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The map label for this gene is pdhC [H]

Identifier: 66766781

GI number: 66766781

Start: 523475

End: 524983

Strand: Reverse

Name: pdhC [H]

Synonym: XC_0441

Alternate gene names: 66766781

Gene position: 524983-523475 (Counterclockwise)

Preceding gene: 66766782

Following gene: 66766780

Centisome position: 10.2

GC content: 70.18

Gene sequence:

>1509_bases
ATGAGCGAAGCCAAGAATTTCCACCTGCCCGACCTGGGCGAAGGCCTGCCCGACGCCACCATCGTCGAATGGTTCGTCAA
GGAGGGCGACCCCGTGCGCCTGGACGACCCGCTAGTATCGATGGAGACCGCCAAGGCGGTGGTCGAAGTGCCCTCGCCGT
TCTCCGGCACCGTGGTCAAGCTGGCCGGCGCGGCCGGCGATGTGATCACCACCGGTGCGGTGCTGGCGCAGTTCGCGCTG
GATGCCTCGCAACCGCAGCGTGCCGACGGCCAGGACACCGGCCATTCGCATGGACCGGCGCCGACGCACGCACCCACGCC
CAGCACCGGCGACAGCGCGGCTGGGCACACCACACGCGTGGTGGCCTCCGATAACGGCGGCGAAATTGCCGATGCCGATG
CTGGCGATGGCAGCAGCGATCGCGACGATGCCGGCACCGTGGTGGGCGCGATGCAGAGCTCCAACGCCGTGCAGAGCGAG
CAGGCCATTACCGTCGGCGGCGTGCGCGCCATGCCGGTGGTGCGCGCGCTGGCGCGCAAGCTGCGTGTGGACCTGGCACA
GGTGCGCGCTACCGGCCCGGACGGCACGGTCACGCTGGCAGACGTAAAGCAGGCAGCAGCCGCCGGTACCGCGCAGCCCT
CACCCGGCGCGCAGGGCGCGCCGACCTCTCCTGCCCGCGGGAGAGGTGGTCCGATCGATGACGGTGGGAGAGCTGCTGGG
AGCGCTGCCGACGGCAGGGATCGACCCGCAACAGCACCCCTTCTTCCGCCTGCGGGAGAAGGTGCCCGCAGGGCGGACGA
AGGCAACGCCCGCTCCACGCTGTCGGCCAGCGGCAAGCCGATGCGCACCCAGTCGCCCGGCATCAGCGCCAAGGGCCAAC
CCGAGCAACTCAAGGGCGTGCGCCGCAACATGGCACGCGTGATGGCCGATGCGCACAGCAAGGTGGTGCCCACCACGCTC
AACGACGACGCCGACCTGCACGCCTGGCAACCCGGCAACGACGTCACAGTGCGCCTGGTGCGCGGCATCGTGCGCGCCTG
CGAGGCCGTGCCCGCGCTCAACGCCTGGTTCGACGGCGAGGCGCTGAGCCGCACGCTGCACCCGCAGGTGGACATCGGCA
TTGCGGTAGACACCGAAGAAGGCCTGTTAGTGCCGGCACTGCGCAACGCCGACATGCTGGATGCGCATGGCATTCGCGAA
GGCGTCAACCGGCTGCGCCAGCAGGTGGAAAGCCGCAGCATCGCCGCGTCCGAACTGAGCGGCTACACCATCTCGCTGTC
CAACTTCGGCATGTTTGCCGGCCGCTACGCCACGCCGGTGGTGGTGCCGCCCTGCGTGGCGATCGTGGCCGCCGGCCGCG
CGCGCTACCAGCTCACCCCGGTGATGGGCGGCGTGGAAACCCACAAGGTGATGCCGCTGTCGCTGACCTTCGATCACCGC
GCCGCCACCGGCGGTGAAGCGGCGCGCTTCCTGCGCGCCTTGCTGGACGATCTGGCGTTGGCCAACTGA

Upstream 100 bases:

>100_bases
CCGAGCGTGCCGATGGCACGCAGGGCTGGCTGCCGGCCGCCGACCTCGAACTCCTCGACGACACCACGCCGCAGCTGCAG
ACGGCGCAAGAGAACCTGCC

Downstream 100 bases:

>100_bases
GCACTGTCTTTTTTGCCATGTAGCACGATGTGCGTCACCGTGACAGCGGCAACATTGGCCGCTGTCCGATGCACATGCTG
CACCGGCCCGGAACACTCAT

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MSEAKNFHLPDLGEGLPDATIVEWFVKEGDPVRLDDPLVSMETAKAVVEVPSPFSGTVVKLAGAAGDVITTGAVLAQFAL
DASQPQRADGQDTGHSHGPAPTHAPTPSTGDSAAGHTTRVVASDNGGEIADADAGDGSSDRDDAGTVVGAMQSSNAVQSE
QAITVGGVRAMPVVRALARKLRVDLAQVRATGPDGTVTLADVKQAAAAGTAQPSPGAQGAPTSPARGRGGPIDDGGRAAG
SAADGRDRPATAPLLPPAGEGARRADEGNARSTLSASGKPMRTQSPGISAKGQPEQLKGVRRNMARVMADAHSKVVPTTL
NDDADLHAWQPGNDVTVRLVRGIVRACEAVPALNAWFDGEALSRTLHPQVDIGIAVDTEEGLLVPALRNADMLDAHGIRE
GVNRLRQQVESRSIAASELSGYTISLSNFGMFAGRYATPVVVPPCVAIVAAGRARYQLTPVMGGVETHKVMPLSLTFDHR
AATGGEAARFLRALLDDLALAN

Sequences:

>Translated_502_residues
MSEAKNFHLPDLGEGLPDATIVEWFVKEGDPVRLDDPLVSMETAKAVVEVPSPFSGTVVKLAGAAGDVITTGAVLAQFAL
DASQPQRADGQDTGHSHGPAPTHAPTPSTGDSAAGHTTRVVASDNGGEIADADAGDGSSDRDDAGTVVGAMQSSNAVQSE
QAITVGGVRAMPVVRALARKLRVDLAQVRATGPDGTVTLADVKQAAAAGTAQPSPGAQGAPTSPARGRGGPIDDGGRAAG
SAADGRDRPATAPLLPPAGEGARRADEGNARSTLSASGKPMRTQSPGISAKGQPEQLKGVRRNMARVMADAHSKVVPTTL
NDDADLHAWQPGNDVTVRLVRGIVRACEAVPALNAWFDGEALSRTLHPQVDIGIAVDTEEGLLVPALRNADMLDAHGIRE
GVNRLRQQVESRSIAASELSGYTISLSNFGMFAGRYATPVVVPPCVAIVAAGRARYQLTPVMGGVETHKVMPLSLTFDHR
AATGGEAARFLRALLDDLALAN
>Mature_501_residues
SEAKNFHLPDLGEGLPDATIVEWFVKEGDPVRLDDPLVSMETAKAVVEVPSPFSGTVVKLAGAAGDVITTGAVLAQFALD
ASQPQRADGQDTGHSHGPAPTHAPTPSTGDSAAGHTTRVVASDNGGEIADADAGDGSSDRDDAGTVVGAMQSSNAVQSEQ
AITVGGVRAMPVVRALARKLRVDLAQVRATGPDGTVTLADVKQAAAAGTAQPSPGAQGAPTSPARGRGGPIDDGGRAAGS
AADGRDRPATAPLLPPAGEGARRADEGNARSTLSASGKPMRTQSPGISAKGQPEQLKGVRRNMARVMADAHSKVVPTTLN
DDADLHAWQPGNDVTVRLVRGIVRACEAVPALNAWFDGEALSRTLHPQVDIGIAVDTEEGLLVPALRNADMLDAHGIREG
VNRLRQQVESRSIAASELSGYTISLSNFGMFAGRYATPVVVPPCVAIVAAGRARYQLTPVMGGVETHKVMPLSLTFDHRA
ATGGEAARFLRALLDDLALAN

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI203098816, Length=507, Percent_Identity=23.0769230769231, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI203098753, Length=507, Percent_Identity=23.0769230769231, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI19923748, Length=215, Percent_Identity=25.1162790697674, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI110671329, Length=228, Percent_Identity=29.3859649122807, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI31711992, Length=156, Percent_Identity=29.4871794871795, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI260898739, Length=162, Percent_Identity=29.6296296296296, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1786946, Length=161, Percent_Identity=31.055900621118, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1786305, Length=166, Percent_Identity=27.1084337349398, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17537937, Length=494, Percent_Identity=23.4817813765182, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI25146366, Length=157, Percent_Identity=33.1210191082803, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17538894, Length=162, Percent_Identity=30.2469135802469, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17560088, Length=162, Percent_Identity=29.6296296296296, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320352, Length=155, Percent_Identity=30.3225806451613, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324258, Length=211, Percent_Identity=27.9620853080569, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24645909, Length=144, Percent_Identity=32.6388888888889, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24582497, Length=164, Percent_Identity=31.0975609756098, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI20129315, Length=164, Percent_Identity=31.0975609756098, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI18859875, Length=214, Percent_Identity=27.1028037383178, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 51589; Mature: 51458

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAKNFHLPDLGEGLPDATIVEWFVKEGDPVRLDDPLVSMETAKAVVEVPSPFSGTVVK
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEECCCCHHHHHHHHHEECCCCCCCCEEE
LAGAAGDVITTGAVLAQFALDASQPQRADGQDTGHSHGPAPTHAPTPSTGDSAAGHTTRV
EECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
VASDNGGEIADADAGDGSSDRDDAGTVVGAMQSSNAVQSEQAITVGGVRAMPVVRALARK
EEECCCCCEECCCCCCCCCCCCCCCCEEEEHHCCCCCCCCCEEEECCCHHHHHHHHHHHH
LRVDLAQVRATGPDGTVTLADVKQAAAAGTAQPSPGAQGAPTSPARGRGGPIDDGGRAAG
HHHHHHHHEECCCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SAADGRDRPATAPLLPPAGEGARRADEGNARSTLSASGKPMRTQSPGISAKGQPEQLKGV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHH
RRNMARVMADAHSKVVPTTLNDDADLHAWQPGNDVTVRLVRGIVRACEAVPALNAWFDGE
HHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCHHCCCCCH
ALSRTLHPQVDIGIAVDTEEGLLVPALRNADMLDAHGIREGVNRLRQQVESRSIAASELS
HHHHHCCCCEEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GYTISLSNFGMFAGRYATPVVVPPCVAIVAAGRARYQLTPVMGGVETHKVMPLSLTFDHR
CCEEEECCCCCCCCCCCCCEEHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEEECCC
AATGGEAARFLRALLDDLALAN
CCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEAKNFHLPDLGEGLPDATIVEWFVKEGDPVRLDDPLVSMETAKAVVEVPSPFSGTVVK
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEECCCCHHHHHHHHHEECCCCCCCCEEE
LAGAAGDVITTGAVLAQFALDASQPQRADGQDTGHSHGPAPTHAPTPSTGDSAAGHTTRV
EECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
VASDNGGEIADADAGDGSSDRDDAGTVVGAMQSSNAVQSEQAITVGGVRAMPVVRALARK
EEECCCCCEECCCCCCCCCCCCCCCCEEEEHHCCCCCCCCCEEEECCCHHHHHHHHHHHH
LRVDLAQVRATGPDGTVTLADVKQAAAAGTAQPSPGAQGAPTSPARGRGGPIDDGGRAAG
HHHHHHHHEECCCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SAADGRDRPATAPLLPPAGEGARRADEGNARSTLSASGKPMRTQSPGISAKGQPEQLKGV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHH
RRNMARVMADAHSKVVPTTLNDDADLHAWQPGNDVTVRLVRGIVRACEAVPALNAWFDGE
HHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCHHCCCCCH
ALSRTLHPQVDIGIAVDTEEGLLVPALRNADMLDAHGIREGVNRLRQQVESRSIAASELS
HHHHHCCCCEEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GYTISLSNFGMFAGRYATPVVVPPCVAIVAAGRARYQLTPVMGGVETHKVMPLSLTFDHR
CCEEEECCCCCCCCCCCCCEEHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEEECCC
AATGGEAARFLRALLDDLALAN
CCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA