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The map label for this gene is metR [H]

Identifier: 66766670

GI number: 66766670

Start: 386987

End: 387892

Strand: Reverse

Name: metR [H]

Synonym: XC_0327

Alternate gene names: 66766670

Gene position: 387892-386987 (Counterclockwise)

Preceding gene: 66766675

Following gene: 66766669

Centisome position: 7.53

GC content: 64.68

Gene sequence:

>906_bases
ATGCTTGAGCGCATCCATCTTTCCATCGTCCAGCAGGTCGAGCAGCAGGGCTCGCTGACGGCCGCCGCAGGCGTGTTGAA
CCTGACCCAGTCGGCGCTGAGCCACAGCATGAAAAAGCTGGAGCAGCAGCTGGGCACCGATGTCTGGCTGCGCGAGGGGC
GCAGCTTGCGGCTGACCCAGGCTGGCCAGTATTTGCTGGCGGTGGCCAACCGGGTGCTGCCGCAGCTGGATCTGGCCGAA
GAGCGCCTGGGGCAGTTCGCGCAGGGCGAACGCGGTGCGCTGCGCATCGGCATGGAATGCCATCCCTGCTACCAGTGGCT
GCTCAAACTGGTGGCGCCCTATCTGGCCACGTGGCCGGATGTGGATGTGGACGTCAAACAAAAGTTCCAGTTCGGCGGCA
TTGGTGCCCTGTTCGGCTACGAGATCGATCTGCTGGTCACGCCCGACCCGCTGTACAAGCCCGGGCTGCGCTTCGAGCCG
GTGTTCGACTACGAGCAGGTGCTTGTGGTGGCCAGCGGTCATGCGCTGGCGTCGGCCGCCTACGTCACGCCGCCGCAGCT
GACACGCGAGGTGCTCATCAGCTATCCGGTGGACCTGGAGCGGTTGGATATCTATAGCCAGTTCCTGCTGCCGGCCGGTG
TCACGCCCAGGCGCCACAAAGCTATCGAAACCACCGACATCATGATGCAGATGGTGGCCAGTGGCCGCGGTGTGGCCGCC
ATGCCGCGCTGGCTGGTGGAGGAATATGCCGCCAGGATGGACGTGGTGCCGGTTCGGCTGGGTGCACGTGGCATCGCCAA
GCAAATTTTTCTGGGGGCGCGCGAGGCAGATATCGCCATCGACTACCTGCGCGCCTTTATCGAACTGGCACGCCAGCCTG
GCAATGGCAGCGGCACTGCGCCGTGA

Upstream 100 bases:

>100_bases
CATGCAGATGGCCTTAAAACGACGAACCCCGATGCTAGGGCGCGCATTCGATGAAGTAAAATGGTGTTATTTCAACAATC
CATGAATTCGGATCATCTGA

Downstream 100 bases:

>100_bases
CTCCACCGACGGACACCACCGCACAGGCGCGCGTGTTGCATGGCTGCCCTCCCGCACGATCAACGCTGATGTGCGCGAGC
CAACCACTCATCACCACAGG

Product: MetE/MetH family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MLERIHLSIVQQVEQQGSLTAAAGVLNLTQSALSHSMKKLEQQLGTDVWLREGRSLRLTQAGQYLLAVANRVLPQLDLAE
ERLGQFAQGERGALRIGMECHPCYQWLLKLVAPYLATWPDVDVDVKQKFQFGGIGALFGYEIDLLVTPDPLYKPGLRFEP
VFDYEQVLVVASGHALASAAYVTPPQLTREVLISYPVDLERLDIYSQFLLPAGVTPRRHKAIETTDIMMQMVASGRGVAA
MPRWLVEEYAARMDVVPVRLGARGIAKQIFLGAREADIAIDYLRAFIELARQPGNGSGTAP

Sequences:

>Translated_301_residues
MLERIHLSIVQQVEQQGSLTAAAGVLNLTQSALSHSMKKLEQQLGTDVWLREGRSLRLTQAGQYLLAVANRVLPQLDLAE
ERLGQFAQGERGALRIGMECHPCYQWLLKLVAPYLATWPDVDVDVKQKFQFGGIGALFGYEIDLLVTPDPLYKPGLRFEP
VFDYEQVLVVASGHALASAAYVTPPQLTREVLISYPVDLERLDIYSQFLLPAGVTPRRHKAIETTDIMMQMVASGRGVAA
MPRWLVEEYAARMDVVPVRLGARGIAKQIFLGAREADIAIDYLRAFIELARQPGNGSGTAP
>Mature_301_residues
MLERIHLSIVQQVEQQGSLTAAAGVLNLTQSALSHSMKKLEQQLGTDVWLREGRSLRLTQAGQYLLAVANRVLPQLDLAE
ERLGQFAQGERGALRIGMECHPCYQWLLKLVAPYLATWPDVDVDVKQKFQFGGIGALFGYEIDLLVTPDPLYKPGLRFEP
VFDYEQVLVVASGHALASAAYVTPPQLTREVLISYPVDLERLDIYSQFLLPAGVTPRRHKAIETTDIMMQMVASGRGVAA
MPRWLVEEYAARMDVVPVRLGARGIAKQIFLGAREADIAIDYLRAFIELARQPGNGSGTAP

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes. It is also a negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=292, Percent_Identity=40.4109589041096, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI157672245, Length=217, Percent_Identity=29.0322580645161, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI2367136, Length=224, Percent_Identity=26.7857142857143, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787879, Length=260, Percent_Identity=24.6153846153846, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786401, Length=292, Percent_Identity=22.6027397260274, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787530, Length=284, Percent_Identity=23.2394366197183, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789204, Length=261, Percent_Identity=29.1187739463602, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787589, Length=254, Percent_Identity=24.0157480314961, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789639, Length=255, Percent_Identity=26.2745098039216, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789173, Length=229, Percent_Identity=27.0742358078603, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33311; Mature: 33311

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLERIHLSIVQQVEQQGSLTAAAGVLNLTQSALSHSMKKLEQQLGTDVWLREGRSLRLTQ
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEHH
AGQYLLAVANRVLPQLDLAEERLGQFAQGERGALRIGMECHPCYQWLLKLVAPYLATWPD
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCC
VDVDVKQKFQFGGIGALFGYEIDLLVTPDPLYKPGLRFEPVFDYEQVLVVASGHALASAA
CCCCHHHHHCCCCEEEEECEEEEEEECCCCCCCCCCCCCCCCCHHEEEEEECCCHHHHEE
YVTPPQLTREVLISYPVDLERLDIYSQFLLPAGVTPRRHKAIETTDIMMQMVASGRGVAA
ECCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHH
MPRWLVEEYAARMDVVPVRLGARGIAKQIFLGAREADIAIDYLRAFIELARQPGNGSGTA
HHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
P
C
>Mature Secondary Structure
MLERIHLSIVQQVEQQGSLTAAAGVLNLTQSALSHSMKKLEQQLGTDVWLREGRSLRLTQ
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEHH
AGQYLLAVANRVLPQLDLAEERLGQFAQGERGALRIGMECHPCYQWLLKLVAPYLATWPD
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCC
VDVDVKQKFQFGGIGALFGYEIDLLVTPDPLYKPGLRFEPVFDYEQVLVVASGHALASAA
CCCCHHHHHCCCCEEEEECEEEEEEECCCCCCCCCCCCCCCCCHHEEEEEECCCHHHHEE
YVTPPQLTREVLISYPVDLERLDIYSQFLLPAGVTPRRHKAIETTDIMMQMVASGRGVAA
ECCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHH
MPRWLVEEYAARMDVVPVRLGARGIAKQIFLGAREADIAIDYLRAFIELARQPGNGSGTA
HHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]