinase

Products: NA

Alternate protein names: Glucose kinase [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MTGTLTVGVDIGGTNLRAAVVDADGQILDIEKLPTPSNVYALETALVHAINCLQRRNEGIVAVGLAVAGFINSQRDTVRF
APHLPWRNAQVTERLSAKLGLPVELEHDANSAALGEYYLGAAQNAGTWVLFALGTGIGGAVMVNGEIYRGAFGTAPEFGH
LTVMPGGRACPCGKQGCLERYCSGSALALTAQDLIATGRYPDSAITREFAQHPEEISGRTIVRLAREGDPLARAAIEDMG
LWLGRGLSLVQDVFDPELIVIGGGVSSDADLFLDQAESMMNYSIVGAGHRPVAEVKTAQLGGDAGMIGVALLARKHLERG
AE

Sequences:

>Translated_322_residues
MTGTLTVGVDIGGTNLRAAVVDADGQILDIEKLPTPSNVYALETALVHAINCLQRRNEGIVAVGLAVAGFINSQRDTVRF
APHLPWRNAQVTERLSAKLGLPVELEHDANSAALGEYYLGAAQNAGTWVLFALGTGIGGAVMVNGEIYRGAFGTAPEFGH
LTVMPGGRACPCGKQGCLERYCSGSALALTAQDLIATGRYPDSAITREFAQHPEEISGRTIVRLAREGDPLARAAIEDMG
LWLGRGLSLVQDVFDPELIVIGGGVSSDADLFLDQAESMMNYSIVGAGHRPVAEVKTAQLGGDAGMIGVALLARKHLERG
AE
>Mature_321_residues
TGTLTVGVDIGGTNLRAAVVDADGQILDIEKLPTPSNVYALETALVHAINCLQRRNEGIVAVGLAVAGFINSQRDTVRFA
PHLPWRNAQVTERLSAKLGLPVELEHDANSAALGEYYLGAAQNAGTWVLFALGTGIGGAVMVNGEIYRGAFGTAPEFGHL
TVMPGGRACPCGKQGCLERYCSGSALALTAQDLIATGRYPDSAITREFAQHPEEISGRTIVRLAREGDPLARAAIEDMGL
WLGRGLSLVQDVFDPELIVIGGGVSSDADLFLDQAESMMNYSIVGAGHRPVAEVKTAQLGGDAGMIGVALLARKHLERGA
E

Specific function: Required for glucose repression of many different genes [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Homo sapiens, GI298566315, Length=271, Percent_Identity=29.8892988929889, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI298566325, Length=271, Percent_Identity=29.8892988929889, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI4885285, Length=271, Percent_Identity=29.8892988929889, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI190014632, Length=271, Percent_Identity=29.8892988929889, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI1786891, Length=316, Percent_Identity=26.8987341772152, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI87081733, Length=319, Percent_Identity=30.7210031347962, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1787363, Length=323, Percent_Identity=29.7213622291022, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787878, Length=260, Percent_Identity=24.2307692307692, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790522, Length=273, Percent_Identity=27.8388278388278, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI87082127, Length=316, Percent_Identity=23.4177215189873, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87082230, Length=300, Percent_Identity=29, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600
- InterPro:   IPR004654 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.2 [H]

Molecular weight: Translated: 33830; Mature: 33699

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGTLTVGVDIGGTNLRAAVVDADGQILDIEKLPTPSNVYALETALVHAINCLQRRNEGI
CCCEEEEEEEECCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCE
VAVGLAVAGFINSQRDTVRFAPHLPWRNAQVTERLSAKLGLPVELEHDANSAALGEYYLG
EEEHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCHHHHHHC
AAQNAGTWVLFALGTGIGGAVMVNGEIYRGAFGTAPEFGHLTVMPGGRACPCGKQGCLER
CCCCCCCEEEEEECCCCCCEEEECCEEEECCCCCCCCCCEEEECCCCCCCCCCHHHHHHH
YCSGSALALTAQDLIATGRYPDSAITREFAQHPEEISGRTIVRLAREGDPLARAAIEDMG
HCCCCEEEEEHHHHHHCCCCCCHHHHHHHHCCHHHCCCCEEEEEECCCCHHHHHHHHHHH
LWLGRGLSLVQDVFDPELIVIGGGVSSDADLFLDQAESMMNYSIVGAGHRPVAEVKTAQL
HHHHCCHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHC
GGDAGMIGVALLARKHLERGAE
CCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TGTLTVGVDIGGTNLRAAVVDADGQILDIEKLPTPSNVYALETALVHAINCLQRRNEGI
CCEEEEEEEECCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCE
VAVGLAVAGFINSQRDTVRFAPHLPWRNAQVTERLSAKLGLPVELEHDANSAALGEYYLG
EEEHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCHHHHHHC
AAQNAGTWVLFALGTGIGGAVMVNGEIYRGAFGTAPEFGHLTVMPGGRACPCGKQGCLER
CCCCCCCEEEEEECCCCCCEEEECCEEEECCCCCCCCCCEEEECCCCCCCCCCHHHHHHH
YCSGSALALTAQDLIATGRYPDSAITREFAQHPEEISGRTIVRLAREGDPLARAAIEDMG
HCCCCEEEEEHHHHHHCCCCCCHHHHHHHHCCHHHCCCCEEEEEECCCCHHHHHHHHHHH
LWLGRGLSLVQDVFDPELIVIGGGVSSDADLFLDQAESMMNYSIVGAGHRPVAEVKTAQL
HHHHCCHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHC
GGDAGMIGVALLARKHLERGAE
CCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1435260; 12000953 [H]