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The map label for this gene is bioH

Identifier: 66766740

GI number: 66766740

Start: 459934

End: 460695

Strand: Reverse

Name: bioH

Synonym: XC_0397

Alternate gene names: 66766740

Gene position: 460695-459934 (Counterclockwise)

Preceding gene: 66766741

Following gene: 66766739

Centisome position: 8.95

GC content: 71.26

Gene sequence:

>762_bases
ATGCATATCGACGTCATCGGCCACGGGCCGGCACTGGTTCTTCTGCACGGCTGGGCACTGCATGGCGGTGTGTTCGCACC
CTTGGTGGAGCGCCTGGCACCGCACTACCAACTGCATCTGGTGGACCTGCCCGGGCATGGCTTCAGCCACGACGACACTA
CGCCGCTGGCCTTGCCGCACGTGGTGGCCGCAATCGCCGCGGCCACGCCGGCGGCGGTCTGGGTGGGCTGGTCGTTGGGC
GGCCTGTTCGCACTGCATGCAGCGGCCACCCAACCGCAGGTCCGCGCGCTGGCGATGATCGCCGCCACGCCGCGCTTCGT
GCGTGGCAGCGACTGGCCCGATGCGGTGGAACGCGAGGTCTTCGTGCAATTCGGCCAGGATCTTGCGCGCGATTACCGCG
GCACGCTCGACCGGTTTCTCGCCCTGGACACGCTGGGCTCGGCGCATGCGCGCAGCGAATTGCGCAGCCTGCGCGAGACG
CTCACCGCGCGCGGCGAACCGGCAGCCAGCGCGCTGCAGGATGGCCTGGGCCTGCTCGAACGCACCGACCTGCGCCGCGC
GCTGGCAACGCTTGCGCGGCCCAGCCTGTGGATCGCCGGGCAACGCGACCGCCTGGTGCCGGCCGCCGGCATGCATGCCG
CGGCTGCGCGCGCGCCACACGCACAGGCACTCACCATCGACGGCGGCGGACATGCACCGTTCCTGGGCCACGCCGACCAG
GTGGCCGAGGCGTTGCACCGCTTTGTTGCAGCGCTGCCCTGA

Upstream 100 bases:

>100_bases
GCACGTGCGCAGTGGCGTTGGCTTGGCCCACGCGTGGCCGCGTTGCGCGCCGAGGCCGCAGCTACCTGACCCAGCACGCG
TGATCGGCGACACTACGCGC

Downstream 100 bases:

>100_bases
GAGATGCACACCGTGTGTGATGCGACGCGGCAGCAAGGTGCATCTGCCCGCGCGCAGGTGCATGGCGAAGGCTTCACGTC
GGCCTGCGGCGTGTAGTGAG

Product: biotin biosynthesis protein

Products: NA

Alternate protein names: Biotin synthesis protein BioH

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MHIDVIGHGPALVLLHGWALHGGVFAPLVERLAPHYQLHLVDLPGHGFSHDDTTPLALPHVVAAIAAATPAAVWVGWSLG
GLFALHAAATQPQVRALAMIAATPRFVRGSDWPDAVEREVFVQFGQDLARDYRGTLDRFLALDTLGSAHARSELRSLRET
LTARGEPAASALQDGLGLLERTDLRRALATLARPSLWIAGQRDRLVPAAGMHAAAARAPHAQALTIDGGGHAPFLGHADQ
VAEALHRFVAALP

Sequences:

>Translated_253_residues
MHIDVIGHGPALVLLHGWALHGGVFAPLVERLAPHYQLHLVDLPGHGFSHDDTTPLALPHVVAAIAAATPAAVWVGWSLG
GLFALHAAATQPQVRALAMIAATPRFVRGSDWPDAVEREVFVQFGQDLARDYRGTLDRFLALDTLGSAHARSELRSLRET
LTARGEPAASALQDGLGLLERTDLRRALATLARPSLWIAGQRDRLVPAAGMHAAAARAPHAQALTIDGGGHAPFLGHADQ
VAEALHRFVAALP
>Mature_253_residues
MHIDVIGHGPALVLLHGWALHGGVFAPLVERLAPHYQLHLVDLPGHGFSHDDTTPLALPHVVAAIAAATPAAVWVGWSLG
GLFALHAAATQPQVRALAMIAATPRFVRGSDWPDAVEREVFVQFGQDLARDYRGTLDRFLALDTLGSAHARSELRSLRET
LTARGEPAASALQDGLGLLERTDLRRALATLARPSLWIAGQRDRLVPAAGMHAAAARAPHAQALTIDGGGHAPFLGHADQ
VAEALHRFVAALP

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family

Homologues:

Organism=Escherichia coli, GI1789817, Length=241, Percent_Identity=39.0041493775934, Blast_Score=153, Evalue=9e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOH_XANC8 (Q4UZP1)

Other databases:

- EMBL:   CP000050
- RefSeq:   YP_241502.1
- HSSP:   P13001
- ProteinModelPortal:   Q4UZP1
- STRING:   Q4UZP1
- GeneID:   3381594
- GenomeReviews:   CP000050_GR
- KEGG:   xcb:XC_0397
- eggNOG:   COG0596
- HOGENOM:   HBG748003
- OMA:   CTIASSP
- PhylomeDB:   Q4UZP1
- ProtClustDB:   CLSK446229
- BioCyc:   XCAM314565:XC_0397-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01260
- InterPro:   IPR000073
- InterPro:   IPR010076
- TIGRFAMs:   TIGR01738

Pfam domain/function: PF00561 Abhydrolase_1

EC number: =3.1.1.1

Molecular weight: Translated: 26850; Mature: 26850

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: ACT_SITE 78-78 ACT_SITE 203-203 ACT_SITE 231-231

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIDVIGHGPALVLLHGWALHGGVFAPLVERLAPHYQLHLVDLPGHGFSHDDTTPLALPH
CEEEEECCCCEEEEEECHHHHCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHH
VVAAIAAATPAAVWVGWSLGGLFALHAAATQPQVRALAMIAATPRFVRGSDWPDAVEREV
HHHHHHHCCCHHHEEHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
FVQFGQDLARDYRGTLDRFLALDTLGSAHARSELRSLRETLTARGEPAASALQDGLGLLE
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RTDLRRALATLARPSLWIAGQRDRLVPAAGMHAAAARAPHAQALTIDGGGHAPFLGHADQ
HHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCHHH
VAEALHRFVAALP
HHHHHHHHHHCCC
>Mature Secondary Structure
MHIDVIGHGPALVLLHGWALHGGVFAPLVERLAPHYQLHLVDLPGHGFSHDDTTPLALPH
CEEEEECCCCEEEEEECHHHHCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHH
VVAAIAAATPAAVWVGWSLGGLFALHAAATQPQVRALAMIAATPRFVRGSDWPDAVEREV
HHHHHHHCCCHHHEEHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
FVQFGQDLARDYRGTLDRFLALDTLGSAHARSELRSLRETLTARGEPAASALQDGLGLLE
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RTDLRRALATLARPSLWIAGQRDRLVPAAGMHAAAARAPHAQALTIDGGGHAPFLGHADQ
HHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCHHH
VAEALHRFVAALP
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA