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The map label for this gene is bioB [H]

Identifier: 66766743

GI number: 66766743

Start: 462400

End: 463434

Strand: Reverse

Name: bioB [H]

Synonym: XC_0400

Alternate gene names: 66766743

Gene position: 463434-462400 (Counterclockwise)

Preceding gene: 66766745

Following gene: 66766742

Centisome position: 9.0

GC content: 67.44

Gene sequence:

>1035_bases
ATGTCCGTTGTCGTCCGCCATGACTGGGATCGCAAAGAACTGCATGCGCTGTTCGCGCTGCCGTTTCCCGAGCTGCTGCA
TCGCGCCGCCAGCGTGCACCGTGCCCACTTCGACCCGGCCGAGGTGCAGGTCTCCACGCTGCTGTCGGTCAAGACCGGCG
GCTGCCCGGAAGATTGCGCCTACTGCCCGCAGGCGCAGCGCTACGACACCGGGGTGAGCGCACAGAAACTGATGGACACC
GAAGACGTGGTGGCCAAGGCCCGCCAGGCAAAGGCCGCCGGCGCCTCGCGCTTCTGCATGGGTGCGGCGTGGCGCTCGCC
CAAGGACCGCGACATCCCCAAGGTGGCCGCCATGATCCGCGAGGTGAAGGCGATGGGCCTGGAAACCTGCGCCACCCTGG
GCATGCTCGACGCCGGCCAGGCGCGCGCGCTCAAGGACGCCGGGCTGGACTACTACAACCACAATCTGGACACCGCGCCG
GATTACTACGACTCGATCATCCACACCCGCCAGTACCAAGACCGCCTGAACACCCTGGAGCACGTGCGCGATGTGGGCTT
GAAGACCTGCTGCGGCGGCATCGTCGGCATGGGCGAAACCCGTGAGCACCGCGTCGGCTTGTTGCACGCGCTGGCAACGT
TGCCAGCGCACCCGGACTCGGTGCCGATCAACCAGTTGGTGCAGGTTCCCGGCACTCCATTGCACGGCACCGAGCCCCTG
GATGCATTCGAGTTCGTGCGCATGATCGCGGTGGCGCGCATCGCCATGCCCAAGTCGATGGTGCGCCTGTCCGCCGGCCG
CGAGGCGATGAGCGACGAACTGCAGGCGCTGTGCTTCCTGGCCGGTGCCAACTCGATCTTCTACGGCGAAAAGCTGCTCA
CCACCGGCAACCCTGATACCGAGCGCGACCAGGGGTTGTTCCAGCGGCTGGGCCTGCGCCCGATGCAGATCACCGTCGAC
GCCGCCGAGCACGACCACCCCGGCACCGTGCATGCGGACATCACCTGCGGCGCGGCGTGCGAACACGCGGCCTGA

Upstream 100 bases:

>100_bases
TGGCCACCTGTAAACCGAATGCGGATGCGTAAAGTTGACAGCCTCGTCCATGCTCACCAGACTGCCTGGCTTCCAAGCCG
CTGACTGTCAGGAAACCCCG

Downstream 100 bases:

>100_bases
CCGCATCAGCGCCCAGCTAGCACCGGCCGTCGGTGCCGGCTGGGCGCGCTGGGCCCGGCACGCTACGCTAGCGGCCCTCT
TCCGCTGTCGACGAACGCCA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MSVVVRHDWDRKELHALFALPFPELLHRAASVHRAHFDPAEVQVSTLLSVKTGGCPEDCAYCPQAQRYDTGVSAQKLMDT
EDVVAKARQAKAAGASRFCMGAAWRSPKDRDIPKVAAMIREVKAMGLETCATLGMLDAGQARALKDAGLDYYNHNLDTAP
DYYDSIIHTRQYQDRLNTLEHVRDVGLKTCCGGIVGMGETREHRVGLLHALATLPAHPDSVPINQLVQVPGTPLHGTEPL
DAFEFVRMIAVARIAMPKSMVRLSAGREAMSDELQALCFLAGANSIFYGEKLLTTGNPDTERDQGLFQRLGLRPMQITVD
AAEHDHPGTVHADITCGAACEHAA

Sequences:

>Translated_344_residues
MSVVVRHDWDRKELHALFALPFPELLHRAASVHRAHFDPAEVQVSTLLSVKTGGCPEDCAYCPQAQRYDTGVSAQKLMDT
EDVVAKARQAKAAGASRFCMGAAWRSPKDRDIPKVAAMIREVKAMGLETCATLGMLDAGQARALKDAGLDYYNHNLDTAP
DYYDSIIHTRQYQDRLNTLEHVRDVGLKTCCGGIVGMGETREHRVGLLHALATLPAHPDSVPINQLVQVPGTPLHGTEPL
DAFEFVRMIAVARIAMPKSMVRLSAGREAMSDELQALCFLAGANSIFYGEKLLTTGNPDTERDQGLFQRLGLRPMQITVD
AAEHDHPGTVHADITCGAACEHAA
>Mature_343_residues
SVVVRHDWDRKELHALFALPFPELLHRAASVHRAHFDPAEVQVSTLLSVKTGGCPEDCAYCPQAQRYDTGVSAQKLMDTE
DVVAKARQAKAAGASRFCMGAAWRSPKDRDIPKVAAMIREVKAMGLETCATLGMLDAGQARALKDAGLDYYNHNLDTAPD
YYDSIIHTRQYQDRLNTLEHVRDVGLKTCCGGIVGMGETREHRVGLLHALATLPAHPDSVPINQLVQVPGTPLHGTEPLD
AFEFVRMIAVARIAMPKSMVRLSAGREAMSDELQALCFLAGANSIFYGEKLLTTGNPDTERDQGLFQRLGLRPMQITVDA
AEHDHPGTVHADITCGAACEHAA

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=321, Percent_Identity=59.8130841121495, Blast_Score=415, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6321725, Length=318, Percent_Identity=44.6540880503145, Blast_Score=319, Evalue=5e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 37516; Mature: 37385

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVVRHDWDRKELHALFALPFPELLHRAASVHRAHFDPAEVQVSTLLSVKTGGCPEDCA
CEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHEECCCCCCHHHH
YCPQAQRYDTGVSAQKLMDTEDVVAKARQAKAAGASRFCMGAAWRSPKDRDIPKVAAMIR
CCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHH
EVKAMGLETCATLGMLDAGQARALKDAGLDYYNHNLDTAPDYYDSIIHTRQYQDRLNTLE
HHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
HVRDVGLKTCCGGIVGMGETREHRVGLLHALATLPAHPDSVPINQLVQVPGTPLHGTEPL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCC
DAFEFVRMIAVARIAMPKSMVRLSAGREAMSDELQALCFLAGANSIFYGEKLLTTGNPDT
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEECCEEEECCCCCC
ERDQGLFQRLGLRPMQITVDAAEHDHPGTVHADITCGAACEHAA
HHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure 
SVVVRHDWDRKELHALFALPFPELLHRAASVHRAHFDPAEVQVSTLLSVKTGGCPEDCA
EEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHEECCCCCCHHHH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HHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]