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The map label for this gene is dsbA [H]

Identifier: 66767100

GI number: 66767100

Start: 920189

End: 921010

Strand: Reverse

Name: dsbA [H]

Synonym: XC_0764

Alternate gene names: 66767100

Gene position: 921010-920189 (Counterclockwise)

Preceding gene: 66767101

Following gene: 66767099

Centisome position: 17.89

GC content: 68.0

Gene sequence:

>822_bases
ATGAAGACCCGCTTCGCCCTGACGCTGATGGCGCTGCTGCCGATCCTGGTCGCCTGCAAGGCCCAGGACGGTTCGTCCGA
CACCGCCCCGGCGCCTGCCCCGGCTGCCGAGACCGCCACCGCGCCTGCGGCCACCCCGGCCACTGATGCCGCTGCCCCGC
CTGCCGTGGGCGAGAGCGCCAACGCGCCGGCCACCGACACCGCTGCTGCGCCGCCAGCGCCGGCACCGCGTGCGCCGAAC
GGCCCCGAGCCGGTCGCCGGCACCGATTACCTGGATATCGAAGGTGGCCAGCCGTACCAGCAGGCCGCCGGCAAGATCGA
AGTGGCGGAAGTGTTCGGCTACGTCTGCCCGGCCTGCAACGCGTTCCAGCCGCTGATCGGGCCATGGAAAGCCGGCCTGC
CGTCGGATGTGCATTTCGTCTACGTGCCGGCAATGTTCGGCGGGCCGTGGGATGACTACGCGCGCGCCTTCTACGCCGCA
GAAACGCTGGGCGTGCAGGAAAAGACCCACGACGCGCTGTACAAGGCGATCCACGTCGACCAGAGCCTGAAGGGCGAGCG
CGGCAAGGATTCGGTGCAGGACATCGCCGGTTTCTATGCCAAGTACGGTGTGGATTCGAAGACCTTCATCGACACCATGA
GCAGCTTCGGCGTGTCGGCCAAGACCAACCGCGCCAAGCAGTTCGCCACCCGCAGCAAGATCACCGGCACCCCGTCGCTG
ATCGTCAACGGCAAGTACCTGGTCAAGGGCCAGAGCTTCCCGGACATGCTGCGCATCGCCGATCACCTGATCGCGCGCGA
ACGCGCCACGCTGGCCAAGTGA

Upstream 100 bases:

>100_bases
GCGCTGAGCCGCATAACCGCCATCACGCACGGCCCGTGGCATCATGCGCCCCGCGGCCGCTGCGGCCTCCCCTTCCGCCC
CACGCTGGAGATCCAATCCG

Downstream 100 bases:

>100_bases
CCCGAGCGATCGCTACGTCGTGAACGCTTCGGACTCGCGCACCCTGCGGGTGCTGACCGCCAATATCCAGGCCGGCTCCA
GCACCCGCCGCTACAGTGAC

Product: thiol:disulfide interchange protein

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MKTRFALTLMALLPILVACKAQDGSSDTAPAPAPAAETATAPAATPATDAAAPPAVGESANAPATDTAAAPPAPAPRAPN
GPEPVAGTDYLDIEGGQPYQQAAGKIEVAEVFGYVCPACNAFQPLIGPWKAGLPSDVHFVYVPAMFGGPWDDYARAFYAA
ETLGVQEKTHDALYKAIHVDQSLKGERGKDSVQDIAGFYAKYGVDSKTFIDTMSSFGVSAKTNRAKQFATRSKITGTPSL
IVNGKYLVKGQSFPDMLRIADHLIARERATLAK

Sequences:

>Translated_273_residues
MKTRFALTLMALLPILVACKAQDGSSDTAPAPAPAAETATAPAATPATDAAAPPAVGESANAPATDTAAAPPAPAPRAPN
GPEPVAGTDYLDIEGGQPYQQAAGKIEVAEVFGYVCPACNAFQPLIGPWKAGLPSDVHFVYVPAMFGGPWDDYARAFYAA
ETLGVQEKTHDALYKAIHVDQSLKGERGKDSVQDIAGFYAKYGVDSKTFIDTMSSFGVSAKTNRAKQFATRSKITGTPSL
IVNGKYLVKGQSFPDMLRIADHLIARERATLAK
>Mature_273_residues
MKTRFALTLMALLPILVACKAQDGSSDTAPAPAPAAETATAPAATPATDAAAPPAVGESANAPATDTAAAPPAPAPRAPN
GPEPVAGTDYLDIEGGQPYQQAAGKIEVAEVFGYVCPACNAFQPLIGPWKAGLPSDVHFVYVPAMFGGPWDDYARAFYAA
ETLGVQEKTHDALYKAIHVDQSLKGERGKDSVQDIAGFYAKYGVDSKTFIDTMSSFGVSAKTNRAKQFATRSKITGTPSL
IVNGKYLVKGQSFPDMLRIADHLIARERATLAK

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

None

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 28585; Mature: 28585

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRFALTLMALLPILVACKAQDGSSDTAPAPAPAAETATAPAATPATDAAAPPAVGESA
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
NAPATDTAAAPPAPAPRAPNGPEPVAGTDYLDIEGGQPYQQAAGKIEVAEVFGYVCPACN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHCCCEEHHHHHHHHCCCCC
AFQPLIGPWKAGLPSDVHFVYVPAMFGGPWDDYARAFYAAETLGVQEKTHDALYKAIHVD
CCCHHCCCHHCCCCCCCEEEEEECHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
QSLKGERGKDSVQDIAGFYAKYGVDSKTFIDTMSSFGVSAKTNRAKQFATRSKITGTPSL
HHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEE
IVNGKYLVKGQSFPDMLRIADHLIARERATLAK
EECCEEEEECCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTRFALTLMALLPILVACKAQDGSSDTAPAPAPAAETATAPAATPATDAAAPPAVGESA
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
NAPATDTAAAPPAPAPRAPNGPEPVAGTDYLDIEGGQPYQQAAGKIEVAEVFGYVCPACN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHCCCEEHHHHHHHHCCCCC
AFQPLIGPWKAGLPSDVHFVYVPAMFGGPWDDYARAFYAAETLGVQEKTHDALYKAIHVD
CCCHHCCCHHCCCCCCCEEEEEECHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
QSLKGERGKDSVQDIAGFYAKYGVDSKTFIDTMSSFGVSAKTNRAKQFATRSKITGTPSL
HHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEE
IVNGKYLVKGQSFPDMLRIADHLIARERATLAK
EECCEEEEECCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10051655 [H]