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The map label for this gene is purH [H]

Identifier: 66766849

GI number: 66766849

Start: 601900

End: 603483

Strand: Reverse

Name: purH [H]

Synonym: XC_0510

Alternate gene names: 66766849

Gene position: 603483-601900 (Counterclockwise)

Preceding gene: 66766859

Following gene: 66766848

Centisome position: 11.72

GC content: 67.05

Gene sequence:

>1584_bases
ATGGCCTCTGATTTCCTGCCCGTGCGCCGGGCCCTGCTCTCCGTTTCCGACAAAACCGGCCTGATTGACCTGGCCCGCGC
GCTGGTGGCGCGCAATGTCGAGCTGCTGTCCACCGGCGGCACCGCCAAGGCAATCCGCGATGCGGGCCTGCCGGTGAAGG
ACGTAGCCGAGTTGACCGGTTTCCCGGAAATGATGGACGGCCGCGTCAAGACCCTGCACCCGCTGGTGCACGGCGGCCTG
CTCGGCCGCGCCGGTGTCGACGAAGCAGTAATGGCCGAACACGGCATCGCGCCGATCGACCTGCTGGTGCTCAACCTGTA
TCCGTTCGAGTCGGTCACTGCCAAGGCCGACTGCACGCTGGCCGATGCGGTGGAAAACATCGACATCGGCGGCCCGGCGA
TGCTGCGCAGCGCGGCGAAGAATTTCGCGCGCGTGGCGGTGGCCACCGACCCGGCGCAATACGCCGACCTGCTGGCCGAG
CTGGAGGCCAACAACGGCCAGCTCTCGGCGGCCCAGCGCTTTGCGCTGTCGGTGGCCGCGTTCAACCGCGTGGCGCAGTA
CGACGCGGCGATCAGCAACTATCTCTCGGCCGTGGCCGACAGTGCGGAGAGCGTGCCCACGCGTAGCCCGTTCCCGGCGC
AGATCAATTCCAACTTCATCAAGGTGATGGACCTGCGTTACGGCGAGAACCCGCACCAGTCCGGCGCGTTCTACCGCGAC
CTGTATCCGGTGCCGGGCACGCTGGCGACCTTCCAGCAGCTGCAGGGCAAGGAACTGAGCTACAACAACCTGGCCGATGC
CGATGCGGCGTGGGAATGCGTGCGCCAGTTCGATGCGCCGGCTTGCGTGATCGTCAAGCACGCCAACCCGTGCGGCGTGG
CGGTGGGCGTGGCCTGCGGCGATGCCTATGAGCTGGCCTATGCCACCGACCCCACCAGCGCGTTCGGCGGCATCCTGGCG
TTCAACCGCACGCTGGATGCGGCCACCGCCAAGGCCATCCTGGACCGCCAGTTCGTCGAAGTGCTGATCGCCCCGGACTA
CGAGCCCGGCGCGCTCGACTACGCGACCAAGAAGGCCAACGTGCGCGTGCTCAAGATCCCGCACGGCGCGGGCCTCAACA
ACTACGACACCAAGCGGATCGGCTCTGGCCTGCTGATGCAGTCGGCCGACAACCGCGGCATGTCCCTGGGCGAGCTGAGC
GTGGTGACCAAGCGTGCACCCAGCGATGCCGAACTGGCCGATCTGCTGTTTGCCTGGCGCGTGGCCAAGTACGTCAAATC
CAACGCGATCGTGTATGCCAAGGACAGCCGCACCATTGGTGTGGGCGCCGGGCAGATGAGCCGCGTGGTCAGCGCCAAGA
TCGCCGCGCTCAAGGCCGAAGAAGCCAAGCTTACGGTGGCCGGCTCGGTGATGGCATCGGATGCATTCTTCCCGTTCCGC
GACGGTATCGATGCCGCAGCGGCGGCCGGCATCCAGGCGGTGATCCAGCCGGGCGGCTCGATGCGCGATGGCGAAGTGAT
CGCCGCCGCCGACGAACACGGTCTGGCGATGGTGTTCACCGGCGTGCGCCACTTCCGGCATTGA

Upstream 100 bases:

>100_bases
CTTTCAGAGCATAGATCGAGGCCATACCGGTGGTTGAGTCCATTCGAGCGCTGGCGCAGCCTACAATATCGGCTGCCCCG
CAACGACTGCCCTTTCACCC

Downstream 100 bases:

>100_bases
GATTTTTGATGAATAACTCTGCTACGAATCACTTATTAAAGATTGTCATCGCCGCGAGAGACTTGCGATTACATGCAAGC
TTCATCGATGGATGGAAAAG

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MASDFLPVRRALLSVSDKTGLIDLARALVARNVELLSTGGTAKAIRDAGLPVKDVAELTGFPEMMDGRVKTLHPLVHGGL
LGRAGVDEAVMAEHGIAPIDLLVLNLYPFESVTAKADCTLADAVENIDIGGPAMLRSAAKNFARVAVATDPAQYADLLAE
LEANNGQLSAAQRFALSVAAFNRVAQYDAAISNYLSAVADSAESVPTRSPFPAQINSNFIKVMDLRYGENPHQSGAFYRD
LYPVPGTLATFQQLQGKELSYNNLADADAAWECVRQFDAPACVIVKHANPCGVAVGVACGDAYELAYATDPTSAFGGILA
FNRTLDAATAKAILDRQFVEVLIAPDYEPGALDYATKKANVRVLKIPHGAGLNNYDTKRIGSGLLMQSADNRGMSLGELS
VVTKRAPSDAELADLLFAWRVAKYVKSNAIVYAKDSRTIGVGAGQMSRVVSAKIAALKAEEAKLTVAGSVMASDAFFPFR
DGIDAAAAAGIQAVIQPGGSMRDGEVIAAADEHGLAMVFTGVRHFRH

Sequences:

>Translated_527_residues
MASDFLPVRRALLSVSDKTGLIDLARALVARNVELLSTGGTAKAIRDAGLPVKDVAELTGFPEMMDGRVKTLHPLVHGGL
LGRAGVDEAVMAEHGIAPIDLLVLNLYPFESVTAKADCTLADAVENIDIGGPAMLRSAAKNFARVAVATDPAQYADLLAE
LEANNGQLSAAQRFALSVAAFNRVAQYDAAISNYLSAVADSAESVPTRSPFPAQINSNFIKVMDLRYGENPHQSGAFYRD
LYPVPGTLATFQQLQGKELSYNNLADADAAWECVRQFDAPACVIVKHANPCGVAVGVACGDAYELAYATDPTSAFGGILA
FNRTLDAATAKAILDRQFVEVLIAPDYEPGALDYATKKANVRVLKIPHGAGLNNYDTKRIGSGLLMQSADNRGMSLGELS
VVTKRAPSDAELADLLFAWRVAKYVKSNAIVYAKDSRTIGVGAGQMSRVVSAKIAALKAEEAKLTVAGSVMASDAFFPFR
DGIDAAAAAGIQAVIQPGGSMRDGEVIAAADEHGLAMVFTGVRHFRH
>Mature_526_residues
ASDFLPVRRALLSVSDKTGLIDLARALVARNVELLSTGGTAKAIRDAGLPVKDVAELTGFPEMMDGRVKTLHPLVHGGLL
GRAGVDEAVMAEHGIAPIDLLVLNLYPFESVTAKADCTLADAVENIDIGGPAMLRSAAKNFARVAVATDPAQYADLLAEL
EANNGQLSAAQRFALSVAAFNRVAQYDAAISNYLSAVADSAESVPTRSPFPAQINSNFIKVMDLRYGENPHQSGAFYRDL
YPVPGTLATFQQLQGKELSYNNLADADAAWECVRQFDAPACVIVKHANPCGVAVGVACGDAYELAYATDPTSAFGGILAF
NRTLDAATAKAILDRQFVEVLIAPDYEPGALDYATKKANVRVLKIPHGAGLNNYDTKRIGSGLLMQSADNRGMSLGELSV
VTKRAPSDAELADLLFAWRVAKYVKSNAIVYAKDSRTIGVGAGQMSRVVSAKIAALKAEEAKLTVAGSVMASDAFFPFRD
GIDAAAAAGIQAVIQPGGSMRDGEVIAAADEHGLAMVFTGVRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=512, Percent_Identity=37.6953125, Blast_Score=270, Evalue=2e-72,
Organism=Escherichia coli, GI1790439, Length=524, Percent_Identity=60.8778625954198, Blast_Score=631, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=510, Percent_Identity=35.6862745098039, Blast_Score=258, Evalue=5e-69,
Organism=Caenorhabditis elegans, GI71985574, Length=355, Percent_Identity=27.6056338028169, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71985556, Length=119, Percent_Identity=35.2941176470588, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323768, Length=480, Percent_Identity=37.7083333333333, Blast_Score=279, Evalue=8e-76,
Organism=Saccharomyces cerevisiae, GI6323056, Length=484, Percent_Identity=36.7768595041322, Blast_Score=268, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24649832, Length=507, Percent_Identity=39.6449704142012, Blast_Score=282, Evalue=4e-76,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55657; Mature: 55526

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASDFLPVRRALLSVSDKTGLIDLARALVARNVELLSTGGTAKAIRDAGLPVKDVAELTG
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHCCCCHHHHHHHCC
FPEMMDGRVKTLHPLVHGGLLGRAGVDEAVMAEHGIAPIDLLVLNLYPFESVTAKADCTL
CHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCHH
ADAVENIDIGGPAMLRSAAKNFARVAVATDPAQYADLLAELEANNGQLSAAQRFALSVAA
HHHHHHCCCCCHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
FNRVAQYDAAISNYLSAVADSAESVPTRSPFPAQINSNFIKVMDLRYGENPHQSGAFYRD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEHC
LYPVPGTLATFQQLQGKELSYNNLADADAAWECVRQFDAPACVIVKHANPCGVAVGVACG
CCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECC
DAYELAYATDPTSAFGGILAFNRTLDAATAKAILDRQFVEVLIAPDYEPGALDYATKKAN
CCEEEEEECCCCHHHCCHHEECCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCEECCCC
VRVLKIPHGAGLNNYDTKRIGSGLLMQSADNRGMSLGELSVVTKRAPSDAELADLLFAWR
EEEEEECCCCCCCCCCHHHHCCCCEEECCCCCCCCCHHHHHEECCCCCCHHHHHHHHHHH
VAKYVKSNAIVYAKDSRTIGVGAGQMSRVVSAKIAALKAEEAKLTVAGSVMASDAFFPFR
HHHHHHCCEEEEEECCCEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCCCC
DGIDAAAAAGIQAVIQPGGSMRDGEVIAAADEHGLAMVFTGVRHFRH
CCCCHHHHHHHHHEECCCCCCCCCCEEEEECCCCEEHHHHHHHHHCC
>Mature Secondary Structure 
ASDFLPVRRALLSVSDKTGLIDLARALVARNVELLSTGGTAKAIRDAGLPVKDVAELTG
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHCCCCHHHHHHHCC
FPEMMDGRVKTLHPLVHGGLLGRAGVDEAVMAEHGIAPIDLLVLNLYPFESVTAKADCTL
CHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCHH
ADAVENIDIGGPAMLRSAAKNFARVAVATDPAQYADLLAELEANNGQLSAAQRFALSVAA
HHHHHHCCCCCHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
FNRVAQYDAAISNYLSAVADSAESVPTRSPFPAQINSNFIKVMDLRYGENPHQSGAFYRD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEHC
LYPVPGTLATFQQLQGKELSYNNLADADAAWECVRQFDAPACVIVKHANPCGVAVGVACG
CCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECC
DAYELAYATDPTSAFGGILAFNRTLDAATAKAILDRQFVEVLIAPDYEPGALDYATKKAN
CCEEEEEECCCCHHHCCHHEECCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCEECCCC
VRVLKIPHGAGLNNYDTKRIGSGLLMQSADNRGMSLGELSVVTKRAPSDAELADLLFAWR
EEEEEECCCCCCCCCCHHHHCCCCEEECCCCCCCCCHHHHHEECCCCCCHHHHHHHHHHH
VAKYVKSNAIVYAKDSRTIGVGAGQMSRVVSAKIAALKAEEAKLTVAGSVMASDAFFPFR
HHHHHHCCEEEEEECCCEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCCCC
DGIDAAAAAGIQAVIQPGGSMRDGEVIAAADEHGLAMVFTGVRHFRH
CCCCHHHHHHHHHEECCCCCCCCCCEEEEECCCCEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]