Click here to switch to the map view.

The map label for this gene is pimB [H]

Identifier: 68535802

GI number: 68535802

Start: 867144

End: 868361

Strand: Direct

Name: pimB [H]

Synonym: jk0727

Alternate gene names: 68535802

Gene position: 867144-868361 (Clockwise)

Preceding gene: 68535801

Following gene: 68535803

Centisome position: 35.21

GC content: 62.81

Gene sequence:

>1218_bases
ATGGCCAAGGAAGATCAGCCCAAAGAGGACCAGCCCCAAGGAGGCCGGGGGAGTCACCCGCGCGTCCTCGTCGTCACCAA
CGATTTCCCGCCGACCTTCGGCGGAATTCAGACCTACGTGCGCGACTACCTTTCTACGCTGAATCCGGAAAACGTCATTG
TCTTCGCTTCCACCCAAAATAGAGAGGAAGCGCAAGCATTCGATGCACAACAGCCTTATCGCGTGATCCGGTGGCCACGC
AGTGTGATGCTGCCAACCCCGGCCACGGCACGGAAAATGCAGAGGCTGATCCGCGAGAACAACATTCATACCGTTTGGTT
CGGTGCTGCCGCACCACTGGCGCTGATGGCAAATGCGGCCCGTGCCGCGGGGGCTCGCCGCGTGGTTGCGTCCACACACG
GCCATGAGGTTGGCTGGTCGATGTTCCCCGGTTCCCGCCAGGTACTGCGGCGCATTGGTAACGCCGTGGATTGTCTGACT
TATGTGTCGCACTACGCCCGCAACCGCATGGCTGCTGCGTTCGGACCTGATGTCGCCTGGGAGCCGATGCCCGGTGGGGT
GAATACCGAATTGTTCCGCCCGGATTCCGTTATGCGGGACACCCTGCGTACCCGCTATGGCCTGGAGGATAAGAAGGTCA
TCGTCGCAGTATCCCGACTGGTTCCGCGCAAAGGCCAGGACACTCTGGTTAGAGCTCTGCCCGCCATCCTGCGTGAGCAC
CCGGATGCGCACCTGTTAGTCGTCGGACCCGGCAAGTACCGCGGGAAGTTGGAGAAACTGGCGCGTCGGTTGGGCGTCAT
AAAGAACACAACATTTACCGGCGCCGTCGATGCGGAGGAACTACCGGGGCATTTCGCGGCCGGTGATGTTTTTGCGCTGC
CTGTGCGAACACAGTTGGGCGGACTGTCGGTGGAAGGCCTTGGCATCGTGTTTTTGGAGGCGCAGGCGGCCGGCGCGGCC
ACAGTCGCCGGCAATGGCGGCGGCGCGCCTGAGACGGTGATCGACGGGGAAACCGGATTCGTCGTGGACTCGCGGAACCT
GGACGACGTTGCAGGGCGTATCTCTTCTTTGCTGGGTGACGCCCCCACGCGCCAGCGCATATCGACCGCGGCGAGGGAAC
ACGTAACGGGGAAGTGGACCTGGGAAGTCCTCGGCCACCAGTGCCGCGCAGTGATGGCCGGAACTGGCGGCATCCCCCCG
CGCCACAGCTTCGCCTGA

Upstream 100 bases:

>100_bases
GCCTCCGACTACGGCATTCCGGTCCAGGTGGTCCCGGTGGACTCTATGCCGATCCAGGGTGCGGCACGCTACTAAGGCGC
GTGCGGTAGCATCCTTGCCT

Downstream 100 bases:

>100_bases
TATCTCGCTGGCCGGGGACGGCAGGTGCAGAATTTCTATGTGCTGCCAGGCTGGCTAGAATGGTGGGGTTAATCGGTACA
GACGAGTATCGGTACAGGCA

Product: putative glycosyltransferase

Products: NA

Alternate protein names: Alpha-D-mannose-alpha-(1-6)-phosphatidylmyo-inositol-mannosyltransferase; Alpha-mannosyltransferase; Alpha-manT; Guanosine diphosphomannose-phosphatidyl-inositol alpha-mannosyltransferase; Phosphatidylinositol alpha-mannosyltransferase; PI alpha-mannosyltransferase [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MAKEDQPKEDQPQGGRGSHPRVLVVTNDFPPTFGGIQTYVRDYLSTLNPENVIVFASTQNREEAQAFDAQQPYRVIRWPR
SVMLPTPATARKMQRLIRENNIHTVWFGAAAPLALMANAARAAGARRVVASTHGHEVGWSMFPGSRQVLRRIGNAVDCLT
YVSHYARNRMAAAFGPDVAWEPMPGGVNTELFRPDSVMRDTLRTRYGLEDKKVIVAVSRLVPRKGQDTLVRALPAILREH
PDAHLLVVGPGKYRGKLEKLARRLGVIKNTTFTGAVDAEELPGHFAAGDVFALPVRTQLGGLSVEGLGIVFLEAQAAGAA
TVAGNGGGAPETVIDGETGFVVDSRNLDDVAGRISSLLGDAPTRQRISTAAREHVTGKWTWEVLGHQCRAVMAGTGGIPP
RHSFA

Sequences:

>Translated_405_residues
MAKEDQPKEDQPQGGRGSHPRVLVVTNDFPPTFGGIQTYVRDYLSTLNPENVIVFASTQNREEAQAFDAQQPYRVIRWPR
SVMLPTPATARKMQRLIRENNIHTVWFGAAAPLALMANAARAAGARRVVASTHGHEVGWSMFPGSRQVLRRIGNAVDCLT
YVSHYARNRMAAAFGPDVAWEPMPGGVNTELFRPDSVMRDTLRTRYGLEDKKVIVAVSRLVPRKGQDTLVRALPAILREH
PDAHLLVVGPGKYRGKLEKLARRLGVIKNTTFTGAVDAEELPGHFAAGDVFALPVRTQLGGLSVEGLGIVFLEAQAAGAA
TVAGNGGGAPETVIDGETGFVVDSRNLDDVAGRISSLLGDAPTRQRISTAAREHVTGKWTWEVLGHQCRAVMAGTGGIPP
RHSFA
>Mature_404_residues
AKEDQPKEDQPQGGRGSHPRVLVVTNDFPPTFGGIQTYVRDYLSTLNPENVIVFASTQNREEAQAFDAQQPYRVIRWPRS
VMLPTPATARKMQRLIRENNIHTVWFGAAAPLALMANAARAAGARRVVASTHGHEVGWSMFPGSRQVLRRIGNAVDCLTY
VSHYARNRMAAAFGPDVAWEPMPGGVNTELFRPDSVMRDTLRTRYGLEDKKVIVAVSRLVPRKGQDTLVRALPAILREHP
DAHLLVVGPGKYRGKLEKLARRLGVIKNTTFTGAVDAEELPGHFAAGDVFALPVRTQLGGLSVEGLGIVFLEAQAAGAAT
VAGNGGGAPETVIDGETGFVVDSRNLDDVAGRISSLLGDAPTRQRISTAAREHVTGKWTWEVLGHQCRAVMAGTGGIPPR
HSFA

Specific function: Catalyzes the addition of a mannose residue from GDP-D- mannose to the position 6 of a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1) to generate phosphatidyl-myo-inositol bearing alpha-1,2- and alpha-1,6-linked mannose resid

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI9755344, Length=228, Percent_Identity=23.2456140350877, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: =2.4.1.57 [H]

Molecular weight: Translated: 43854; Mature: 43722

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEDQPKEDQPQGGRGSHPRVLVVTNDFPPTFGGIQTYVRDYLSTLNPENVIVFASTQN
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCC
REEAQAFDAQQPYRVIRWPRSVMLPTPATARKMQRLIRENNIHTVWFGAAAPLALMANAA
CHHHHHHCCCCCHHHHHCCCCEECCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHH
RAAGARRVVASTHGHEVGWSMFPGSRQVLRRIGNAVDCLTYVSHYARNRMAAAFGPDVAW
HHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCHHHCCCCCCC
EPMPGGVNTELFRPDSVMRDTLRTRYGLEDKKVIVAVSRLVPRKGQDTLVRALPAILREH
CCCCCCCCCCEECCHHHHHHHHHHHCCCCCCHHHEEHHHHCCCCCHHHHHHHHHHHHHCC
PDAHLLVVGPGKYRGKLEKLARRLGVIKNTTFTGAVDAEELPGHFAAGDVFALPVRTQLG
CCCEEEEECCCHHHHHHHHHHHHHCHHHCCCCCCCCCHHHCCCCCCCCCEEEEEEHHHCC
GLSVEGLGIVFLEAQAAGAATVAGNGGGAPETVIDGETGFVVDSRNLDDVAGRISSLLGD
CEEECCCEEEEEEECCCCCEEEECCCCCCCCEEECCCCCEEEECCCHHHHHHHHHHHHCC
APTRQRISTAAREHVTGKWTWEVLGHQCRAVMAGTGGIPPRHSFA
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEECCCCCCCCCCCC
>Mature Secondary Structure 
AKEDQPKEDQPQGGRGSHPRVLVVTNDFPPTFGGIQTYVRDYLSTLNPENVIVFASTQN
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCC
REEAQAFDAQQPYRVIRWPRSVMLPTPATARKMQRLIRENNIHTVWFGAAAPLALMANAA
CHHHHHHCCCCCHHHHHCCCCEECCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHH
RAAGARRVVASTHGHEVGWSMFPGSRQVLRRIGNAVDCLTYVSHYARNRMAAAFGPDVAW
HHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCHHHCCCCCCC
EPMPGGVNTELFRPDSVMRDTLRTRYGLEDKKVIVAVSRLVPRKGQDTLVRALPAILREH
CCCCCCCCCCEECCHHHHHHHHHHHCCCCCCHHHEEHHHHCCCCCHHHHHHHHHHHHHCC
PDAHLLVVGPGKYRGKLEKLARRLGVIKNTTFTGAVDAEELPGHFAAGDVFALPVRTQLG
CCCEEEEECCCHHHHHHHHHHHHHCHHHCCCCCCCCCHHHCCCCCCCCCEEEEEEHHHCC
GLSVEGLGIVFLEAQAAGAATVAGNGGGAPETVIDGETGFVVDSRNLDDVAGRISSLLGD
CEEECCCEEEEEEECCCCCEEEECCCCCCCCEEECCCCCEEEECCCHHHHHHHHHHHHCC
APTRQRISTAAREHVTGKWTWEVLGHQCRAVMAGTGGIPPRHSFA
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]