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The map label for this gene is purR [H]

Identifier: 66767141

GI number: 66767141

Start: 966724

End: 967755

Strand: Reverse

Name: purR [H]

Synonym: XC_0808

Alternate gene names: 66767141

Gene position: 967755-966724 (Counterclockwise)

Preceding gene: 66767142

Following gene: 66767139

Centisome position: 18.8

GC content: 68.51

Gene sequence:

>1032_bases
ATGGCCAAACATCCCGAACGCGCCACTCCTGCTGCACGCCCGCAGATGGCCGACATCGCGCGCATGGCGGGCGTGTCCGA
GTCCACCGTGTCGCGTGCGCTGGCCGGCAGCCCGGTGGTAGCCGAGCGCACCCGTGCCTACATCAAGCAGATCGCCGCCG
ATGCGGGGTATCAGGTCGATCCGGTGGCACGCAGCCTGCGCGCCAAGCGCTCCAACACGGTGAGCGTGGCGGTGCCGATG
ATGCATGCGCTGGACCAGCCGCTGTCGGACCCGTTCCTGATGACCATGCTCGCGCTGCTGGCCGAAGAACTCACCGGCCG
TGGTTACAGCATGCTGTTGTCCAAGCTCAATCGGCATCAGGACGGCTGGGTGGAACAACTCGCACGCGGTAGCCGCTCCG
ATGGCGTGATCGTGCTCGGGCAAAGCTCCGAACATGCCGCGCTGGATGCGGCCGCACGCGATGGCCTGCCGATGGCGGTG
TGGGGCAGCCGGATCGACGGGCAGTCCTACATCAGCGTGGGCAGCGACAATTTCCAGGGCGGCGCACTGGCCACCGAGCA
CCTGATCGCCAGCGGCCGCCGGCGCATCGCCTTCCTGGGCGATGACCAGCTTCCCGAAGTGGCGCCGCGCTTTGCCGGCT
ACCGGCAGGCGCTGGAGCAGCATGGCCTGGAGTTCGACACGCGCCTGCACGCGCGCAGCCACTTCCTCAGCGAGGACGCC
TACCGGCTGACCCGCGCCATGCTCAAGAAGACCGACGCACCCGATGGCCTGTTTGCCGCTTCCGACGTGATTGCGGTCGG
CGCGATCCGCGCCCTGGTCGAAGCCGGCCACCGCGTGCCGCAGGACATTTCGCTGGTGGGCTTCGACGATATTCCGCTGG
CCGCCTACAGCCAGCCGCCGCTGACCACCGTGCAGCAGGACCTGGGCCTGGCCGCGCGCCTGCTGGTGGACCGGCTGCTG
GCATTGATCGCCGGCGAAGCAGTGGACTCGGTGGAGATGCCGGTGAAGCTGGTGGTGCGCGAGTCGGCTTGA

Upstream 100 bases:

>100_bases
GCACCGGCAAATGCCCTGCAAGCGACCGCCACCGCAAGGCCTGCCCGACACGTGGCGTGCACGAACTGATTACACTCTGG
CTTTTCCGCAGTGCGGTGCC

Downstream 100 bases:

>100_bases
TTCGGGATTCGGGATTCGGGATTCGGGATTCGGGATCGGGAATCGGGAATCGGGAATCGGGAATCGGGAATCGGGAATCG
GGAATCGGGAATCGGGAATC

Product: transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MAKHPERATPAARPQMADIARMAGVSESTVSRALAGSPVVAERTRAYIKQIAADAGYQVDPVARSLRAKRSNTVSVAVPM
MHALDQPLSDPFLMTMLALLAEELTGRGYSMLLSKLNRHQDGWVEQLARGSRSDGVIVLGQSSEHAALDAAARDGLPMAV
WGSRIDGQSYISVGSDNFQGGALATEHLIASGRRRIAFLGDDQLPEVAPRFAGYRQALEQHGLEFDTRLHARSHFLSEDA
YRLTRAMLKKTDAPDGLFAASDVIAVGAIRALVEAGHRVPQDISLVGFDDIPLAAYSQPPLTTVQQDLGLAARLLVDRLL
ALIAGEAVDSVEMPVKLVVRESA

Sequences:

>Translated_343_residues
MAKHPERATPAARPQMADIARMAGVSESTVSRALAGSPVVAERTRAYIKQIAADAGYQVDPVARSLRAKRSNTVSVAVPM
MHALDQPLSDPFLMTMLALLAEELTGRGYSMLLSKLNRHQDGWVEQLARGSRSDGVIVLGQSSEHAALDAAARDGLPMAV
WGSRIDGQSYISVGSDNFQGGALATEHLIASGRRRIAFLGDDQLPEVAPRFAGYRQALEQHGLEFDTRLHARSHFLSEDA
YRLTRAMLKKTDAPDGLFAASDVIAVGAIRALVEAGHRVPQDISLVGFDDIPLAAYSQPPLTTVQQDLGLAARLLVDRLL
ALIAGEAVDSVEMPVKLVVRESA
>Mature_342_residues
AKHPERATPAARPQMADIARMAGVSESTVSRALAGSPVVAERTRAYIKQIAADAGYQVDPVARSLRAKRSNTVSVAVPMM
HALDQPLSDPFLMTMLALLAEELTGRGYSMLLSKLNRHQDGWVEQLARGSRSDGVIVLGQSSEHAALDAAARDGLPMAVW
GSRIDGQSYISVGSDNFQGGALATEHLIASGRRRIAFLGDDQLPEVAPRFAGYRQALEQHGLEFDTRLHARSHFLSEDAY
RLTRAMLKKTDAPDGLFAASDVIAVGAIRALVEAGHRVPQDISLVGFDDIPLAAYSQPPLTTVQQDLGLAARLLVDRLLA
LIAGEAVDSVEMPVKLVVRESA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=337, Percent_Identity=29.9703264094956, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1790194, Length=320, Percent_Identity=30, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789068, Length=317, Percent_Identity=30.2839116719243, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1787948, Length=343, Percent_Identity=28.5714285714286, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1786540, Length=337, Percent_Identity=30.5637982195846, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1789456, Length=335, Percent_Identity=29.8507462686567, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788474, Length=320, Percent_Identity=30.3125, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789202, Length=295, Percent_Identity=28.8135593220339, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787580, Length=316, Percent_Identity=25.6329113924051, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787906, Length=362, Percent_Identity=29.0055248618785, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI48994940, Length=319, Percent_Identity=26.0188087774295, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1790715, Length=318, Percent_Identity=26.1006289308176, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1786268, Length=308, Percent_Identity=23.7012987012987, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790689, Length=324, Percent_Identity=23.7654320987654, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36847; Mature: 36715

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKHPERATPAARPQMADIARMAGVSESTVSRALAGSPVVAERTRAYIKQIAADAGYQVD
CCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCC
PVARSLRAKRSNTVSVAVPMMHALDQPLSDPFLMTMLALLAEELTGRGYSMLLSKLNRHQ
HHHHHHHHHCCCCEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
DGWVEQLARGSRSDGVIVLGQSSEHAALDAAARDGLPMAVWGSRIDGQSYISVGSDNFQG
CHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCCCCEEECCCCCCCCCEEEECCCCCCC
GALATEHLIASGRRRIAFLGDDQLPEVAPRFAGYRQALEQHGLEFDTRLHARSHFLSEDA
CHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
YRLTRAMLKKTDAPDGLFAASDVIAVGAIRALVEAGHRVPQDISLVGFDDIPLAAYSQPP
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCC
LTTVQQDLGLAARLLVDRLLALIAGEAVDSVEMPVKLVVRESA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
>Mature Secondary Structure 
AKHPERATPAARPQMADIARMAGVSESTVSRALAGSPVVAERTRAYIKQIAADAGYQVD
CCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCC
PVARSLRAKRSNTVSVAVPMMHALDQPLSDPFLMTMLALLAEELTGRGYSMLLSKLNRHQ
HHHHHHHHHCCCCEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
DGWVEQLARGSRSDGVIVLGQSSEHAALDAAARDGLPMAVWGSRIDGQSYISVGSDNFQG
CHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCCCCEEECCCCCCCCCEEEECCCCCCC
GALATEHLIASGRRRIAFLGDDQLPEVAPRFAGYRQALEQHGLEFDTRLHARSHFLSEDA
CHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
YRLTRAMLKKTDAPDGLFAASDVIAVGAIRALVEAGHRVPQDISLVGFDDIPLAAYSQPP
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCC
LTTVQQDLGLAARLLVDRLLALIAGEAVDSVEMPVKLVVRESA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA