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The map label for this gene is mcbR [H]

Identifier: 66045423

GI number: 66045423

Start: 2537453

End: 2538175

Strand: Direct

Name: mcbR [H]

Synonym: Psyr_2183

Alternate gene names: 66045423

Gene position: 2537453-2538175 (Clockwise)

Preceding gene: 66045422

Following gene: 66045424

Centisome position: 41.64

GC content: 62.38

Gene sequence:

>723_bases
ATGACAGACCGTCCCATCCTCCTGCCGCCCATGCGCCAGGTCTCTCGCGATACCCTCCAGGACCAGGTCTACCGACAGAT
TCGTGAAGCACTGATGAGCGGGCGCTTTCAGCCGGGTCAGAAGCTGACGATCCGTGGTCTGGCCGAGGCGCTGGGTTCCA
GTCCGATGCCGGTGCGCGAGGCGTTGAATCGTCTTAGCGCCGAGAACGCCTTCGAAGTCACCGAAACCGCCCGGCTGCGT
GTGCCCATGATAACCCCGGAGCGGTTGCGCGAGATTCGCGACGCGCGGGTGGCGCTGGAAGGTCTGTTGGCCGAGAAAGC
CGTGGTTCTGATCAACGACGCCGATCTTGAAGACATCAGCCAGCTCTGCGGGCAGATGCAGAAGGCTGCCGACAGCGTTG
ATGTCGCGCTCTATCTGTGGACCAACTTTGCCTTTCACCGGCGCATTTACGCGGTGGCCAAGGCTGAGCTGATCGTTGCG
GCAGTAGAGAACTTCTGGCTGCACATCGGCCCGTGTTTCGCGCTGGTTGCGCCTGACCGGGCGCACCTTCAGCGTTCGAT
GGAAGCACACAACCGTATCGTCGAGGCTTTGGCGGCGCGTGATGGGGCGGCGGCTCGTGCAGCTGTCACCGACGACATCA
TGCAGGCTGCCGACTCTCTGACGCGCCTGATCGCAAAGAGCGACCGCTCCAGGTCGCGTGTCTCGGGAGTGAAAAAAGCA
TGA

Upstream 100 bases:

>100_bases
GCTCCCACTGAGCACCCACGGTCTCCAAAAAAACAATTTGACATATGTTATTTGTGATCACATATTCGCTTCATAAGAAA
GCCAATATGAGTCACTCCCC

Downstream 100 bases:

>100_bases
GTGTTCTCAATCGGCTGGTTCCCTATGCAGGGCTGCGCGTCCTGATTTCCGGCGGTGCAGCCGGGATTGGTGAGGTGTTG
GCGGCGGCTTACCTGGAAGC

Product: transcriptional regulator GntR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MTDRPILLPPMRQVSRDTLQDQVYRQIREALMSGRFQPGQKLTIRGLAEALGSSPMPVREALNRLSAENAFEVTETARLR
VPMITPERLREIRDARVALEGLLAEKAVVLINDADLEDISQLCGQMQKAADSVDVALYLWTNFAFHRRIYAVAKAELIVA
AVENFWLHIGPCFALVAPDRAHLQRSMEAHNRIVEALAARDGAAARAAVTDDIMQAADSLTRLIAKSDRSRSRVSGVKKA

Sequences:

>Translated_240_residues
MTDRPILLPPMRQVSRDTLQDQVYRQIREALMSGRFQPGQKLTIRGLAEALGSSPMPVREALNRLSAENAFEVTETARLR
VPMITPERLREIRDARVALEGLLAEKAVVLINDADLEDISQLCGQMQKAADSVDVALYLWTNFAFHRRIYAVAKAELIVA
AVENFWLHIGPCFALVAPDRAHLQRSMEAHNRIVEALAARDGAAARAAVTDDIMQAADSLTRLIAKSDRSRSRVSGVKKA
>Mature_239_residues
TDRPILLPPMRQVSRDTLQDQVYRQIREALMSGRFQPGQKLTIRGLAEALGSSPMPVREALNRLSAENAFEVTETARLRV
PMITPERLREIRDARVALEGLLAEKAVVLINDADLEDISQLCGQMQKAADSVDVALYLWTNFAFHRRIYAVAKAELIVAA
VENFWLHIGPCFALVAPDRAHLQRSMEAHNRIVEALAARDGAAARAAVTDDIMQAADSLTRLIAKSDRSRSRVSGVKKA

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=210, Percent_Identity=29.5238095238095, Blast_Score=88, Evalue=4e-19,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 26637; Mature: 26506

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRPILLPPMRQVSRDTLQDQVYRQIREALMSGRFQPGQKLTIRGLAEALGSSPMPVRE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHH
ALNRLSAENAFEVTETARLRVPMITPERLREIRDARVALEGLLAEKAVVLINDADLEDIS
HHHHHCCCCCHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEECCCHHHHH
QLCGQMQKAADSVDVALYLWTNFAFHRRIYAVAKAELIVAAVENFWLHIGPCFALVAPDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCH
AHLQRSMEAHNRIVEALAARDGAAARAAVTDDIMQAADSLTRLIAKSDRSRSRVSGVKKA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCC
>Mature Secondary Structure 
TDRPILLPPMRQVSRDTLQDQVYRQIREALMSGRFQPGQKLTIRGLAEALGSSPMPVRE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHH
ALNRLSAENAFEVTETARLRVPMITPERLREIRDARVALEGLLAEKAVVLINDADLEDIS
HHHHHCCCCCHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEECCCHHHHH
QLCGQMQKAADSVDVALYLWTNFAFHRRIYAVAKAELIVAAVENFWLHIGPCFALVAPDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCH
AHLQRSMEAHNRIVEALAARDGAAARAAVTDDIMQAADSLTRLIAKSDRSRSRVSGVKKA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]